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In the laboratory-scale experiment effects of the wastes containing oil components and elements of natural radioactive families U238, Th232 and K40, on a microbial biomass, soil respiration, metabolic quotient, dehydrogenase activity and number of bacteria of genus Azotobacter of gray forest soil is studied.

For estimation of quality of soil, degradated by water erosion, the integral indicator based on assessment of humus layer thickness, slope angle, soil organic matter content, microbial biomass and plants characteristics is suggested. The use of this indicator permits to reveal the differences in erosion level of soils which have the equal thickness of humus layer but are situated on different parts of slope.

The effects of metals (Сu, Pb, Ni, Cr, Cd) and fungicide alto-super on dehydrogenase activities of indigenous microflora of grey forest soil and laboratory culture Bacillus pumilus КМ-21 were studied. It was determined that the putting of the metals into soil caused the dose-effect inhibition of dehydrogenase activities of indigenous microflora of soil and dehydrogenase activities Bacillus pumilus КМ-21 estimated in eluate and contact tests. On the base of the established metal concentration causing the 50% inhibition on dehydrogenase activity, it was revealed that the contact test was more sensitive in comparison with eluate test. Cose relation between the alteration in activities of microbial culture (contact test) and indigenous microflora was shown. It allowed to recommend the contact test for estimation of the soil contamination with toxic compounds.