1. LC/MS analysis of cellular RNA reveals NAD-linked RNA.
- Author
-
Chen YG, Kowtoniuk WE, Agarwal I, Shen Y, and Liu DR
- Subjects
- Chromatography, High Pressure Liquid, Escherichia coli enzymology, Escherichia coli metabolism, Mass Spectrometry, NAD metabolism, RNA, Bacterial metabolism, RNA, Fungal metabolism, RNA, Transfer metabolism, Streptomyces enzymology, Streptomyces metabolism, Escherichia coli chemistry, NAD isolation & purification, RNA, Bacterial isolation & purification, RNA, Fungal isolation & purification, RNA, Transfer isolation & purification, Streptomyces chemistry
- Abstract
We developed a general method to detect cellular small molecule-RNA conjugates that does not rely on the reactivity of the small molecule. This technique revealed NAD-linked RNA in Escherichia coli and Streptomyces venezuelae. Subsequent characterization showed that NAD is a 5' modification of RNA, cannot be installed in vitro through aberrant transcriptional initiation, is only found among smaller cellular RNAs and is present at a surprisingly high abundance of approximately 3,000 copies per cell.
- Published
- 2009
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