1. CpxR negatively regulates IncFII-replicon plasmid pEC011 conjugation by directly binding to multi-promoter regions.
- Author
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Jia Y, Hu H, Zhai Y, Zhao B, Sun H, Hu G, Pan Y, and Yuan L
- Subjects
- Animals, Bacterial Outer Membrane Proteins genetics, Bacterial Outer Membrane Proteins metabolism, Bacterial Proteins genetics, Base Sequence, Escherichia coli genetics, Escherichia coli metabolism, F Factor, Plasmids genetics, Promoter Regions, Genetic, Replicon, Bacterial Proteins metabolism, Escherichia coli Proteins genetics, Escherichia coli Proteins metabolism
- Abstract
CpxAR is a global regulatory protein and has important roles in plasmid mating. However, except for traJ, the regulatory effect of CpxAR on other tra genes is unclear. The aim of this study was to explore the effects of CpxAR on conjugative transfer of the epidemic plasmid pEC011 (IncFII replicon) in Escherichia coli. The plasmid mating frequencies were significantly higher for the single deletion mutant strain FΔcpxR than for the parental strain F25922. Additionally, expression levels of traM, traJ and traY in FΔcpxR were significantly higher than those in F25922. Further investigations revealed that His
6 - CpxR protein could directly bind to the traM, traJ and traY promoter regions with the binding sites of 5'-TTTACATT-3' (PM ), 5'-ATAAGAAT-3' (PJ ), and 5'-AATTTTAT-3' (PY ), respectively. Taken together, our results demonstrate that CpxAR can downregulate the expression of traM, traJ and traY by directly binding to the CpxR box-like sites of promoters, thus significantly reducing the mating rates of IncFII replicon plasmid pEC011., (Copyright © 2022 Elsevier Ltd. All rights reserved.)- Published
- 2022
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