Search

Your search keyword '"Riazankina OI"' showing total 2 results
Start Over Author "Riazankina OI" Topic escherichia coli
2 results on '"Riazankina OI"'

1. [Expression of the gene for vaccinia virus 36K nonstructural protein in Escherichia coli and study of the protective properties of expression products].

Author

Iashina LN, Gileva IP, Ryzhikov AB, Poryvaeva VA, Riazankin IA, Bondar' TS, Ovechkina LG, Riazankina OI, and Malygin EG

Subjects
Animals, Cloning, Molecular, Mice, Mice, Inbred BALB C, Vaccines, Synthetic genetics, Vaccines, Synthetic immunology, Viral Vaccines genetics, Viral Vaccines immunology, Escherichia coli genetics, Vaccinia virus genetics, Viral Proteins genetics
Abstract

The nonstructural 36K protein of vaccinia virus (VV) mapped in HindIII-P and HindIII-J fragments of VV strain L-IVP has been expressed in E. coli as a fusion protein. The products of 36K gene preserved the antigen homology with the native protein 36K and the capacity to bind specific immunoglobulins of rabbit antiVV serum. The protective properties of 36K gene products and their joint effect with the immunodominant protein 35K were investigated. The non-structural 36K gene products showed no protective activity, but increased the production of specific antibodies in mice immunized with a mixture of both protein preparations, this increase being compatible with that observed after immunization with the inactivated virus preparations.

Published
1997

2. [Expression of orthopoxvirus DNA sequences in Escherichia coli cells].

Author

Shchelkunov SN, Riazankina OI, Krykbaev RA, and Belavin PA

Subjects
Autoradiography, Base Sequence, Electrophoresis, Polyacrylamide Gel, Gene Expression, Genes, Viral, Molecular Sequence Data, Plasmids, Promoter Regions, Genetic, DNA, Viral genetics, Ectromelia virus genetics, Escherichia coli genetics, Vaccinia virus genetics
Abstract

We observed the expression of recombinant plasmids genes containing ectromelia virus DNA fragments in E. coli minicells. Using plasmids with vaccinia or ectromelia viruses DNA fragments inserted upstream of lacZ gene we showed that certain orthopoxvirus genome fragments carry out a promoter-like function in bacterial cells.

Published
1992

Catalog

Books, media, physical & digital resources
See catalog results