1. Versatile and multivalent nanobodies efficiently neutralize SARS-CoV-2
- Author
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Yufei Xiang, Dina Schneidman-Duhovny, Heng Liu, Zhe Sang, Sham Nambulli, W. Paul Duprex, Zhengyun Xiao, Cheng Zhang, and Yi Shi
- Subjects
Virus genetics ,Multidisciplinary ,biology ,Chemistry ,Protein domain ,biology.protein ,Plasma protein binding ,Antibody ,Proteomics ,Virology ,Epitope ,Aerosolization ,Neutralization - Abstract
Nanobodies that neutralize Monoclonal antibodies that bind to the spike protein of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) show therapeutic promise but must be produced in mammalian cells and need to be delivered intravenously. By contrast, single-domain antibodies called nanobodies can be produced in bacteria or yeast, and their stability may enable aerosol delivery. Two papers now report nanobodies that bind tightly to spike and efficiently neutralize SARS-CoV-2 in cells. Schoof et al. screened a yeast surface display of synthetic nanobodies and Xiang et al. screened anti-spike nanobodies produced by a llama. Both groups identified highly potent nanobodies that lock the spike protein in an inactive conformation. Multivalent constructs of selected nanobodies achieved even more potent neutralization. Science , this issue p. 1473 , p. 1479
- Published
- 2020