6 results on '"Tao, Lin"'
Search Results
2. Declined expressing mRNA of beta-defensin 108 from epididymis is associated with decreased sperm motility in blue fox (Vulpes lagopus)
- Author
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Wu, Ping, Liu, Tao-lin, Li, Ling-ling, Liu, Zhi-ping, Tian, Li-hong, and Hou, Zhi-jun
- Published
- 2021
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3. Declined expressing mRNA of beta-defensin 108 from epididymis is associated with decreased sperm motility in blue fox (Vulpes lagopus)
- Author
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Li-hong Tian, Ping Wu, Zhijun Hou, Ling-ling Li, Tao-lin Liu, and Zhi-ping Liu
- Subjects
Male ,beta-Defensins ,Vulpes ,animal diseases ,Foxes ,Pathogenesis ,Andrology ,03 medical and health sciences ,Vulpes lagopus ,0302 clinical medicine ,parasitic diseases ,medicine ,Animals ,RNA, Messenger ,Animal Husbandry ,Sperm motility ,030304 developmental biology ,Epididymis ,0303 health sciences ,Messenger RNA ,lcsh:Veterinary medicine ,030219 obstetrics & reproductive medicine ,General Veterinary ,biology ,virus diseases ,food and beverages ,General Medicine ,biology.organism_classification ,Fertility ,medicine.anatomical_structure ,Beta defensin ,Asthenozoospermia ,asthenospermia ,Sperm Motility ,biology.protein ,lcsh:SF600-1100 ,population characteristics ,Immunohistochemistry ,Antibody ,vBD108 ,Research Article - Abstract
Background Fecundity is important for farm blue fox (Vulpes lagopus), who with asthenospermia have be a problem in some of farms in China. A key symptom of asthenospermia is decreased sperm motility. The decreased secreting beta-defensin108 (vBD108) of blue fox is speculated be related to asthenospermia. To clarify this idea, the mRNA expression of vBD108 in testis and epididymis of blue foxes with asthenospermia were detected and compared to the healthy one. The antibody was prepared and analyzed by immunohistochemistry. Results The vBD108 in testis and epididymis was found both in blue fox with asthenospermia and healthy group by the method of immunohistochemistry. The expression of vBD108 mRNA in testes (P P Conclusions These results suggested that vBD108 deficiency may related to blue fox asthenospermia. Meanwhile, the study on the blue fox vBD108 provides a hopeful direction to explore the pathogenesis of blue fox asthenospermia in the future.
- Published
- 2021
4. Urban fine particulate matter exposure causes male reproductive injury through destroying blood-testis barrier (BTB) integrity
- Author
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Bo Liu, Yue Zhou, Shengde Wu, Geng Xiong, Yang Liu, Xiangliang Tang, Chao Yan, Yangcai Wang, Dong-yao Liu, Guanghui Wei, Lianju Shen, Tao Lin, Dawei He, Mang Sun, Chunlan Long, Min Guo, and Xining Cao
- Subjects
0301 basic medicine ,Male ,Apoptosis ,010501 environmental sciences ,Biology ,Toxicology ,01 natural sciences ,Male infertility ,Andrology ,Adherens junction ,Rats, Sprague-Dawley ,03 medical and health sciences ,medicine ,Animals ,Blood-Testis Barrier ,Cells, Cultured ,Infertility, Male ,0105 earth and related environmental sciences ,Blood–testis barrier ,chemistry.chemical_classification ,Epididymis ,Reactive oxygen species ,Sertoli Cells ,Tight junction ,General Medicine ,medicine.disease ,Sertoli cell ,Immunohistochemistry ,Rats ,030104 developmental biology ,medicine.anatomical_structure ,chemistry ,Immunology ,Particulate Matter ,Spermatogenesis - Abstract
Blood-testis barrier (BTB) provides a suitable microenvironment for germ cells that is required for spermatogenesis. Exposure to particulate matter (PM) is recognized to occasion male reproductive impairment, but the mechanism of which remains unclear. Male Sprague-Dawley (SD) rats were used to establish animal models with PM2.5 exposure concentration of 0, 10, and 20mg/kg.b.w. once a day for four weeks. Success rate of mating, sperm quality, epididymal morphology, expressions of spermatogenesis markers, superoxide dismutases (SOD) activity and expression in testicular tissues, and expressions of BTB junction proteins were detected. In addition, in vitro experiments were also performed. After PM2.5 treatment, reactive oxygen species (ROS) production and apoptosis of Sertoli cells were analyzed. Our results indicated that after PM2.5 exposure male rats presented inferior uberty and sperm quality, with decreased expressions of spermatogenesis markers, escalated SOD activity and expression levels, and reduced expressions of tight junction, adherens junction, and gap junction proteins in testicular tissues. Meantime, PM2.5-treated Sertoli cells displayed increased SOD production and apoptosis. PM2.5 exposure engenders male reproductive function injury through breaking BTB integrity.
- Published
- 2016
5. The Mechanism of Environmental Endocrine Disruptors (DEHP) Induces Epigenetic Transgenerational Inheritance of Cryptorchidism
- Author
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Tao Lin, Lianju Shen, Sheng Wen, Jinpu Peng, Yue Zhou, Chunlan Long, Shengde Wu, Yi Hua, Guanghui Wei, Xining Cao, Jin-jun Chen, Chao Yan, and Dawei He
- Subjects
medicine.medical_specialty ,Pregnancy ,Multidisciplinary ,Offspring ,Science ,Biology ,Epididymis ,medicine.disease ,Sperm ,medicine.anatomical_structure ,Endocrinology ,Internal medicine ,DNA methylation ,medicine ,Medicine ,Reproductive system ,Epigenetics ,Genomic imprinting ,Research Article - Abstract
Discussion on the role of DEHP in the critical period of gonadal development in pregnant rats (F0), studied the evolution of F1-F4 generation of inter-generational inheritance of cryptorchidism and the alteration of DNA methylation levels in testis. Pregnant SD rats were randomly divided into two groups: normal control group and DEHP experimental group. From pregnancy 7 d to 19 d, experimental group was sustained to gavage DEHP 750 mg/kg bw/day, observed the incidence of cryptorchidism in offspring and examined the pregnancy rate of female rats through mating experiments. Continuous recording the rat's weight and AGD value, after maturation (PND80) recording testis and epididymis' size and weight, detected the sperm number and quality. Subsequently, we examined the evolution morphological changes of testicular tissue for 4 generation rats by HE staining and Western Blot. Completed the MeDIP-sequencing analysis of 6 samples (F1 generation, F4 generation and Control). DEHP successfully induced cryptorchidism occurrence in offspring during pregnancy. The incidence of cryptorchidism in F1 was 30%, in F2 was 12.5%, and there was no cryptorchidism coming up in F3 and F4. Mating experiment shows conception rate 50% in F1, F2 generation was 75%, the F3 and F4 generation were 100%. HE staining showed that the seminiferous epithelium of F1 generation was atrophy and with a few spermatogenic cell, F2 generation had improved, F3 and F4 generation were tend to be normal. The DNA methyltransferase expression was up-regulated with the increase of generations by Real Time-PCR, immunohistochemistry and Western Blot. MeDIP-seq Data Analysis Results show many differentially methylated DNA sequences between F1 and F4. DEHP damage male reproductive function in rats, affect expression of DNA methyltransferase enzyme, which in turn leads to genomic imprinting methylation pattern changes and passed on to the next generation, so that the offspring of male reproductive system critical role in the development of imprinted genes imbalances, and eventually lead to producing offspring cryptorchidism. This may be an important mechanism of reproductive system damage.
- Published
- 2015
6. The Mechanism of Environmental Endocrine Disruptors (DEHP) Induces Epigenetic Transgenerational Inheritance of Cryptorchidism
- Author
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Jinjun Chen, Shengde Wu, Sheng Wen, Lianju Shen, Jinpu Peng, Chao Yan, Xining Cao, Yue Zhou, Chunlan Long, Tao Lin, Dawei He, Yi Hua, and Guanghui Wei
- Subjects
DNA (Cytosine-5-)-Methyltransferase 1 ,Epididymis ,Male ,Multidisciplinary ,Sperm Count ,lcsh:R ,lcsh:Medicine ,Correction ,DNA Methylation ,Endocrine Disruptors ,Epigenesis, Genetic ,Rats, Sprague-Dawley ,Pregnancy ,Diethylhexyl Phthalate ,Prenatal Exposure Delayed Effects ,Cryptorchidism ,Testis ,Animals ,lcsh:Q ,Female ,DNA (Cytosine-5-)-Methyltransferases ,lcsh:Science - Abstract
Discussion on the role of DEHP in the critical period of gonadal development in pregnant rats (F0), studied the evolution of F1-F4 generation of inter-generational inheritance of cryptorchidism and the alteration of DNA methylation levels in testis. Pregnant SD rats were randomly divided into two groups: normal control group and DEHP experimental group. From pregnancy 7 d to 19 d, experimental group was sustained to gavage DEHP 750 mg/kg bw/day, observed the incidence of cryptorchidism in offspring and examined the pregnancy rate of female rats through mating experiments. Continuous recording the rat's weight and AGD value, after maturation (PND80) recording testis and epididymis' size and weight, detected the sperm number and quality. Subsequently, we examined the evolution morphological changes of testicular tissue for 4 generation rats by HE staining and Western Blot. Completed the MeDIP-sequencing analysis of 6 samples (F1 generation, F4 generation and Control). DEHP successfully induced cryptorchidism occurrence in offspring during pregnancy. The incidence of cryptorchidism in F1 was 30%, in F2 was 12.5%, and there was no cryptorchidism coming up in F3 and F4. Mating experiment shows conception rate 50% in F1, F2 generation was 75%, the F3 and F4 generation were 100%. HE staining showed that the seminiferous epithelium of F1 generation was atrophy and with a few spermatogenic cell, F2 generation had improved, F3 and F4 generation were tend to be normal. The DNA methyltransferase expression was up-regulated with the increase of generations by Real Time-PCR, immunohistochemistry and Western Blot. MeDIP-seq Data Analysis Results show many differentially methylated DNA sequences between F1 and F4. DEHP damage male reproductive function in rats, affect expression of DNA methyltransferase enzyme, which in turn leads to genomic imprinting methylation pattern changes and passed on to the next generation, so that the offspring of male reproductive system critical role in the development of imprinted genes imbalances, and eventually lead to producing offspring cryptorchidism. This may be an important mechanism of reproductive system damage.
- Published
- 2015
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