13 results on '"De Martino, Laura"'
Search Results
2. Origanum heracleoticum Essential Oils: Chemical Composition, Phytotoxic and Alpha-Amylase Inhibitory Activities.
- Author
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Amato, Giuseppe, Caputo, Lucia, Francolino, Rosaria, Martino, Mara, De Feo, Vincenzo, and De Martino, Laura
- Subjects
ESSENTIAL oils ,ALPHA-amylase ,ORIGANUM ,ITALIAN ryegrass ,PHYTOTOXICITY ,GERMINATION - Abstract
Many studies have demonstrated the herbicidal effects of several essential oils and their possible use as substitutes for chemical herbicides. Several enzymes play a very significant role in seed germination: among these, α-amylase could be involved in essential oil phytotoxic processes. The aims of this study were to compare the chemical composition of the essential oils of two ecotypes of O. heracleoticum growing in Cilento (Southern Italy) and to study their possible use as natural herbicide using Raphanus sativus, Sinapis arvensis and Lolium multiflorum seeds. Moreover, a possible inhibitory activity on the α-amylase enzyme extracted from germinating seeds was evaluated as a possible mechanism of action. Both oils, characterized by GC-MS, belonged to a carvacrol chemotype. The alpha-amylase activity was determined using DNSA (dinitrosalicylic acid) assay quantifying the reducing sugar produced. Furthermore, the essential oils demonstrated phytotoxicity at the highest dose tested, and an inhibitory effect on α-amylase, probably correlated with the phytotoxic effects, was registered. The oils showed interesting phytotoxic and alpha-amylase inhibitory activities, which deserve to be further investigated. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
- View/download PDF
3. Postharvest Microwave Drying of Basil (Ocimum basilicum L.): The Influence of Treatments on the Quality of Dried Products.
- Author
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De Martino, Laura, Caputo, Lucia, Amato, Giuseppe, Iannone, Marco, Barba, Anna Angela, and De Feo, Vincenzo
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BASIL ,MICROWAVE drying ,MICROWAVE heating ,ESSENTIAL oils ,PRODUCT quality ,ELECTROMAGNETIC fields - Abstract
Edible herbs are widely used in the human diet due to their pleasant flavors and countless health benefits associated with their components having, mainly, antioxidant and anti-inflammatory therapeutic functions. Since herbs are highly perishable materials because of their high water content, to guarantee products are safe and stable over time, it is necessary that they undergo stabilization operations. The application of microwave-assisted drying, a promising technique in terms of process sustainability, for the stabilization of the aromatic herb, Ocimum basilicum L., was investigated. The activities were carried out by applying different operating conditions in order to evaluate the impact of the time/temperature combination on the final quality of dried basil. The latter was investigated via the chemical characterization of extracted essential oils and tissue damages. Conventional convective processes were also applied to perform comparisons between dried basil products both under production and the quality preservation points of view. Results showed that microwave heating is suitable as a drying method, as expected, due to the well-known interaction between vegetable tissue (rich in water) and the electromagnetic field; and that drying methods have a different influence on the chemical composition of the essential oils extracted from dried products, in terms of the number (ranging from 41 to 18 components in different dried samples) and percentage (until 67% in linalool and 21% in α-trans-bergamotene in different dried samples) of its' constituents. [ABSTRACT FROM AUTHOR]
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- 2022
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4. Laurus nobilis: Composition of Essential Oil and Its Biological Activities.
- Author
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Caputo, Lucia, Nazzaro, Filomena, Souza, Lucéia Fatima, Aliberti, Luigi, De Martino, Laura, Fratianni, Florinda, Coppola, Raffaele, and De Feo, Vincenzo
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LAURUS nobilis ,ESSENTIAL oils ,MEDICINAL plants ,ORNAMENTAL plants ,ANTI-infective agents ,IN vitro studies ,CELL-mediated cytotoxicity - Abstract
Laurus nobilis is native to the southern Mediterranean region and cultivated mainly in Europe and the USA as an ornamental and medicinal plant. The chemical composition of the essential oil (EO) from leaves of L. nobilis, collected in Southern Italy, was studied by GC and GC-MS. In all, 55 compounds were identified, accounting for 91.6% of the total essential oil. 1,8-Cineole (31.9%), sabinene (12.2%), and linalool (10.2%) were the main components. Antimicrobial and antifungal activities of EO and 1,8-cineole were determined in vitro. The cytotoxicity of the EO was evaluated against SH-SY5Y cell line, as well as the influence of the EO on the expression of adenylate cyclase 1 (ADCY1), suggesting possible essential oil effects on the Central Nervous System. [ABSTRACT FROM AUTHOR]
- Published
- 2017
- Full Text
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5. Chemical Composition and in Vitro Antimicrobial, Cytotoxic, and Central Nervous System Activities of the Essential Oils of Citrus medica L. cv. 'Liscia' and C. medica cv. 'Rugosa' Cultivated in Southern Italy.
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Aliberti, Luigi, Caputo, Lucia, De Feo, Vincenzo, De Martino, Laura, Nazzaro, Filomena, and Souza, Lucéia Fátima
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CITRON ,THERAPEUTIC use of essential oils ,ANTI-infective agents ,ADENYLATE cyclase ,LIMONENE ,BACILLUS cereus ,STAPHYLOCOCCUS aureus ,PSEUDOMONAS aeruginosa - Abstract
Citrus medica cv. 'liscia' and C. medica cv. 'rugosa' are two taxa of citron, belonging to the biodiversity of South Italy, in particular of Amalfi Coast, in the Campania region. The chemical composition of the essential oils (EOs) from fruit peels of both C. medica cultivars was studied by gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS) analyses. In all, 100 compounds were identified, 82 for C. medica cv. 'liscia', accounting for 91.4% of the total oil, and 88 for C. medica cv. 'rugosa', accounting for 92.0% of the total oil. Monoterpene hydrocarbons are the main constituents in both oils of C. medica cv. 'liscia' (79.1%) and C. medica cv. 'rugosa' (80.2%). In both oils, limonene (67.2%-62.8%) and camphene (8.5%-10.9%) are the main constituents. The antimicrobial activity of the EOs was assayed against some bacterial strains: Bacillus cereus (DSM 4313), Bacillus cereus (DSM 4384), Staphylococcus aureus (DSM 25693), Pseudomonas aeruginosa (ATCC 50071), and Escherichia coli (DSM 8579). Low concentrations of C. medica cv. 'rugosa' EO showed an inhibitory effect on P. aeruginosa and higher concentrations inhibited more B. cereus (4384) and E. coli than S. aureus. The cytotoxicity of the EO was evaluated against SH-SY5Y cell line. The influence of the EO on the expression of adenylate cyclase 1 (ADCY1) was also studied. The antimicrobial activity registered confirm their traditional uses as food preserving agents and led us to hypothesize the possible use of these oils as antimicrobials. The alterations in ADCY1 expression suggested a role for limonene in effects on the central nervous system. [ABSTRACT FROM AUTHOR]
- Published
- 2016
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6. Studies on Chemical Composition, Antimicrobial and Antioxidant Activities of Five Thymus vulgaris L. Essential Oils.
- Author
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Mancini, Emilia, Senatore, Federica, Del Monte, Donato, De Martino, Laura, Grulova, Daniela, Scognamiglio, Mariarosa, Snoussi, Mejdi, and De Feo, Vincenzo
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ANTIOXIDANT analysis ,ESSENTIAL oils ,FUNCTIONAL foods ,FOOD preservatives ,GALLIC acid - Abstract
This study is aimed at assessing the essential oil composition, total phenolic content, antimicrobial and antioxidant activities of Thymus vulgaris collected in five different area of the Campania Region, Southern Italy. The chemical composition of the essential oils was studied by GC-flame ionization detector (FID) and GC/MS; the biological activities were evaluated through determination of MIC and minimum bactericidal concentration (MBC) and evaluation of antioxidant activity. In total, 134 compounds were identified. The oils were mainly composed of phenolic compounds, and all oils belonged to the chemotype thymol. The antimicrobial activity of the five oils was assayed against ten bacterial strains. The oils showed different inhibitory activity against some Gram-positive pathogens. The total phenol content in the essential oils ranged from 77.6-165.1 mg gallic acid equivalents (GAE)/g. The results reported here may help to shed light on the complex chemotaxonomy of the genus Thymus. These oils could be used in many fields as natural preservatives of food and as nutraceuticals. [ABSTRACT FROM AUTHOR]
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- 2015
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7. Chemical Composition and Biological Activity of the Essential Oil from Leaves of Moringa oleifera Lam. Cultivated in Mozambique.
- Author
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Marrufo, Tatiana, Nazzaro, Filomena, Mancini, Emilia, Fratianni, Florinda, Coppola, Raffaele, De Martino, Laura, Agostinho, Adelaide Bela, and De Feo, Vincenzo
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MORINGA oleifera ,ESSENTIAL oils ,CHEMICAL composition of plants ,ANTIOXIDANTS ,CHROMATOGRAPHIC analysis - Abstract
The antioxidant capacity and antimicrobial activity of the essential oil of Moringa oleifera (Moringaceae) grown in Mozambique was investigated. The chemical composition was studied by means of GC and GC-MS analysis. Hexacosane (13.9%), pentacosane (13.3%) and heptacosane (11.4%) were the main components. Ultra High Performance Chromatography-DAD analysis detected the flavonoids quercetin (126 μg/g) and luteolin (6.2 μg/g). The essential oil exhibited a relatively low free radical scavenging capacity. The antimicrobial activity of the essential oil was assayed against two Gram-positive strains (Bacillus cereus, Staphylococcus aureus), two Gram-negative strains (Escherichia coli, Pseudomonas aeruginosa), and five fungal strains of agro-food interest (Penicillium aurantiogriseum, Penicillium expansum, Penicillium citrinum, Penicillium digitatum, and Aspergillus niger spp.). B. cereus and P. aeruginosa, as well as the fungal strains were sensitive to the essential oil. [ABSTRACT FROM AUTHOR]
- Published
- 2013
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8. Chemical Composition and Possible in Vitro Phytotoxic Activity of Helichrsyum italicum (Roth) Don ssp. italicum.
- Author
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Mancini, Emilia, De Martino, Laura, Marandino, Aurelio, Scognamiglio, Maria Rosa, and De Feo, Vincenzo
- Subjects
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HELICHRYSUM , *GAS chromatography/Mass spectrometry (GC-MS) , *SPECTRUM analysis , *PHYTOTOXICITY , *GERMINATION ,SPECTRA of essential oils - Abstract
The chemical composition of the essential oil of Helichrysum italicum (Roth) Don ssp. italicum, collected in the National Park of Cilento and Diano Valley, Southern Italy, was studied by means of GC and GC/MS. Forty four compounds of 45 constituents were identified in the oil, mainly oxygenated sesquiterpenes. The essential oil was evaluated for its potential in vitro phytotoxic activity against germination and early radicle elongation of radish and garden cress. The radicle elongation of radish was significantly inhibited at the highest doses tested, while germination of both seeds was not affected. [ABSTRACT FROM AUTHOR]
- Published
- 2011
- Full Text
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9. Chemical Composition and Antigerminative Activity of the Essential Oils from Five Salvia Species.
- Author
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De Martino, Laura, Roscigno, Graziana, Mancini, Emilia, De Falco, Enrica, and De Feo, Vincenzo
- Subjects
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SALVIA , *ESSENTIAL oils , *LEPIDIUM , *MONOTERPENES , *SESQUITERPENES - Abstract
The chemical composition of the essential oils of Salvia africana L., Salvia elegans Vahl, Salvia greggii A. Gray, Salvia mellifera Green and Salvia munzii Epling, cultivated in Eboli (Salerno, Southern Italy), was studied by means of GC and GC-MS analyses. In all, 88 compounds were identified, 54 for S. africana, accounting for 95.4% of the total oil, 55 for S. elegans (92.9%), 50 for S. greggii (96.9%), 54 for S. mellifera (90.4%) and 47 for S. munzii (97.5%), respectively. In S. africana, the amount of monoterpenoids and sesquiterpenoids is very similar. For other species, the monoterpenoid percentage is greater than the amount of sesquiterpenoids. The oils of S. elegans, S. greggii and S. munzii were active inhibitors of germination and radical elongation of Raphanus sativus L. and Lepidium sativum L. [ABSTRACT FROM AUTHOR]
- Published
- 2010
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10. Chemical Composition and Phytotoxic Effects of Essential Oils of Salvia hierosolymitana Boiss. and Salvia multicaulis Vahl. var. simplicifolia Boiss. Growing Wild in Lebanon.
- Author
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Mancini, Emilia, Arnold, Nelly Apostolides, De Martino, Laura, De Feo, Vincenzo, Formisano, Carmen, Rigano, Daniela, and Senatore, Felice
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BRASSICACEAE ,MONOTERPENES ,SESQUITERPENES ,GERMINATION ,PHYTOTOXINS ,PLANT growth ,ALLELOCHEMICALS ,AROMATIC plants ,ALLELOPATHY - Abstract
The chemical composition of the essential oils of S. hierosolymitana Boiss. and S. multicaulis Vahl. var. simplicifolia Boiss. collected in Lebanon was studied by means of GC and GC-MS analysis. In all 115 compounds were identified: 82 for S hierosolymitana and 72 for S. multicaulis var. simplicifolia. The presence of carbonylic compounds (17%) characterizes the oil from S. hierosolymitana, while S. multicaulis var. simplicifolia oil is rich of monoterpenes (34.5%) and sesquiterpenes (46.9%). The effects of the essential oils on germination and initial radical elongation of Raphanus sativus L. (radish) and Lepidium sativum L. (garden cress) were studied, indicating in a different activity against radical elongation of the species tested. [ABSTRACT FROM AUTHOR]
- Published
- 2009
- Full Text
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11. Valorization of Sweet Lime Peel for the Extraction of Essential Oil by Solvent Free Microwave Extraction Enhanced with Ultrasound Pretreatment.
- Author
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Arafat, Yasir, Altemimi, Ammar, Ibrahim, Salam Adnan, Badwaik, Laxmikant Shivnath, and De Martino, Laura
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ESSENTIAL oils ,MICROWAVES ,MICROWAVE spectroscopy ,VACUUM - Abstract
Essential oils of sweet lime peel, a waste by-product in the juice industry, were extracted using the vacuum assisted solvent free microwave extraction (VASFME) method. The effects of microwave output power (500–1000 W) and extraction time (20–30 min) on the essential oils yield and antimicrobial property were investigated. Optimal conditions were observed at 797.844 W microwave output power and 30 min extraction time. The essential oils yield and antimicrobial property under these conditions were 0.792 ± 0.03% and 18.25 ± 1.45 mm, respectively, which agrees with the predicted values of 0.757% and 16.50 mm. The essential oils were extracted at optimized conditions and analyzed through GCMS for compound identification. A total of 49 compounds were identified, with limonene content (43.47%) being the highest among all sweet lime peel oil compounds. Moreover, the sweet lime peels were subjected to ultrasound pre-treatment before microwave extraction. The ultrasound pre-treatment helped to increase the essential oils yield from 0.84 to 1.06% as the treatment time increased from 30 to 90 min. The increase in yield was 37.66% more compared to VASFME at 90 min treatment time. [ABSTRACT FROM AUTHOR]
- Published
- 2020
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12. The Essential Oil Compositions of Three Teucrium Taxa Growing Wild in Sicily: HCA and PCA Analyses
- Author
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Laura De Martino, Vincenzo Ilardi, Natale Badalamenti, Maurizio Bruno, Giorgia Catinella, Sergio Rosselli, and Catinella Giorgia, Badalamenti Natale, Ilardi Vincenzo, Rosselli Sergio, De Martino Laura, Bruno, Maurizio
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Monoterpene ,Teucrium flavum L ,Pharmaceutical Science ,Biology ,Sesquiterpene ,01 natural sciences ,essential oil ,Article ,Gas Chromatography-Mass Spectrometry ,Analytical Chemistry ,law.invention ,Teucrium ,lcsh:QD241-441 ,chemistry.chemical_compound ,lcsh:Organic chemistry ,Species Specificity ,Genus ,law ,Drug Discovery ,Botany ,Oils, Volatile ,Cluster Analysis ,Plant Oils ,Physical and Theoretical Chemistry ,Teucrium montanum L ,Chemical composition ,essential oils ,Sicily ,Essential oil ,PCA ,Principal Component Analysis ,010405 organic chemistry ,Organic Chemistry ,Plant Components, Aerial ,biology.organism_classification ,Teucrium capitatum L ,0104 chemical sciences ,010404 medicinal & biomolecular chemistry ,chemotaxonomy ,chemistry ,Chemistry (miscellaneous) ,Chemotaxonomy ,Molecular Medicine ,Composition (visual arts) ,GC-MS - Abstract
The chemical composition and the qualitative and quantitative variability of the essential oils of three taxa belonging to the Teucrium genus were studied. The investigated taxa, that grow wild in Sicily, were Teucrium flavum L. (section Chamaedrys (Mill.) Scheb.), Teucrium montanum and Teucrium capitatum L. of section Polium (Mill.) Scheb. Essential oils were extracted by hydrodistillation and analyzed by GC-MS. In total, 74 compounds were identified. Sesquiterpene hydrocarbons were found to be the main group for T. flavum (48.3%). T. capitatum consisted essentially of monoterpene hydrocarbons (72.7%), with &alpha, pinene (19.9%), &beta, pinene (27.6%) and sylvestrene (16.6%) as the most abundant compounds whereas ledene oxide (12.1%), epiglobulol (13.5%) and longifolenaldehyde (14.5%) were identified as the main constituents among the oxygenated sesquiterpenes (63.5%) of T. montanum. Furthermore, a complete literature review on the composition of the essential oils of all the other accessions of these Teucrium taxa, studied so far, was performed. Hierarchical Cluster Analysis (HCA) and Principal Component Analyses (PCA) were used in order to demonstrate geographical variations in the composition of the essential oils.
- Published
- 2021
13. Chemical Composition and in Vitro Antimicrobial, Cytotoxic, and Central Nervous System Activities of the Essential Oils of Citrus medica L. cv. ‘Liscia’ and C. medica cv. ‘Rugosa’ Cultivated in Southern Italy
- Author
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Laura De Martino, Lucéia Fátima Souza, Vincenzo De Feo, Luigi Aliberti, Lucia Caputo, and Filomena Nazzaro
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0301 basic medicine ,Citrus ,Monoterpene ,Bacillus cereus ,Volatile ,Pharmaceutical Science ,SH-SY5Y cells ,Analytical Chemistry ,law.invention ,chemistry.chemical_compound ,0302 clinical medicine ,Citrus medica ,adenylate cyclase ,antimicrobial activity ,essential oil ,Adenylyl Cyclases ,Bacteria ,Cell Line, Tumor ,Fruit ,Humans ,Italy ,Anti-Infective Agents ,Central Nervous System Agents ,Cytotoxins ,Oils, Volatile ,law ,Drug Discovery ,Tumor ,Traditional medicine ,biology ,Antimicrobial ,food safety ,Cereus ,Chemistry (miscellaneous) ,Molecular Medicine ,Camphene ,Article ,Cell Line ,lcsh:QD241-441 ,03 medical and health sciences ,food ,lcsh:Organic chemistry ,Botany ,Physical and Theoretical Chemistry ,essential oils ,Essential oil ,Limonene ,Organic Chemistry ,biology.organism_classification ,food.food ,030104 developmental biology ,chemistry ,antimicrobial ,Oils ,030217 neurology & neurosurgery - Abstract
Citrus medica cv. 'liscia' and C. medica cv. 'rugosa' are two taxa of citron, belonging to the biodiversity of South Italy, in particular of Amalfi Coast, in the Campania region. The chemical composition of the essential oils (EOs) from fruit peels of both C. medica cultivars was studied by gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS) analyses. In all, 100 compounds were identified, 82 for C. medica cv. 'liscia', accounting for 91.4% of the total oil, and 88 for C. medica cv. 'rugosa', accounting for 92.0% of the total oil. Monoterpene hydrocarbons are the main constituents in both oils of C. medica cv. 'liscia' (79.1%) and C. medica cv. 'rugosa' (80.2%). In both oils, limonene (67.2%-62.8%) and camphene (8.5%-10.9%) are the main constituents. The antimicrobial activity of the EOs was assayed against some bacterial strains: Bacillus cereus (DSM 4313), Bacillus cereus (DSM 4384), Staphylococcus aureus (DSM 25693), Pseudomonas aeruginosa (ATCC 50071), and Escherichia coli (DSM 8579). Low concentrations of C. medica cv. 'rugosa' EO showed an inhibitory effect on P. aeruginosa and higher concentrations inhibited more B. cereus (4384) and E. coli than S. aureus. The cytotoxicity of the EO was evaluated against SH-SY5Y cell line. The influence of the EO on the expression of adenylate cyclase 1 (ADCY1) was also studied. The antimicrobial activity registered confirm their traditional uses as food preserving agents and led us to hypothesize the possible use of these oils as antimicrobials. The alterations in ADCY1 expression suggested a role for limonene in effects on the central nervous system.
- Published
- 2016
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