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In this study, a smart and green analytical method based on the second-order calibration algorithm coupled with excitation-emission matrix (EEM) fluorescence was developed for the determination of rhodamine dyes illegally added into chilli samples. The proposed method not only has the advantage of high sensitivity over the traditional fluorescence method but also fully displays the “second-order advantage”. Pure signals of analytes were successfully extracted from severely interferential EEMs profiles via using alternating trilinear decomposition (ATLD) algorithm even in the presence of common fluorescence problems such as scattering, peak overlaps and unknown interferences. It is worth noting that the unknown interferents can denote different kinds of backgrounds, not only refer to a constant background. In addition, the method using interpolation method could avoid the information loss of analytes of interest. The use of “mathematical separation” instead of complicated “chemical or physical separation” strategy can be more effective and environmentally friendly. A series of statistical parameters including figures of merit and RSDs of intra- (≤1.9%) and inter-day (≤6.6%) were calculated to validate the accuracy of the proposed method. Furthermore, the authoritative method of HPLC-FLD was adopted to verify the qualitative and quantitative results of the proposed method. Compared with the two methods, it also showed that the ATLD-EEMs method has the advantages of accuracy, rapidness, simplicity and green, which is expected to be developed as an attractive alternative method for simultaneous and interference-free determination of rhodamine dyes illegally added into complex matrices. [ABSTRACT FROM AUTHOR]

Abstract A new strategy for the determination of bisphenol A (BPA) and diphenyl carbonate (DPC) in polycarbonate plastics has been proposed, combing excitation-emission matrix fluorescence and second-order chemometrics methods. The studied compounds are two of the most frequently used chemical raw materials and intermediates, which is mainly used for bio-chemical products. The method is fast and sensitive as it can avoid tedious pretreatment steps and large amounts of organic solvents consumption. Chemometrics allowed accurate and precise quantification of two target analytes even in the presence of severe spectral overlap, unknown and background interferences, which benefit from "second-order advantages" provided by chemometrics multivariate calibration. Reasonable quantification results were obtained in real plastics samples, with limits of detection for two analytes were 0.04 and 1.18 × 103 ng mL−1 and limits of quantitation were 0.11 and 3.58 × 103 ng mL−1. In addition, the average recoveries for BPA and DPC were 99.35% and 83.50% with relative standard deviation <2.30%. It was proved that the proposed method can be a useful and sensitive tool to the determination of BPA and DPC in different polycarbonate plastics with a simple sample pretreatment at low cost. Graphical abstract Unlabelled Image Highlights • A fast chemometrics-assisted EEM fluorescence method was used to the determination of BPA and DPC in PC plastics. • Satisfactory results were confirmed that the method is an smart, fast, accurate, green strategy. • The method can be used to the determination of BPA and DPC in different PC plastics at low cost. [ABSTRACT FROM AUTHOR]

The matter of fluoroquinolone residues in various foods still arouses wide public concern nowadays. In the present work, the strategy of excitation-emission matrix (EEM) fluorescence data coupled with second-order calibration method based on alternating normalization-weighted error (ANWE) algorithm was used to determine ofloxacin, lomefloxacin and ciprofloxacin in milk powder, milk and beef. Owning the unique "second-order advantage", the ANWE-assisted analytical method was proved to successfully and eco-friendly resolve the overlapped fluorescence spectra of multi-component in complex food matrixes without tedious pretreatment steps and sophisticated high-cost instrumentations. The feasibility of the proposed method was validated by experiments. The average spiked recoveries of three fluoroquinolones range from 82.6% to 110.5% with relative standard deviations lower than 7.4%, and the limits of detection range from 0.18 and 2.41 ng mL−1. For further evaluation, analytical figures of merit such as sensitivity and selectivity, as well as the RSDs of intra-day (≦10.6%) and inter-day (≦9.4%) were calculated. The satisfactory analytical results demonstrated that the proposed strategy could be a competitive alternative for simple, rapid and simultaneous determination of multiple fluoroquinolones in animal-derived food samples. Unlabelled Image • An ANWE-assisted EEM fluorescence method was developed to rapidly quantify three fluoroquinolones in foods. • Highly overlapped fluorescence spectra were well-resolved via "mathematical separation". • Satisfactory results indicated that the proposed method was fast, accurate and eco-friendly. [ABSTRACT FROM AUTHOR]