1. The toxicity mechanism of toxic compounds from Euphorbiae pekinensis Radix on zebrafish embryos.
- Author
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Qin WN, Zhang KC, Geng T, Cheng FF, Chen PD, Yao WF, Zhang L, and Cao YD
- Subjects
- Animals, Apoptosis drug effects, Apoptosis Regulatory Proteins genetics, Apoptosis Regulatory Proteins metabolism, Cardiotoxicity, Embryo, Nonmammalian drug effects, Embryo, Nonmammalian metabolism, Embryo, Nonmammalian pathology, Euphorbia chemistry, Gastrointestinal Tract embryology, Gastrointestinal Tract metabolism, Heart embryology, Liver embryology, Liver metabolism, Oxidative Stress drug effects, Phytochemicals isolation & purification, Plant Extracts isolation & purification, Zebrafish Proteins genetics, Zebrafish Proteins metabolism, Euphorbia toxicity, Gastrointestinal Tract drug effects, Heart drug effects, Liver drug effects, Phytochemicals toxicity, Plant Extracts toxicity, Zebrafish embryology
- Abstract
Euphorbiae pekinensis Radix (EP) is effective in treating various diseases, but it's toxicity is a major obstacle in use in clinical. Although EP was processed with vinegar to reduce it's toxicity, the detailed mechanism of toxicity in EP have not been clearly delineated. This study investigate the toxicity attenuation-mechanism of Euphorbiae pekinensis after being processed with vinegar (VEP) and the toxic mechanism of four compounds from EP on zebrafish embryos. The contents of four compounds decreased obviously in VEP. Correspondingly, slower development on embryos can be seen as some symptoms like reduction of heart rate, liver area and gastrointestinal peristalsis after exposed to the compounds. Some obvious pathological signals such as pericardial edema and yolk sac edema were observed. Furthermore, the compounds could increase the contents of MDA and GSH-PX and induce oxidative damage by inhibiting the activity of SOD. Also, four compounds could provoke apoptosis by up-regulating the expression level of p53, MDM2, Bax, Bcl-2 and activating the activity of caspase-3, caspase-9. In conclusion, the four compounds play an important role in the toxicity attenuation effects of VEP, which may be related to the apoptosis induction and oxidative damage. This would contribute to the clinical application and further toxicity-reduction mechanism research., (Copyright © 2021 The Authors. Published by Elsevier Masson SAS.. All rights reserved.)
- Published
- 2021
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