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Two buffer-soluble endo-1,3-beta-D-glucanases (EC 3.2.1.6) have been purified to within 1% of electrophoretic homogeneity from etiolated Pisum sativum stem tissues. Purified glucanase I and II differ in physical properties, such as electrophoretic mobility in sodium dodecyl sulfate polyacrylamide gels (Mr values were 22 000 and 37 000, respectively) and isoelectric focusing, (pI values were 5.4 and 6.8, respectively). Although the enzymes have similar pH optima (5.5--6.0), Km values for various substrates (0.6--7.4 mg/ml) and thermal inactivation profiles, they are localized in different tissues and they differ markedly in the rates with which they attack the internal linkages of long- vs. short-chain substrates. Glucanase I is concentrated in apical regions of the stem and is most effectively assayed reductometrically (as laminarinase), while glucanase II is localized in mature regions and is relatively more active in viscometric assays (as carboxymethyl-pachymanase).

Two purified pea 1,3-beta-D-glucanases (EC 3.2.1.6) hydrolyse laminarin (degree of polymerization 20), laminaridextrins (degree of polymerization 3--7), and their reduced 3H-derivatives, 1,3-beta-D-glucans which are partially substituted (carboxymethyl-pachyman) or crystalline (curdlan), and mixed-linkage beta-glucans. Enzyme kinetics and product-formation indicate endo-hydrolase activity with weak transglycosylase capacity. The enzymes do not hydrolyse beta-glucosides, the 1,3 linkage adjacent to the reducing end of chains, or cellulose and its derivatives. They degrade mixed-linkage beta-glucans, in a manner similar to Rhizopus arrhizus endo-1,3-beta-D-glucanase, to form the products expected from hydrolysis of linkages adjacent to 1,3-beta linkages. With respect to action patterns, glucanase I (from apical growing tissue) differs from glucanase II (from basal maturing tissue) in several respects: (a) on a molar basis, I generates reducing groups from all substances more rapidly than II; (b) lower laminaridextrins are hydrolysed by I at the non-reducing terminal linkage, while II preferentially hydrolyses internal linkages; (c) laminarin is hydrolysed to lower laminaridextrins by I more rapidly than II, but I takes longer than II to completely degrade laminarin chains; (d) the enzymes are differentially sensitive to different classes of non-competitive inhibitors. It is concluded that these beta-glucanases differ in such a way that I preferentially continues to degrade fragments produced by endo-hydrolytic attack on long chains ('multiple attack' action pattern), while II hydrolyses internal linkages of the longest chains available ('multi-chain attack').