1. Phospholipid dependency of membrane protein insertion by the Sec translocon.
- Author
-
den Uijl MJ and Driessen AJM
- Subjects
- SEC Translocation Channels metabolism, Escherichia coli genetics, Escherichia coli metabolism, Adenosine Triphosphatases metabolism, Phospholipids metabolism, Bacterial Proteins metabolism, SecA Proteins metabolism, Membrane Proteins metabolism, Escherichia coli Proteins metabolism
- Abstract
Membrane protein insertion into and translocation across the bacterial cytoplasmic membrane are essential processes facilitated by the Sec translocon. Membrane insertion occurs co-translationally whereby the ribosome nascent chain is targeted to the translocon via signal recognition particle and its receptor FtsY. The phospholipid dependence of membrane protein insertion has remained mostly unknown. Here we assessed in vitro the dependence of the SecA independent insertion of the mannitol permease MtlA into the membrane on the main phospholipid species present in Escherichia coli. We observed that insertion depends on the presence of phosphatidylglycerol and is due to the anionic nature of the polar headgroup, while insertion is stimulated by the zwitterionic phosphatidylethanolamine. We found an optimal insertion efficiency at about 30 mol% DOPG and 50 mol% DOPE which approaches the bulk membrane phospholipid composition of E. coli., Competing Interests: Declaration of competing interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Arnold J.M. Driessen reports financial support was provided by Dutch Research Council., (Copyright © 2023 The Author(s). Published by Elsevier B.V. All rights reserved.)
- Published
- 2024
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