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Your search keyword '"Vizoso M"' showing total 3 results
Start Over Author "Vizoso M" Topic epigenesis, genetic
3 results on '"Vizoso M"'

1. Epigenetic activation of a cryptic TBC1D16 transcript enhances melanoma progression by targeting EGFR.

Author

Vizoso M, Ferreira HJ, Lopez-Serra P, Carmona FJ, Martínez-Cardús A, Girotti MR, Villanueva A, Guil S, Moutinho C, Liz J, Portela A, Heyn H, Moran S, Vidal A, Martinez-Iniesta M, Manzano JL, Fernandez-Figueras MT, Elez E, Muñoz-Couselo E, Botella-Estrada R, Berrocal A, Pontén F, Oord Jv, Gallagher WM, Frederick DT, Flaherty KT, McDermott U, Lorigan P, Marais R, and Esteller M

Subjects
Animals, Cell Line, Tumor, DNA Methylation drug effects, DNA Methylation genetics, GTPase-Activating Proteins metabolism, Immunoprecipitation, Mice, Nude, Molecular Weight, Neoplasm Metastasis, Prognosis, Promoter Regions, Genetic genetics, Protein Binding drug effects, Protein Isoforms genetics, Protein Isoforms metabolism, Protein Kinase Inhibitors pharmacology, RNA, Messenger genetics, RNA, Messenger metabolism, RNA, Small Interfering metabolism, Signal Transduction drug effects, Transcriptional Activation drug effects, Transcriptional Activation genetics, Treatment Outcome, rab GTP-Binding Proteins metabolism, Disease Progression, Epigenesis, Genetic drug effects, ErbB Receptors metabolism, GTPase-Activating Proteins genetics, Melanoma genetics, Melanoma pathology
Abstract

Metastasis is responsible for most cancer-related deaths, and, among common tumor types, melanoma is one with great potential to metastasize. Here we study the contribution of epigenetic changes to the dissemination process by analyzing the changes that occur at the DNA methylation level between primary cancer cells and metastases. We found a hypomethylation event that reactivates a cryptic transcript of the Rab GTPase activating protein TBC1D16 (TBC1D16-47 kDa; referred to hereafter as TBC1D16-47KD) to be a characteristic feature of the metastatic cascade. This short isoform of TBC1D16 exacerbates melanoma growth and metastasis both in vitro and in vivo. By combining immunoprecipitation and mass spectrometry, we identified RAB5C as a new TBC1D16 target and showed that it regulates EGFR in melanoma cells. We also found that epigenetic reactivation of TBC1D16-47KD is associated with poor clinical outcome in melanoma, while conferring greater sensitivity to BRAF and MEK inhibitors.

Published
2015
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3. German-Catalan workshop on epigenetics and cancer.

Author

Vizoso M and Esteller M

Subjects
Antineoplastic Agents pharmacology, Antineoplastic Agents therapeutic use, Chromatin genetics, Chromatin metabolism, Epigenomics methods, Histones metabolism, Humans, Neoplasms drug therapy, Prognosis, DNA Methylation, Epigenesis, Genetic, Histones genetics, Neoplasms genetics, RNA, Untranslated genetics
Abstract

In the First German-Catalan Workshop on Epigenetics and Cancer held in Heidelberg, Germany (June 17-19, 2013), cutting-edge laboratories (PEBC, IMPPC, DKFZ, and the Collaborative Research Centre Medical Epigenetics of Freiburg) discussed the latest breakthroughs in the field. The importance of DNA demethylation, non-coding and imprinted genes, metabolic stress, and cell transdifferentiation processes in cancer and non-cancer diseases were addressed in several lectures in a very participative and dynamic atmosphere.   The meeting brought together leading figures in the field of cancer epigenetics to present their research work from the last five years. Experts in different areas of oncology described important advances in colorectal, lung, neuroblastoma, leukemia, and lymphoma cancers. The workshop also provided an interesting forum for pediatrics, and focused on the need to improve the treatment of childhood tumors in order to avoid, as far as possible, brain damage and disruption of activity in areas of high plasticity. From the beginning, the relevance of "omics" and the advances in genome-wide analysis platforms, which allow cancer to be studied in a more comprehensive and inclusive way, was very clear. Modern "omics" offer the possibility of identifying metastases of uncertain origin and establishing epigenetic signatures linked to a specific cluster of patients with a particular prognosis. In this context, invited speakers described novel tumor-associated histone variants and DNA-specific methylation, highlighting their close connection with other processes such as cell-lineage commitment and stemness.

Published
2013
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