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Your search keyword '"Nawaz, Imtiaz M."' showing total 2 results
Start Over Author "Nawaz, Imtiaz M." Topic eye diseases
2 results on '"Nawaz, Imtiaz M."'

1. Human vitreous in proliferative diabetic retinopathy: Characterization and translational implications.

Author

Nawaz, Imtiaz M., Rezzola, Sara, Cancarini, Anna, Russo, Andrea, Costagliola, Ciro, Semeraro, Francesco, and Presta, Marco

Subjects
*DIABETIC retinopathy, *VISION disorders, *EYE diseases, *DISEASE progression, *RETINA, *POPULATION aging
Abstract

Diabetic retinopathy (DR) is one of the leading causes of visual impairment in the working-age population. DR is a progressive eye disease caused by long-term accumulation of hyperglycaemia-mediated pathological alterations in the retina of diabetic patients. DR begins with asymptomatic retinal abnormalities and may progress to advanced-stage proliferative diabetic retinopathy (PDR), characterized by neovascularization or preretinal/vitreous haemorrhages. The vitreous, a transparent gel that fills the posterior cavity of the eye, plays a vital role in maintaining ocular function. Structural and molecular alterations of the vitreous, observed during DR progression, are consequences of metabolic and functional modifications of the retinal tissue. Thus, vitreal alterations reflect the pathological events occurring at the vitreoretinal interface. These events are caused by hypoxic, oxidative, inflammatory, neurodegenerative, and leukostatic conditions that occur during diabetes. Conversely, PDR vitreous can exert pathological effects on the diabetic retina, resulting in activation of a vicious cycle that contributes to disease progression. In this review, we recapitulate the major pathological features of DR/PDR, and focus on the structural and molecular changes that characterize the vitreal structure and composition during DR and progression to PDR. In PDR, vitreous represents a reservoir of pathological signalling molecules. Therefore, in this review we discuss how studying the biological activity of the vitreous in different in vitro, ex vivo, and in vivo experimental models can provide insights into the pathogenesis of PDR. In addition, the vitreous from PDR patients can represent a novel tool to obtain preclinical experimental evidences for the development and characterization of new therapeutic drug candidates for PDR therapy. [ABSTRACT FROM AUTHOR]

Published
2019
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2. Inflammation and N-formyl peptide receptors mediate the angiogenic activity of human vitreous humour in proliferative diabetic retinopathy

Author

Paola Chiodelli, Stefania Mitola, Daniela Coltrini, Sara Rezzola, Mirella Belleri, Mario De Rosa, Francesco Semeraro, Vincenzo Pavone, Anna Cancarini, Michela Corsini, Roberto Ronca, Marco Presta, Dario Rusciano, Imtiaz M. Nawaz, Luca Lista, Rezzola, Sara, Corsini, Michela, Chiodelli, Paola, Cancarini, Anna, Nawaz, Imtiaz M., Coltrini, Daniela, Mitola, Stefania, Ronca, Roberto, Belleri, Mirella, Lista, Liliana, Rusciano, Dario, de Rosa, Mario, Pavone, Vincenzo, Semeraro, Francesco, and Presta, Marco

Subjects
0301 basic medicine, Male, endocrine system diseases, Angiogenesis, Endocrinology, Diabetes and Metabolism, Endothelial cells, Pharmacology, Vitreous, Pathogenesis, Mice, Endocrinology, Endothelial cell, Diabetic retinopathy, Receptor, Aged, 80 and over, Neovascularization, Pathologic, Middle Aged, Diabetes and Metabolism, Angiogenesi, medicine.anatomical_structure, Inflammation, N-formyl peptide receptor, Internal Medicine, Female, medicine.symptom, Endothelium, Proinflammatory cytokine, Cell Line, 03 medical and health sciences, medicine, Human Umbilical Vein Endothelial Cells, Animals, Humans, cardiovascular diseases, Aged, Vitreou, Vitreous humour, business.industry, medicine.disease, Receptors, Formyl Peptide, eye diseases, Vitreous Body, 030104 developmental biology, Immunology, sense organs, business
Abstract

AIMS/HYPOTHESIS: Angiogenesis and inflammation characterise proliferative diabetic retinopathy (PDR), a major complication of diabetes mellitus. However, the impact of inflammation on the pathogenesis of PDR neovascularisation has not been elucidated. Here, we assessed the capacity of PDR vitreous fluid to induce pro-angiogenic/proinflammatory responses in endothelium and the contribution of the inflammation-related pattern recognition N-formyl peptide receptors (FPRs) in mediating these responses. METHODS: Pooled and individual pars plana vitrectomy-derived PDR vitreous fluid ('PDR vitreous') samples were assessed in endothelial cell proliferation, motility, sprouting and morphogenesis assays, and for the capacity to induce proinflammatory transcription factor activation, reactive oxygen species production, intercellular junction disruption and leucocyte-adhesion molecule upregulation in these cells. In vivo, the pro-angiogenic/proinflammatory activity of PDR vitreous was tested in murine Matrigel plug and chick embryo chorioallantoic membrane (CAM) assays. Finally, the FPR inhibitors Boc-Phe-Leu-Phe-Leu-Phe (Boc-FLFLF) and Ac-L-Arg-Aib-L-Arg-L-Cα(Me)Phe-NH2 tetrapeptide (UPARANT) were evaluated for their capacity to affect the biological responses elicited by PDR vitreous. RESULTS: PDR vitreous activates a pro-angiogenic/proinflammatory phenotype in endothelial cells. Accordingly, PDR vitreous triggers a potent angiogenic/inflammatory response in vivo. Notably, the different capacity of individual PDR vitreous samples to induce neovessel formation in the CAM correlates with their ability to recruit infiltrating CD45+ cells. Finally, the FPR inhibitor Boc-FLFLF and the novel FPR antagonist UPARANT inhibit neovessel formation and inflammatory responses triggered by PDR vitreous in the CAM assay. CONCLUSIONS/INTERPRETATION: This study provides evidence that inflammation mediates the angiogenic activity of PDR vitreous and paves the way for the development of FPR-targeting anti-inflammatory/anti-angiogenic approaches for PDR therapy.

Published
2017

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