1. Suppressed distribution of protein A on the surface of Staphylococcus aureus as a morphological characteristic of erythromycin-resistant strain.
- Author
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Okabe K, Chikasue K, Murakami K, Matsuda N, and Yamada S
- Subjects
- Microbial Sensitivity Tests, Enzyme-Linked Immunosorbent Assay, Polymerase Chain Reaction, Transcription, Genetic, Microscopy, Electron, Transmission, Staphylococcus aureus cytology, Staphylococcus aureus drug effects, Staphylococcus aureus metabolism, Erythromycin pharmacology, Drug Resistance, Bacterial, Bacterial Proteins metabolism, Cell Wall chemistry, Cell Wall metabolism
- Abstract
To identify a new morphological phenotype of erythromycin (EM)-resistant Staphylococcus aureus (S. aureus) were isolated in vitro from EM-sensitive parent strain, and the distribution of staphylococcus specific protein A (SpA) on the surface of these strains was examined morphologically by using applied immunoelectron microscopy. The isolated EM-resistant strains had thickened cell walls, and the distribution of SpA on the surfaces of these strains was demonstrated to be lower than that of the parent strain. The SpA suppression was confirmed by enzyme-linked immunosorbent assay (ELISA) using fixed EM-resistant cells. Moreover, the spa gene of EM-resistant cells was detected by polymerase chain reaction (PCR) and confirmed by quantitative real-time PCR assay, showing that the expression of SpA was repressed at the transcriptional level in these strains. Furthermore, ELISA assay showed that whole EM-resistant cell SpA content was significantly decreased. Therefore, it was considered that the suppression of surface SpA on the EM-resistant strain was due to regulated SpA production, and not dependent on the conformational change in SpA molecule expression through cell wall thickening. These results strongly suggest that suppressed SpA distribution on the EM-resistant S. aureus is a phenotypical characteristic in these strains., (© 2024. The Author(s) under exclusive licence to The Japanese Society for Clinical Molecular Morphology.)
- Published
- 2024
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