Search

Background: We aimed to determine the household distribution and viability of Chlamydia trachomatis (Ct) from the eyes, face, and hands during the initial two visits of a year-long fortnightly cohort study in geographically defined adjacent households. Methods/Findings: We enrolled 298 individuals from 68 neighbouring households in Shashemene Woreda, Oromia, Ethiopia. All individuals above 2 years of age residing in these households were examined for signs of trachoma. Swab samples were taken from the conjunctiva, faces, and hands and analysed for the presence and viability of Ct. Ct viability was determined using reverse transcription (RT) PCR. At the initial visit, out of 298 individuals, 133 (44.5%) were children aged 2–9 years. Among these children, 27/133 (20.3%) had trachomatous inflammation—follicular (TF), while 8/133 (6.0%) had trachomatous inflammation—intense (TI). Ct (omcB or pORF2) was detected in 16/133 (12.0%) eye swabs, 14/105 (13.5%) face swabs, and 11/105 (10.5%) hand swabs from children aged 2–9 years. Among these children at visit one, 12/14 (85.7%) with Ct on faces and 9/11 (81.8%) with Ct on hands also had detectable ocular Ct. The severity of the disease worsened from the first visit to the second, and no participants showed clearance of the disease within the two-week period. Ct infection was associated with TF (P = 0.002) and TI (P = 0.060). At visit one, among children aged 2–9 years, viable Ct was detected in 12/16 (75.0%) ocular, 6/14 (42.9%) face, and 4/11 (36.4%) hand swab samples. All viable Ct detected on the faces and hands were identified from individuals with viable ocular infections. Among caregivers whose child tested positive for Ct on their hands, 3 caregivers also had Ct on their hands, accounting for 20% (3 out of 15). Additionally, among caregivers whose child tested positive for Ct on their faces, 2 caregivers had Ct on their faces, which accounts for 14.3% (2 out of 14). In two participants, we detected Ct on the hands of ocular-negative children at the initial visit and later detected ocular Ct at the second visit. Conclusion/Significance: Using RT-qPCR assay to detect Ct omp2 mRNA to define viability offers a new, informative perspective of trachoma transmission in this community in Ethiopia. The presence of viable Ct on the faces and hands of individuals living in households with people with current ocular Ct infection supports the hypothesis that hands and faces are important routes for transmission of trachoma. This highlights the importance of targeted interventions to address these sites of Ct carriage to help interrupt transmission. Author summary: Trachoma, caused by Chlamydia trachomatis (Ct), remains a leading cause of infectious blindness globally. Understanding how Ct is transmitted within households is crucial for designing effective interventions. In this study, we investigated the presence and viability of Ct on the eyes, faces, and hands of individuals in geographically adjacent households in Shashemene Woreda, Oromia, Ethiopia, over two visits during a year-long cohort study. We enrolled 298 participants from 68 neighbouring households, including 133 children aged 2–9 years. Trachomatous inflammation—follicular (TF) was observed in 20.3% of these children, and trachomatous inflammation—intense (TI) was found in 6.0%. Ct was detected in ocular, facial, and hand swabs, with a strong association between ocular Ct infection and the presence of viable Ct on the face and hands. Notably, 75.0% of ocular Ct infections in children were viable, as were 42.9% and 36.4% of infections on the face and hands, respectively. Viable Ct on the face and hands was predominantly identified in individuals who also had viable ocular infections. Caregivers of children with Ct on their faces or hands occasionally carried Ct themselves, underscoring potential intra-household transmission. We also observed cases where Ct on the hands preceded ocular infection, supporting the hypothesis that hands and faces play a critical role in disease spread. By utilizing reverse transcription PCR to detect viable Ct, this study provides an insights into transmission dynamics in this Ethiopian community. The findings emphasize the need for targeted interventions focusing on facial and hand hygiene to disrupt Ct transmission and support ongoing trachoma elimination efforts. [ABSTRACT FROM AUTHOR]