1. Single-keratinocyte transcriptomic analyses identify different clonal types and proliferative potential mediated by FOXM1 in human epidermal stem cells.
- Author
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Enzo E, Secone Seconetti A, Forcato M, Tenedini E, Polito MP, Sala I, Carulli S, Contin R, Peano C, Tagliafico E, Bicciato S, Bondanza S, and De Luca M
- Subjects
- Adaptor Proteins, Signal Transducing metabolism, Animals, Cell Adhesion genetics, Cell Line, Cell Self Renewal genetics, Cells, Cultured, Chromatin Immunoprecipitation, Epidermal Cells metabolism, Epidermolysis Bullosa, Junctional genetics, Epidermolysis Bullosa, Junctional metabolism, Forkhead Box Protein M1 genetics, Gene Expression Profiling, Gene Ontology, Humans, Keratinocytes metabolism, Mice, Microarray Analysis, Multigene Family, RNA-Seq, Stem Cells metabolism, Transcription Factors metabolism, YAP-Signaling Proteins, Epidermal Cells cytology, Forkhead Box Protein M1 metabolism, Keratinocytes cytology, Single-Cell Analysis methods, Stem Cells cytology, Transcriptome genetics
- Abstract
Autologous epidermal cultures restore a functional epidermis on burned patients. Transgenic epidermal grafts do so also in genetic skin diseases such as Junctional Epidermolysis Bullosa. Clinical success strictly requires an adequate number of epidermal stem cells, detected as holoclone-forming cells, which can be only partially distinguished from the other clonogenic keratinocytes and cannot be prospectively isolated. Here we report that single-cell transcriptome analysis of primary human epidermal cultures identifies categories of genes clearly distinguishing the different keratinocyte clonal types, which are hierarchically organized along a continuous, mainly linear trajectory showing that stem cells sequentially generate progenitors producing terminally differentiated cells. Holoclone-forming cells display stem cell hallmarks as genes regulating DNA repair, chromosome segregation, spindle organization and telomerase activity. Finally, we identify FOXM1 as a YAP-dependent key regulator of epidermal stem cells. These findings improve criteria for measuring stem cells in epidermal cultures, which is an essential feature of the graft.
- Published
- 2021
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