1. Circular RNA LPAR3 sponges microRNA-198 to facilitate esophageal cancer migration, invasion, and metastasis.
- Author
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Shi Y, Fang N, Li Y, Guo Z, Jiang W, He Y, Ma Z, and Chen Y
- Subjects
- Cell Line, Tumor, Cell Movement genetics, Esophageal Neoplasms pathology, Esophageal Neoplasms surgery, Esophageal Squamous Cell Carcinoma pathology, Esophageal Squamous Cell Carcinoma secondary, Esophageal Squamous Cell Carcinoma surgery, Esophagectomy, Esophagus pathology, Esophagus surgery, Female, Gene Expression Profiling, Gene Expression Regulation, Neoplastic, Humans, Lung pathology, Lung Neoplasms secondary, Lymphatic Metastasis genetics, Male, Middle Aged, Neoplasm Invasiveness genetics, Oligonucleotide Array Sequence Analysis, Proto-Oncogene Proteins c-met genetics, RNA, Circular genetics, Receptors, Lysophosphatidic Acid genetics, Xenograft Model Antitumor Assays, Esophageal Neoplasms genetics, Esophageal Squamous Cell Carcinoma genetics, Lung Neoplasms genetics, MicroRNAs metabolism, RNA, Circular metabolism
- Abstract
In this study, we explored expression and functions of circular RNA LPAR3 (circLPAR3) in esophageal squamous cell carcinoma (ESCC). The differential expression of circular RNAs (circRNAs) in 10 ESCC and corresponding paracarcinoma tissues was analyzed through circRNA microarray, then the candidate circRNAs were detected and verified through quantitative RT-PCR, and a novel circRNA was screened, which was circLPAR3. Circular RNA LPAR3 showed apparently high expression in ESCC tissues and cells, which was closely correlated with the clinical stage and lymph node metastasis of ESCC patients. Circular RNA LPAR3 was mainly located in the cytoplasm of ESCC cells, which was more stable than the baseline gene. Circular RNA LPAR3 upregulated MET gene expression through sponge adsorption of microRNA (miR)-198, activated the RAS/MAPK and the PI3K/Akt pathways, and promoted ESCC cell migration, invasion, and metastasis in vivo and in vitro. However, it had no effect on ESCC cell proliferation. Circular RNA LPAR3 can regulate the miR-198-MET signal axis to promote the migration, invasion, and metastasis of esophageal cancer cells, which can thereby serve as a potential diagnostic and therapeutic target of esophageal cancer., (© 2020 The Authors. Cancer Science published by John Wiley & Sons Australia, Ltd on behalf of Japanese Cancer Association.)
- Published
- 2020
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