1. Reversal of ethanol-induced hepatotoxicity by cinnamic and syringic acids in mice.
- Author
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Yan SL, Wang ZH, Yen HF, Lee YJ, and Yin MC
- Subjects
- Alanine Transaminase metabolism, Animals, Anti-Infective Agents, Local toxicity, Antioxidants metabolism, Blotting, Western, Cyclooxygenase 2 metabolism, Cytochrome P-450 CYP2E1 metabolism, Cytokines metabolism, Gallic Acid pharmacology, Hepatitis, Alcoholic etiology, Hepatitis, Alcoholic metabolism, Immunoenzyme Techniques, Male, Mice, Mice, Inbred BALB C, NF-E2-Related Factor 2 metabolism, Reactive Oxygen Species metabolism, Tumor Necrosis Factor-alpha metabolism, Cinnamates pharmacology, Ethanol toxicity, Gallic Acid analogs & derivatives, Hepatitis, Alcoholic prevention & control, Oxidative Stress drug effects
- Abstract
Ethanol was used to induce acute hepatotoxicity in mice. Effects of cinnamic acid (CA) and syringic acid (SA) post-intake for hepatic recovery from alcoholic injury was investigated. Ethanol treated mice were supplied by CA or SA at 40 or 80 mg/kg BW/day for 5 days. Results showed that ethanol stimulated protein expression of CYP2E1, p47
phox , gp91phox , cyclooxygenase-2 and nuclear factor kappa B in liver. CA or SA post-intake restricted hepatic expression of these molecules. Ethanol suppressed nuclear factor erythroid 2-related factor (Nrf2) expression, and CA or SA enhanced Nrf2 expression in cytosolic and nuclear fractions. Ethanol increased the release of reactive oxygen species, oxidized glutathione, interleukin-6, tumor necrosis factor-alpha, nitric acid and prostaglandin E2 . CA or SA lowered hepatic production of these oxidative and inflammatory factors. Histological data revealed that ethanol administration caused obvious foci of inflammatory cell infiltration, and CA or SA post-intake improved hepatic inflammatory infiltration. These findings support that cinnamic acid and syringic acid are potent nutraceutical agents for acute alcoholic liver disease therapy. However, potential additive or synergistic benefits of cinnamic and syringic acids against ethanol-induced hepatotoxicity need to be investigated., (Copyright © 2016 Elsevier Ltd. All rights reserved.)- Published
- 2016
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