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Twenty castrated male Iberian pigs were divided into two groups of 10 pigs each according to the experimental diet received daily during the fattening period in confinement (acorn fed ad libitum or 4 kg of a formulated diet rich in C18:1 n-9). Dietary effects on performance, carcass characteristics and backfat, intramuscular, and liver fatty acid composition were assessed. Pigs fed only with acorns ate less, leading to a lower growth performance and carcass quality than pigs fed the formulated diet. Pigs fed acorns had lower C16:0 proportions in outer, inner, and subinner subcutaneous backfat layers. The C18:1 n-9 proportions in the inner and subinner subcutaneous backfat layers were higher in pigs fed acorns than in those fed the formulated diet. Pigs fed acorns had higher C18:2 n-6 proportion than those fed the formulated diet in outer subcutaneous backfat layer. The diet did not affect outer, inner, and subinner subcutaneous backfat melting points. Except a higher C18:0 in intramuscular fat polar lipids and higher C16:1 n-9 in the liver polar lipid fraction in pigs fed acorns, the diet had no significant effect on intramuscular and liver fatty acid composition. [ABSTRACT FROM AUTHOR]