Kaur, Harry, Rode, Surabhi, KP, Sandra, Mahto, Jai Krishna, Alam, Md Shahid, Gupta, Deena Nath, Kar, Bibekananda, Singla, Jitin, Kumar, Pravindra, and Sharma, Ashwani Kumar
The haloacid dehalogenase superfamily implicated in bacterial pathogenesis comprises different enzymes having roles in many metabolic pathways. Staphylococcus lugdunensis , a Gram-positive bacterium, is an opportunistic human pathogen causing infections in the central nervous system, urinary tract, bones, peritoneum, systemic conditions and cutaneous infection. The haloacid dehalogenase superfamily proteins play a significant role in the pathogenicity of certain bacteria, facilitating invasion, survival, and proliferation within host cells. The genome of S. lugdunensis encodes more than ten proteins belonging to this superfamily. However, none of them have been characterized. The present work reports the characterization of one of the haloacid dehalogenase superfamily proteins (SLHAD1) from Staphylococcus lugdunensis. The functional analysis revealed that SLHAD1 is a metal-dependent acid phosphatase, which catalyzes the dephosphorylation of phosphorylated metabolites of cellular pathways, including glycolysis, gluconeogenesis, nucleotides, and thiamine metabolism. Based on the substrate specificity and genomic analysis, the physiological function of SLHAD1 in thiamine metabolism has been tentatively assigned. The crystal structure of SLHAD1, lacking 49 residues at the C -terminal, was determined at 1.7 Å resolution with a homodimer in the asymmetric unit. It was observed that SLHAD1 exhibited time-dependent cleavage at a specific point, occurring through a self-initiated process. A combination of bioinformatics, biochemical, biophysical, and structural studies explored unique features of SLHAD1. Overall, the study revealed a detailed characterization of a critical enzyme of the human pathogen Staphylococcus lugdunensis , associated with several life-threatening infections. [Display omitted] • HAD family acid phosphatase was purified and characterized from S. lugdunensis. • It showed broad substrate specificity and a significant role in thiamine metabolism. • First report of time-dependent self-cleaving property of HAD family proteins. • The crystal structure of a truncated version was analyzed. • Removal of 49 residues from the C -terminal leads to protein inactivation. [ABSTRACT FROM AUTHOR]