1. Direct Nuclear Delivery of DNA by Photothermal Nanoblade.
- Author
-
Wu TH, Wu YC, Sagullo E, Teitell MA, and Chiou PY
- Subjects
- Animals, Cell Survival, Gene Expression, Humans, Lasers, Mammals, Microbubbles, Nanotechnology methods, Cell Nucleus metabolism, DNA genetics, DNA metabolism, Eukaryotic Cells, Microinjections methods, Transfection methods, Transformation, Genetic
- Abstract
We demonstrate direct nuclear delivery of DNA into live mammalian cells using the photothermal nanoblade. Pulsed laser-triggered cavitation bubbles on a titanium-coated micropipette tip punctured both cellular plasma and nuclear membranes, which was followed by pressure-controlled delivery of DNA into the nucleus. High-level and efficient plasmid expression in different cell types with maintained cell viability was achieved., (© 2015 Society for Laboratory Automation and Screening.)
- Published
- 2015
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