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Nitrogen removal is a critical process in water treatment plants (WTPs) and wastewater treatment plants (WWTPs). The recent discovery of a novel bacterial process, complete ammonia oxidation (comammox, CMX), has refuted a century-long perception of the two-step conversion of NH3 to NO3–. Compared with canonical nitrifiers, CMX bacteria offer undeniable advantages, such as a high growth yield propensity and adaptability to nutrient- and growth-limiting conditions, which collectively draw attention to validate the aptness of CMX bacteria to wastewater treatment. As there has been no comprehensive review on the relevance of CMX bacteria for sustainable water and wastewater treatment, this review is intended to discuss the roles and applications of CMX in the removal of nitrogen and pollutants from water and wastewater. We took into account insights into the metabolic versatilities of CMX bacteria at the clade and subclade levels. We focused on the distribution of CMX bacteria in engineered systems, niche differentiation, co-occurrence and interactions with canonical nitrifiers for a better understanding of CMX bacteria in terms of their ecophysiology. Conceptualized details on the reactor adaptability and stress response of CMX bacteria are provided. The potential of CMX bacteria to degrade micropollutants either directly or co-metabolically was evaluated, and these insights would be an indispensable advantage in opening the doors for wider applications of CMX bacteria in WWTPs. Finally, we summarized future directions of research that are imperative in improving the understanding of CMX biology.

A combined system consisting of an upflow blanket filter (UBF) and a moving-bed biofilm reactor (MBBR) was developed for the simultaneous removal of organic matters and ammonia from high-strength wastewater. With a constant COD of approximately 2000 mg/L and ammonium nitrogen in a series of concentrations (e.g., 50, 200 and 400 mg/L in stages I to III) of the influent wastewater, the removal efficiencies of COD, ammonium nitrogen and total nitrogen reached 96.10%-98.19%, 100%, and 79.12%-82.15%, respectively. With the increase of influent ammonia nitrogen concentration, the specific methanogenic activity of the UBF granules decreased significantly, while the specific denitrification rates of the UBF granules and specific nitrification rates of the MBBR biofilms increased significantly. Microbial community analysis showed that Methanobacterium and Methanosaeta were the dominant methanogens in the UBF granules, while Candidatus Competibacter, Thauera and Acinetobacter were identified as dominant denitrifiers. In addition, nitrifiers were enriched in MBBR biofilms at 11.33% and 13.87% of the average abundance of Nitrosomonas and Nitrospira, respectively, at stage III (influent ammonium at 400 mg/L, COD/NH

Microbial immigrants arriving with influent wastewater may influence activated sludge (AS) ecosystems. However, the extent to which immigration impacts AS communities is still debated. To explore the intensity of immigration impact, we used sequencing technology to track the raw wastewater and AS communities from a membrane bioreactor plant over a 12-month period. We first distinguished core populations from peripheral ones in both raw wastewater and AS based on their occurrence frequency and abundance. The results showed that core OTUs (≥ 80% occurrence frequency) made up a large fraction (> 90%) of total sequences, while peripheral OTUs composed the majority of all detected OTUs but merely occupied a few sequences. A significant difference in core communities between the influent and AS was found, as well as between the compositions of core and peripheral populations. Additionally, the persistent functional bacteria of AS, although not numerically dominant, accounted for 96.24% of the total sequences related to nutrient turnover, suggesting the presence of a small number of longstanding and core functional bacteria in the AS ecosystem. Importantly, 64% of the 5188 OTUs in AS, which accounted for 91.51% of the sequences, exhibited positive growth rates, which suggested that their apparent abundances were due to growth within the plant, not from immigration. Taken together, these results demonstrated that the impact of influent populations on core AS communities was limited. Overall, this work provides quantitative insights into the impact of immigration, which is expected to advance our understanding of the AS community assembly.

The quality and quantity of domestic sewage discharge vary significantly during the Chinese Spring Festival due to the huge population shift. The dynamics of microbial community traits during the Spring Festival, particularly the phylogenetic group-based assembly and co-occurrence patterns, are however little understood. Here, influent and activated sludge samples from 2 full-scale wastewater treatment plants were collected bi-daily throughout a 20-day Spring Festival period and subjected to high-throughput Illumina-MiSeq sequencing. The findings revealed that whereas the microbial communities in the activated sludge displayed a comparatively stable pattern, and the influent communities experienced significant temporal fluctuations in terms of diversity and composition. The characterization by "Infer Community Assembly Mechanisms by Phylogenetic-bin based null model" demonstrated that for Competibacter glycogen-accumulating organisms, the assembly mechanism shifted from deterministic process (HoS = 69.5%) before the Spring Festival to stochastic process (DR = 65.9%) after the Spring Festival. The network analysis revealed that the network structure of sludge communities was more stable before the Spring Festival than that after the Spring Festival. Additionally, sludge communities had no keystone species in common with the influent before the Spring Festival, while the sludge and influent communities shared two keystone taxa after the Spring Festival (Sebaldella and Candidatus Competibacter). This study would deepen our understanding of the microbial ecology in biological wastewater treatment systems, which also aids in managing wastewater treatment plants.

In the face of the growing global water crisis, membrane technology is a promising means of purifying water and wastewater. Silver nanoparticles (AgNPs) have been widely used to improve membrane performance, for antibiofouling, and to aid in photocatalytic degradation, thermal response, and electro-conductivity. However, several critical issues such as short antimicrobial periods, trade-off effects and silver inactivation seriously restrict the engineering application of AgNPs-incorporated membranes. In addition, there is controversy around the use of AgNPs given the toxic preparation process and environmental/biological risks. Hence, it is of great significance to summarize and analyze the recent developments and critical challenges in the use of AgNPs-incorporated membranes in water and wastewater treatment, and to propose potential solutions. We reviewed the different properties and functions of AgNPs and their corresponding applications in AgNPs-incorporated membranes. Recently, multifunctional, novel AgNP-incorporated membranes combined with other functional materials have been developed with high-performance. We further clarified the synergistic mechanisms between AgNPs and these novel nanomaterials and/or polymers, and elucidated their functions and roles in membrane separation. Finally, the critical challenges of AgNPs-incorporated membranes and the proposed solutions were outlined: i) Prolonging the antimicrobial cycle through long-term and controlled AgNPs release; ii) Overcoming the trade-off effect and organic fouling of the AgNPs-incorporated membranes; iii) Preparation of sustainable AgNPs-incorporated membranes; iv) Addressing biotoxicity induced by AgNPs; and v) Deactivation of AgNPs-incorporated membrane. Overall, this review provides a comprehensive discussion of the advancements and challenges of AgNPs-incorporated membranes and guides the development of more robust, multi-functional and sustainable AgNPs-incorporated membranes.

Adaptation to substrate fluctuations is a life actuality of microbes in global municipal wastewater treatment plants (WWTPs). Yet there remains a lack of definite information on how influent changes with different alternation frequencies shape the stability of anammox consortia and the metabolic regulations they feedback. According to human rhythmic activity, day-fluctuant fed (every 6 h, alternating between 50 and 100 mg NH

Fouling mechanism of the forward osmosis membrane, which was peculiarly featured by the asymmetric membrane structure and reverse solute diffusion, was investigated at the molecular level and from the energy perspective. Two noteworthy fouling behaviors were observed in batch fouling tests conducted in AL-FS mode (active layer facing feed solution) and AL-DS mode (active layer facing draw solution) after filtering foulants with identical volume: 1) after filtering 100 mL of foulants, the flux decline rate in AL-DS mode was 1.78 times faster than that in AL-FS mode, but the flux decline behaviors of the two modes were similar in the subsequent filtration stages; 2) although the foulant layer weight of the same mode increased linearly in middle and late stages, the flux loss rate was distinctly different. Thermodynamic analysis indicated that the attractive interaction energy between the foulants and the support layer was about 5 times higher than that between the foulants and the active layer, well interpreting the higher flux decline rate of AL-DS mode in initial stage. Meanwhile, a non-invasive microscope observed that the structure of the fouling layer remarkably changed from loose to dense in the middle stage, and stabilized in the late stage. Furthermore, quantum chemistry calculation proved that the reverse diffusion of NaCl brought alginate molecular chains closer, whereas the distance between them tended to be constant as the continuous increase of NaCl. Based on these findings, the thermodynamic fouling mechanism proposed by combining the structure change process of the fouling layer with Flory-Huggins lattice theory satisfactorily interpreted the noteworthy fouling behaviors caused by reverse NaCl diffusion in middle and late stages. The revealed fouling mechanism unifies the adhesion and filtration behaviors related to the unique properties of FO membrane, deepening understanding of membrane fouling in the dynamic and complex ternary system of the FO process.

Denitrifying bacteria with high abundances in anammox communities play crucial roles in achieving stable anammox-based systems. Despite the relative constant composition of denitrifying bacteria, their functional diversity remains to be explored in anammox communities. Herein, a total of 77 high-quality metagenome-assembled genomes (MAGs) of denitrifying bacteria were recovered from the anammox community in a full-scale swine wastewater treatment plant. Among these microbes, a total of 26 MAGs were affiliated with the seven dominant denitrifying genera that have total abundances higher than 1%. A meta-analysis of these species suggested that external organics reduced the abundances of genus Ignavibacterium and species MAG.305 of UTPRO2 in anammox communities. Comparative genome analysis revealed functional divergence across different denitrifying bacteria, largely owing to their distinct capabilities for carbohydrate (including endogenous and exogenous) utilization and vitamin (e.g., pantothenate and thiamine) biosynthesis. Serval microbes in this system contained fewer genes encoding biotin, pantothenate and methionine biosynthesis compared with their related species from other habitats. In addition, the genes encoding energy production and conversion (73 genes) and inorganic ion transport (53 genes) putatively transferred from other species to denitrifying bacteria, while these denitrifying bacteria (especially genera UTPRO2 and SCN-69-89) likely donated the genes encoding nutrients (e.g., inorganic ion and amino acid) transporter (64 genes) for other members to utilize new metabolites. Collectively, these findings highlighted the functional divergence of these denitrifying bacteria and speculated that the genetic interactions within anammox communities through horizontal gene transfer may be one of the reasons for their functional divergence.

Sub-visible particles, an often-overlooked fine particle (0.45–10 μm) with a size between sludge solids and soluble microbial products (SMP), have recently been identified as a critical foulant in anaerobic membrane bioreactors (AnMBRs), and our recent new insights into the size-fractionation and composition of sub-visible particles in AnMBRs have enabled fouling to be understood in more depth. Here, we investigated the microbial diversity of the sub-visible particles in three size fractions (i.e., 5–10, 1–5, and 0.45–1 μm) from bulk and cake solutions in a lab-scale AnMBR, and their fouling potential was further explored based on their filtration behavior and biofilm formation. Results show that with decreasing particle size, a significant shift in microbial communities was observed for the sub-visible particles in both bulk and cake solutions; (a) with notable decreases in filamentous microbes in the order SJA-15, GCA004, and Anaerolineales of phylum Chloroflexi, and, (b) with substantial increases in sulfate-reducing bacteria (i.e., the family Syntrophobacteraceae, genus DCE29 of family Thermodesulfovibrionaceae, Desulfovibrio, and Geobacter). More importantly, the filamentous microbes associated with micro-particles (5–10 μm) led to higher cake fouling resistances while free living cells in the form of colloidal particles (0.45–1 μm) induced severer pore blocking. Moreover, the micro-particles had an enhanced capacity to favor biofilm formation (OD595 = 1.0–2.5, categorized as highly positive), thus potentially aggravating biofouling. This work advances our knowledge on the effect of particle size on communities and underlying fouling behavior of microbes associated with fine particles in AnMBRs. Environment & Water Industry Development Council (EWI) This work was funded by the Environmental & Water Industry Programme Office (PUB IDD 21100/36/6) in Singapore. It was also supported by the National Natural Science Foundation of China (No. 51608546) and the Fundamental Research Funds for the Central Universities (No. 17lgpy94). We are grateful to Dr. Guangyi Su for support with community analysis.

Much about assembly processes dictating bio-cake microbiota remains uncertain, leading to poor understanding of membrane biofouling in membrane bioreactors (MBRs). This work aimed to reveal the underlying mechanisms driving bio-cake community during the biofouling process under different flux conditions. On the basis of 16S rRNA sequences, the results showed that bacterial diversity decreased with increasing fouling. Additionally, low-flux bio-cake (8 LMH) communities harbored much lower diversity than high-flux (16 LMH) bio-cake microbiomes. Ecological null model analyses and phylogenetic molecular ecological networks (pMENs) revealed that environmental filtering deterministically governed low-flux bio-cake communities. In contrast, high-flux bio-cake communities were mainly shaped in a stochastic manner. This is likely due to the higher stochastic deposition of bacterial taxa from bulk sludge because of the presence of a stronger drag force. Moreover, by lowering the flux, the interactions between bacterial lineages were enhanced; this is evidenced by the greater number of links, the higher average degree, and the higher average clustering coefficients within the pMENs in low-flux bio-cakes than those in high-flux bio-cakes. Most keystone fouling-related taxa in low-flux bio-cakes were motile and involved in nitrate reduction and polysaccharide/protein metabolism. This corroborated the important role of environmental filtering in the assembly process dictating low-flux bio-cake formation. Some low-abundance taxa were observed to be key fouling-related bacteria under both flux conditions, indicating that a few populations play paramount ecological roles in triggering biofouling. In summary, our findings clearly indicate distinct bio-cake community assembly patterns under different operational conditions and highlight the importance of developing specialized strategies for fouling control in individual MBR systems.

While there has been a significant recent improvement in the removal of pollutants in natural and engineered systems, trace organic chemicals (TrOCs) are posing a major threat to aquatic environments and human health. There is a critical need for developing potential strategies that aim at enhancing metabolism and/or cometabolism of these compounds. Recently, knowledge regarding biodegradation of TrOCs by ammonia-oxidizing bacteria (AOB) has been widely developed. This review aims to delineate an up-to-date version of the ecophysiology of AOB and outline current knowledge related to biodegradation efficiencies of the frequently reported TrOCs by AOB. The paper also provides an insight into biodegradation pathways by AOB and transformation products of these compounds and makes recommendations for future research of AOB. In brief, nitrifying WWTFs (wastewater treatment facilities) were superior in degrading most TrOCs than non-nitrifying WWTFs due to cometabolic biodegradation by the AOB. To fully understand and/or enhance the cometabolic biodegradation of TrOCs by AOB, recent molecular research has focused on numerous crucial factors including availability of the compounds to AOB, presence of growth substrate (NH4-N), redox potentials, microorganism diversity (AOB and heterotrophs), physicochemical properties and operational parameters of the WWTFs, molecular structure of target TrOCs and membrane-based technologies, may all significantly impact the cometabolic biodegradation of TrOCs. Still, further exploration is required to elucidate the mechanisms involved in biodegradation of TrOCs by AOB and the toxicity levels of formed products.

The dissolved organic matter (DOM) characterization in a full-scale landfill leachate treatment plant is of great importance for the design and operation of treatment processes. In this study, the long-term removal behaviors of DOM during landfill leachate treatment were explored using excitation emission matrix fluorescence spectroscopy (EEMs) coupled with parallel factor analysis (PARAFAC) and self-organizing map (SOM). Results indicated that the application of combining PARAFAC and SOM on EEMs analysis effectively characterized long-term removal behaviors of DOM during leachate treatment. The DOM in raw leachate was dominated by humic substances, while its composition exhibited significant seasonal differences. A large proportion of protein-like fluorescent dissolved organic matter (FDOM) and bulk DOM were removed within membrane bioreactor (MBR) system. Meanwhile the humic-like FDOM removal capacity in nanofiltration (NF) process was well comparable with those in the MBR system owing to the bio-recalcitrant nature of humic substances. The protein-like FDOM and bulk DOM were removed synchronously in both the process of MBR and NF. Moreover, samples distribution exhibited obvious differences among NF concentrate samples. In general, the performance of MBR-NF treatment for landfill leachate displayed reasonable stability in DOM removal irrespective of seasonal variations. This study enhanced our understanding of EEMs application in characterizing leachate-derived DOM composition and has potential implications for the associated monitoring investigations in engineered systems.

In this pilot-scale study, a flow-through biofilm reactor (FTBR) was investigated for municipal wastewater treatment. The removal efficiencies for ammonium, total nitrogen, total phosphorus, and chemical oxygen demand were 87.2 ± 17.9%, 61.1 ± 13.9%, 83.5 ± 11.9%, and 92.6 ± 1.7%, respectively, at low dissolved oxygen concentrations (averaged at 0.59 mg/L), indicating the feasibility and robustness of the FTBR for a simultaneous nitrification, denitrification, and phosphorous removal (SNDPR) process. The co-occurrence network of bacteria in the dynamic biofilm was complex, with equivalent bacterial cooperation and competition. Nevertheless, the bacterial interactions in the suspended sludge were mainly cooperative. The presence of dynamic biofilms increased bacterial diversity by creating niche differentiation, which enriched keystone species closely related to nutrient removal. Overall, this study provides a novel FTBR-based SNDPR process and reveals the ecological mechanisms responsible for nutrient removal.

This study aimed to better understand the transformation behaviors of extracellular polymeric substances (EPS) and their roles in regulating bacterial community in biological wastewater treatment processes. Herein, well-controlled bioassays under aerobic and anoxic conditions were performed to investigate degradation dynamics, composition variations, and bacterial response during EPS transformation. Reactivity continuum modeling showed that organic pools of EPS had continuous reactivity distributions, and most labile organic fraction with a degrading rate0.1 h

Understanding the temporal dynamics and recovery of anammox community under nitrite stress is critical for successful application of anammox-related processes. Here, the response behaviors of anammox community were investigated to characterize the reactor performance and ecological function under varied levels of nitrite stress (changing from 0, 50, 100, 200 to 0 mg-N/L) across a large temporal scale (588 days). The nitrogen removal rates decreased from 0.51 ± 0.02 to 0.16 ± 0.04 kg-N/(m

The complexity of production process and chemical compositions of soluble microbial products (SMPs) largely limits the understanding of membrane fouling in membrane bioreactors (MBRs). Herein, we used a model single-strain Escherichia coli to better understand the chemical natures of SMPs and their roles in membrane fouling. The effects of carbon source and growth phase on the chemical compositions of SMPs were identified at both the compound and molecular levels by using advanced techniques including excitation emission matrix and parallel factor analysis (EEM-PARAFAC), size exclusion chromatography coupled with organic carbon detection (LC-OCD), and untargeted ultra-performance liquid chromatography - Q-Exactive - mass spectrometry (UPLC-Q-Exactive-MS). Subsequently, the roles of SMPs in the propensity of membrane fouling during ultrafiltration (UF) were studied. The results showed that the chemical compositions and fouling potentials of SMPs were carbon source- and growth phase-dependent. In the exponential phase, SMPs mainly consisted of utilization-associated products (UAPs) and remaining substrates. As the microorganism progressed into the stationary and senescent phases, UAPs and biomass-associated products (BAPs) were the main components, respectively. The SMP contents generated in glucose medium were higher than those generated in acetate medium, and higher abundances of humic fluorescent components were observed in glucose-fed SMPs. Van Krevelen diagrams of the UPLC-MS results revealed that acetate-fed SMPs contained more carboxylic-rich alicyclic molecules, peptides-like, aromatic, and carbohydrates-like components than glucose-fed SMPs in the stationary and senescent phases. These components played a significant role in irreversible membrane fouling, as evidenced in UF experiments. Standard blocking and cake filtration were the main fouling mechanisms for the filtration of SMPs collected in the exponential and stationary/senescent phases, respectively. Our findings highlight linkages between SMP compositions and membrane fouling at both the compound and molecular levels and suggest that both the carbon source and growth phase strongly determine the production potential, chemical nature, and fouling behavior of SMPs.

Deciphering the succession dynamics of dominant and rare taxa is crucial to understand the stability and ecosystem functions of biofilm communities. However, the essential laws of the succession dynamics based on dominant and rare taxa were still unenlightened. Herein, we investigated the succession dynamics of dominant and rare genera in multi-species biofilms developed in flow cells fed with 10 and 40 mg-TOC/L LB broth. The relative abundance of dominant genera (Enterobacteria and Acinetobacter) decreased remarkably (from 94.63% to 73.22%) in 10 mg-TOC/L LB broth, whereas they kept relatively steady (93.75 ± 4.23%) along with the cultivation time in 40 mg-TOC/L LB broth. Fluorescence in situ hybridization showed that rare genera tended to form clusters at both concentrations, while weaker dispersal of dominant genera caused patchier biofilm structures in 10 mg-TOC/L LB broth compared to that in 40 mg-TOC/L LB broth. Null model analyses further demonstrated that the stochastic ecological drift was more pronounced in the community assembly of biofilms in 10 mg-TOC/L LB broth (73.33%) than those in 40 mg-TOC/L LB broth (60.95%), weakening the competitive superiority of dominant taxa in the patchier biofilms. In addition, the co-occurrence network reflected that the positive interactions among rare genera contributed to exclude dominant genera in 10 mg-TOC/L LB broth, whereas negative interactions only occurred between the dominant Enterobacter and Acinetobacter or rare Comamonas in 40 mg-TOC/L LB broth. This study highlighted the distinctive succession dynamics of dominant and rare genera in biofilms at different substrate concentrations, which would advance our understanding of the biofilm communities in biofilm-related process.

Domestic wastewater and agricultural runoff are increasingly viewed as major threats to both aquatic and terrestrial ecosystems due to the introduction of non-point source inorganic (e.g., nitrogen, phosphorus and metals) and organic (e.g., pesticides and pharmaceutical residues) pollutants. With rapid economic growth and social change in rural regions, it is important to examine the treatment systems in rural and remote areas for high efficiency, low running costs, and minimal maintenance in order to minimize its influence on water bodies and biodiversity. Recently, the use of vegetated drainage ditches (VDDs) has been employed in treatment of domestic sewage and agricultural runoff, but information on the performance of VDDs for treating these pollutants with various new management practices is still not sufficiently summarized. This paper aims to outline and review current knowledge related to the use of VDDs in mitigating these pollutants from domestic sewage and agricultural runoff. Literature analysis has suggested that further research should be carried out to improve ditch characteristics and management strategies inside ditches in order to ensure their effectiveness. Firstly, the reported major ditch characteristics with the most effect on pollutant removal processes (e.g., plant species, weirs, biofilms, and substrates selection) were summarized. The second focus concerns the function of ditch characteristics in VDDs for pollutant removal and identification of possible removal mechanisms involved. Thirdly, we examined factors to consider for establishing appropriate management strategies within ditches and how these could influence the whole ditch design process. The current review promotes areas where future research is needed and highlights clear and sufficient evidence regarding performance and application of this overlooked ditch system to reduce pollutants.

The effect of support material pore size on the filtration behaviors during start-up and stabilized stages in the dynamic membrane bioreactors (DMBR) was studied. Before the dynamic membrane (DM) was formed, the turbidity at 50-μm could be more than 250 NTU, while it was less than 40 and 10 NTU at 25- and 10-μm, respectively. After the DM was formed, the stabilized stage lasted for 61 days with low transmembrane pressure0.6 kPa and the 5-, 10-, and 25-μm filters had similar effluent turbidity (1 NTU) and chemical oxygen demand. However, their averaged flux was 66.4, 25.1, and 3.5 L·m

The major limitation of optimization models applied previously for rainwater harvesting (RWH) systems is the systematic evaluation of environmental and human health impacts across all the lifecycle stages. This study integrated life cycle assessment (LCA) into a multi-objective optimization model to optimize the construction areas of green rooftops, porous pavements and green lands in Beijing of China, considering the trade-offs among 24 h-interval RWH volume (QR), stormwater runoff volume control ratio (R), economic cost (EC), and environmental impacts (EI). Eleven life cycle impact indicators were assessed with a functional unit of 10,000 m2 of RWH construction areas. The LCA results showed that green lands performed the smallest lifecycle impacts of all assessment indicators, in contrast, porous pavements showed the largest impact values except Abiotic Depletion Potential (ADP) elements. Based on the standardization results, ADP fossil was chosen as the representative indicator for the calculation of EI objective in multi-objective optimization model due to its largest value in all RWH systems lifecycle. The optimization results for QR, R, EC and EI were 238.80 million m3, 78.5%, 66.68 billion RMB Yuan, and 1.05E + 16 MJ, respectively. After the construction of optimal RWH system, 14.7% of annual domestic water consumption and 78.5% of maximum daily rainfall would be supplied and controlled in Beijing, respectively, which would make a great contribution to reduce the stress of water scarcity and water logging problems. Green lands have been the first choice for RWH in Beijing according to the capacity of rainwater harvesting and less environmental and human impacts. Porous pavements played a good role in water logging alleviation (R for 67.5%), however, did not show a large construction result in this study due to the huge ADP fossil across the lifecycle. Sensitivity analysis revealed the daily maximum precipitation to be key factor for the robustness of the results for three RWH systems construction in this study.

Currently, membrane biofouling in membrane bioreactors (MBRs) is normally attributed to the occurrence of abundant bacterial species on membranes, whereas the roles of low-abundance bacteria have not been paid sufficient attention. In this study, the linear discriminant analysis (LDA) effect size (LEfSe) algorithm was used to identify active biomarkers, determining 67 different phylotypes among Bulk sludge, low-fouling Bio-cake (10 kPa), high-fouling Bio-cake (25 kPa) and Membrane pore in a membrane bioreactor with NaOCl backwash. Interestingly, a large proportion of the active biomarkers in bio-cake samples, such as Methylophilaceae, Burkholderiaceae, Paucibacter and Pseudoxanthomonas, did not fall within the abundant taxa (i.e.

Integrated fixed-film activated sludge combined with a membrane bioreactor (IFAS-MBR) is a promising alternative to improve biological nutrient removal (BNR). The synergistic benefits of the attached-growth biofilm on reactor performance have been extensively reported, yet the effects of moving carriers on driving changes in the bulk sludge bacterial community are poorly documented. In this work, two parallel MBRs (i.e., with and without carriers) were operated to determine the roles of moving carriers in shifting the bacterial community composition, particularly the active microbiota, and their impact on BNR. The results showed that the presence of moving carriers significantly improved phosphorus removal (p < 0.01) during stable operation, which was in good agreement with the higher specific phosphorus release/uptake rates of the bulk sludge from the carrier-dosed MBR compared to that from the control MBR determined by a series of batch tests. LIVE/DEAD BacLight viability staining and the use of propidium monoazide (PMA) together with 16S rRNA amplicon sequencing indicated that the bulk sludge in the carrier-dosed MBR had a higher viability and biodiversity within the active bacterial community compared with that in the control MBR, attributable to the mechanically induced decrease in floc size and an increase in mass transfer. Notably, a higher abundance of phosphate-accumulating organisms (PAOs) and lower GAOs/PAO ratio (GAOs: glycogen-accumulating organisms) were observed in the active microbiota of the carrier-dosed MBR than in the control MBR. By contrast, the abundance of ammonia oxidizing bacteria, nitrite oxidizing bacteria and denitrifiers was comparable in both MBRs. Intriguingly, these functional bacteria had highly divergent abundances between the total and active bacterial communities, likely attributable to their different susceptibilities to oxygen and substrates. Overall, this study substantially extends our understanding of the roles of fluidized particles in mediating microbiota changes in an IFAS-MBR system.

The traditional anoxic/aerobic process (A/O) process is widely used for treating digested piggery wastewater, but the lack of carbon sources leads to poor efficiency. Therefore, the process needs optimization to achieve high-efficiency and low-cost operation mode. In this study, an improved A/O system with bionic aquatic weed and Myriophyllum sp. was established to decontaminate digested piggery wastewater. The average removal efficiencies of chemical oxygen demand (COD), NH4+-N, and total nitrogen (TN) by the improved A/O system was satisfactory. The average removal efficiencies of COD, NH4+-N, and TN were 62.1%, 87.5%, and 61.9%, respectively. High-throughput sequencing identified a number of dominant microorganisms. The relative abundance of Nitrosomonas (ammonia-oxidizing bacteria) and Nitrospira (nitrite-oxidizing bacteria) was 0.07%-3.52% and 0.32%-1.30%, respectively. Combining bionic aquatic weed and Myriophyllum sp. altered the microbial community structure and metabolic pathways. The results demonstrate a cost-effective method for treating digested piggery wastewater.

In this study, we proposed a novel IFAS-MBR with low aeration for the treatment of real municipal wastewater. With biocarriers packed in the anoxic tank, the pilot-scale IFAS-MBR operated with average dissolved oxygen concentrations of 0.56 mg/L in the oxic tank. Over 110 days of operation, highly efficient nutrient removal was achieved with the total nitrogen (TN) and phosphorus (TP) removal efficiencies of 78.1 ± 7.2% and 93.7 ± 5.8%, respectively. The average effluent concentrations of TN and TP reached 5.4 and 0.26 mg/L, respectively. Meanwhile, the removal efficiency of COD reached 95.3 ± 1.3% in the system, and the concentrations of COD decreased from 31.9 ± 3.7 (sludge supernatant) to 12.7 ± 1.6 mg/L (permeate) after membrane filtration. Microbial community analysis showed that Nitrosomonas (0.32%) and Nitrospira (1.85%) in activated sludge were the main drivers of the nitrification process, while various denitrifying bacteria in activated sludge and biofilms were responsible for nitrate reduction in the anoxic tank. Candidatus Accumulibacter (0.34%) and Dechloromonas (1.31%) primarily contributed to denitrifying phosphorus uptake in the anoxic tank. Furthermore, these organisms (i.e., core functional microbiota) exhibited stable levels over the entire operation. The highly enriched hydrolytic fermentation bacteria drove community succession, and the remarkable functional robustness of microbial communities in activated sludge and biofilms favored nutrient removal. Overall, the novel IFAS-MBR system provides an energy-efficient MBR alternative owing to its highly efficient performance and low operating costs enabled by low aeration rates and the absence of an external carbon source.

While the membrane bioreactor (MBR) technology has been broadly applied to treat wastewater, a rational assessment of its techno-economy is required for its sustainable development. In this study, we performed cost-benefit analysis (CBA) and data envelopment analysis (DEA) of 35 large-scale MBRs (each with a treatment capacity of ≥10,000 m3/d). Based on the investment and operation data, we evaluated the marginal cost and environmental benefit (as a function of the shadow prices of pollutants) and hence obtained the net profit (averagely ∼35 CNY/m3 or ∼4.9 USD/m3). According to the non-radial directional distance function (NDDF) approach in the DEA framework, the average cost efficiency and energy efficiency of the MBRs were 0.77 and 0.66, respectively. The distributions of the net profit and cost/energy efficiencies significantly varied according to the geographical location, effluent standard, and operating year of the MBRs as indicated by the results of nonparametric tests. Economic panel modeling showed that the cost/energy efficiencies were also affected by external factors such as regional economic level and population density. The techno-economy of MBRs is prominent on occasions of strict effluent standards and underground locations, and could be improved by further technological advancements and policy incentives.

The composition and distribution characteristics of bacterial communities in biofilms attached to hydraulic concrete structure (HCS) surfaces were investigated for the first time in four reservoirs in the middle and lower reaches of the Yangtze River Basin using 16S rRNA Miseq sequencing. High microbial diversity was found in HCS biofilms, and notable differences were observed in different types of HCS. Proteobacteria, Cyanobacteria and Chloroflexi were the predominant phyla, with respective relative abundances of 35.3%, 25.4% and 13.0%. The three most abundant genera were Leptolyngbya, Anaerolineaceae and Polynucleobacter. The phyla Beta-proteobacteria and Firmicutes and genus Lyngbya were predominant in CGP, whereas the phyla Cyanobacteria and Chloroflexi and genera Leptolyngbya, Anaerolinea and Polynucleobacter survived better in land walls and bank slopes. Dissolved oxygen, ammonia nitrogen and temperature were characterized as the main factors driving the bacterial community composition. The most abundant groups of metabolic functions were also identified as ammonia oxidizers, sulphate reducers, and dehalogenators. Additionally, functional groups related to biocorrosion were found to account for the largest proportion (14.0% of total sequences) in gate piers, followed by those in land walls (11.5%) and bank slopes (10.2%). Concrete gate piers were at the greatest risk of biocorrosion with the most abundant negative bacterial groups, especially for sulphate reducers. Thus, it should be paid high attention to the biocorrosion prevention of concrete gate piers. Overall, this study contributed to the optimization of microbial control and the improvement of the safety management for water conservation structures.

Urban rainwater management need to achieve an optimal compromise among water resource augmentation, water loggings alleviation, economic investment and pollutants reduction. Rainwater harvesting (RWH) systems, such as green rooftops, porous pavements, and green lands, have been successfully implemented as viable approaches to alleviate water-logging disasters and water scarcity problems caused by rapid urbanization. However, there is limited guidance to determine the construction areas of RWH systems, especially for stormwater runoff control due to increasing extreme precipitation. This study firstly developed a multi-objective model to optimize the construction areas of green rooftops, porous pavements and green lands, considering the trade-offs among 24 h-interval RWH volume, stormwater runoff volume control ratio ( R ), economic cost, and rainfall runoff pollutant reduction. Pareto fronts of RWH system areas for 31 provinces of China were obtained through nondominated sorting genetic algorithm. On the national level, the control strategies for the construction rate (the ratio between the area of single RWH system and the total areas of RWH systems) of green rooftops ( η GR ), porous pavements ( η PP ) and green lands ( η GL ) were 12%, 26% and 62%, and the corresponding RWH volume and total suspended solids reduction was 14.84 billion m 3 and 228.19 kilotons, respectively. Optimal η GR , η PP and η GL in different regions varied from 1 to 33%, 6 to 54%, and 30 to 89%, respectively. Particularly, green lands were the most important RWH system in 25 provinces with η GL more than 50%, η GR mainly less than 15%, and η PP mainly between 10 and 30%. Results also indicated whether considering the objective MaxR made a non-significant difference for RWH system areas whereas exerted a great influence on the result of stormwater runoff control. Maximum daily rainfall under control increased, exceeding 200% after the construction of the optimal RWH system compared with that before construction. Optimal RWH system areas presented a general picture for urban development policy makers in China.

Effluent organic matter (EfOM) from municipal wastewater treatment plants potentially has a detrimental effect on both aquatic organisms and humans. This study evaluated the removal and transformation of chromophoric dissolved organic matter (CDOM) and fluorescent dissolved organic matter (FDOM) in a full-scale wastewater treatment plant under different seasons. The results showed that bio-treatment was found to be more efficient in removing bulk DOM (in term of dissolved organic carbon, DOC) than CDOM and FDOM, which was contrary to the disinfection process. CDOM and FDOM were selectively removed at various stages during the treatment. Typically, the low molecular weight fractions of CDOM and protein-like FDOM were more efficiently removed during bio-treatment process, whereas the humic-like FDOM exhibited comparable decreases in both bio-treatment and disinfection processes. Overall, the performance of the WWTP was weak in terms of CDOM and FDOM removal, resulting in enrichment of CDOM and FDOM in effluent. Moreover, the total removal of the bulk DOM (P

Chemical cleaning is an essential process for the permeability recovery of fouled membranes, which is highly related to the interactions between chemicals and bio-macromolecules in fouling layers. In this study, three bio-macromolecules (i.e., effluent biopolymers (i.e., 0.45 μm–100 kDa) from a full-scale municipal wastewater treatment plant, bovine serum albumin (BSA) and dextran) were exposed to different chemicals (i.e., NaClO, H2O2, NaOH, and HCl) with varied concentrations to understand the changes in their properties and functional groups. The results showed that exposure to oxidants and alkali decreased the consistency index of all bio-macromolecules. With an increased oxidant dose, the molecular sizes of effluent biopolymers and dextran continuously reduced because of the oxidative cleavage of the long molecule chains. However, the molecular size of BSA sharply increased after being treated with oxidants and alkali, likely due to the cross-linkage of protein molecules. Three-dimensional fluorescence excitation-emission matrix (3D-EEM) spectra showed that the aromatic protein-like and humic substances in the effluent biopolymers were destructed readily during the treatments of oxidants and alkali. Fourier transform infrared (FTIR) and X-ray photoelectron spectroscopy (XPS) analyse further confirmed that exposures to NaClO, H2O2 and NaOH led to the destruction of protein structures (i.e., amide I, II and III), the increase of carbonyl and carboxyl groups, and the decrease of fatty acids/lipids, all of which could make the bio-macromolecules more hydrophilic. Most importantly, the bio-macromolecules exposed to chemicals had better filterability, and their permeability through membranes also significantly increased, which could be explained well by the above analysis. The chemical cleaning mechanisms of fouled membranes are understood in depth in this study, and all of the results shed light on the implementation of on-line chemical enhanced backwashing in membrane processes.

The influence of diel temperature variations (DTVs) on nitrogen removal and bacterial communities was investigated in two parallel anammox reactors (i.e., control and DTV reactors). The control reactor was operated at a constant temperature of 30 °C, whereas the DTV reactor was operated in a temperature fluctuation mode with a cycle of 12/12 h of high/low temperatures. Nine water temperature variations for the day/night periods were set from 30/30 °C (i.e., Δ0 °C) to 38/22 °C (i.e., Δ16 °C). An increase in DTVs from Δ8 °C (34/26 °C) to Δ16 °C (38/22 °C) caused a significant decline in reactor performance and a shift in bacterial diversity. Compared to the control reactor, for instance, nitrogen removal efficiency decreased (P 0.05) when temperature fluctuations exceeded Δ8 °C in the DTV reactor with a decreasing ΔNO

International audience; Quorum sensing (QS) and quorum quenching (QQ) are increasingly reported in biological wastewater treatment processes because of their inherent roles in biofilm development, bacterial aggregation, granulation, colonization, and biotransformation of pollutants. As such, the fundamentals and ubiquitous nature of QS bacteria are critical for fully understanding the process of the wastewater treatment system. In this article, the details of QS-based strategies related to community behaviors and phenotypes in wastewater treatment systems were reviewed. The molecular aspects and coexistence of QS and QQ bacteria were also mentioned, which provide evidence that future wastewater treatment will indispensably rely on QS-based strategies. In addition, recent attempts focusing on the use of QQ for biofilm or biofouling control were also summarized. Nevertheless, there are still several challenges and knowledge gaps that warrant future targeted research on the ecological niche, abundance, and community of QS and QQ-bacteria in environmental settings or engineered systems. (C) 2019 Elsevier Ltd. All rights reserved.

Anammox-related processes are often applied for the wastewater treatment which contains both ammonium and antibiotics. Herein, the long-term effects of tetracycline (TC), at environmentally and industrially relevant concentrations, on the performance, anammox activity and microbial community of anammox reactors were investigated for 518 days. The control reactor (without TC exposure) was stable for nitrogen removal during the long-term operation (a nitrogen removal rate of 0.56 ± 0.05 kg-N·m−3·d−1). In the TC-added reactor, the nitrogen removal efficiency increased slightly at low TC levels (1–100 μg/L), whereas poor anammox performance occurred at high TC concentration (1000 μg/L). Furthermore, the concentrations of extracellular polymeric substances (EPS) were much higher at 10 μg/L than those in the control reactor (P

Ultrafiltration (UF) can achieve excellent removal of natural organic matter (NOM), but the main challenge for this process is the limited understanding of membrane fouling. The objective of this study is to explore the potential of UV-vis spectroscopic analysis for the detection of membrane fouling caused by humic acids (HA) at different solution chemistries (i.e., calcium ions (Ca(2+)) and pH). In the presence of Ca(2+), several spectral parameters, including the DSlope(325-375) (the slope of the log-transformed absorbance spectra over 325-375 nm), S(275-295) (the slope of the absorption coefficient over 257-295 nm) and S(R) (the ratio of S(275-295) to S(350-400)) of various HA solutions, were correlated with the molecule aggregation and the membrane fouling potential. Interestingly, increased DSlope(325-375) and decreased S(275-295) and S(R) were observed for the HA-Ca(2+) interaction under alkaline conditions (i.e., pH = 9) relative to those in lower pH environments (i.e., pH = 7 or 6), suggesting that spectral parameters were able to predict HA-Ca(2+) interactions under varying pH conditions. The strong correlations between the spectral parameters and the unified membrane fouling index (UMFI) obtained from UF experiments further corroborated that the spectral parameters were able to predict the membrane fouling potential. Moreover, the spectral parameters were also found to well reveal the fouling extent of the mixture of HA and Suwannee River NOM (SRNOM) or the pure SRNOM added with varying calcium concentrations, implying that the spectroscopic analysis was also available for the indication of practical NOM fouling. In addition, the measurement of S(275-295) and S(R) of the permeate solution suggests an increasing proportion of small-molecule HA in the permeate during the UF process. This study not only expands our knowledge of NOM-Ca(2+) aggregates as well as their role in membrane fouling behavior but also provides an approach for the in situ characterization of membrane performance.

Wanted and unwanted surface-attached growth of bacteria is ubiquitous in natural and engineered settings. Normally, attachment of planktonic cells to media surfaces initiates biofilm formation and fundamentally regulates biofilm assembly processes. Here, culturing biofilm with planktonic sludge as source community, we found distinct succession profiles of biofilm communities sourced from the size-fractionated sludge flocs ( 120 μm). Null model analyses revealed that deterministic process dominated in biofilm community assemblies but decreased with decreasing floc size. Additionally, the relative importance of environmental selection increased with increasing floc size of the source sludge, whereas homogenizing dispersal and ecological drift followed opposite trends. Phylogenetic molecular ecological networks (pMENs) indicated that species interactions were intensive in biofilm microbiota developed from large-sized flocs (>120 μm), as evidenced by the low modularity and harmonic geodesic distance and the high average degree. Intriguingly, the keystone taxa in these biofilm ecological networks were controlled by distinct interaction patterns but all showed strong habitat characteristics (e.g., facultative anaerobic, motile, hydrophobic and involved in extracellular polymeric substance metabolism), corroborating the crucial roles of environmental filtering in structuring biofilm community. Taken together, our findings highlight the role of planktonic floc properties in biofilm community assembly and advance our understanding of microbial ecology in biofilm-based systems.

Nitrate production during anammox can decrease total nitrogen removal efficiency, which will negatively impact its usefulness for the removal of nitrogen from waste streams. However, neither the performance characteristics nor physiological shifts associated with nitrate accumulation in anammox reactors under different nitrogen loading rates (NLRs) is well understood. Consequently, these parameters were studied in a lower NLR anammox reactor, termed R1, producing higher than expected levels of nitrate and compared with a higher NLR reactor, termed R2, showing no excess nitrate production. While both reactors showed high NH4+-N removal efficiencies (>90%), the total nitrogen removal efficiency (

Temperature is a well-known environmental stress that influences both microbial metabolism and community structure in the biological wastewater treatment systems. In this study, responses of biological performance and sludge microbiota to the long-term day/night temperature differences (DNTD) were investigated in membrane bioreactors (MBRs). The results showed that the functional bacteria could sustained their ecological functions at low DNTD (20/30 °C), resulting in relatively stable performance with respect to nutrient removal. However, when the activated sludge was subjected to a high DNTD (17/33 °C), the effluent concentrations of COD, TN and TP were significantly higher in MBR-B than that in MBR-A. In addition, more severe membrane fouling occurred under the perturbation of high DNTD as revealed by the transmembrane pressure (TMP) profile, which was mainly attributed to the accumulation of extracellular polymeric substances (EPS). The results of 16S rRNA gene sequencing showed that DNTD showed negligible effect on the bacterial community structures. Nonetheless, the functional bacteria responded differently to DNTD, which were in accordance with the bioreactor performances. Specifically, Nitrospina (NOB) and Tetrasphaera (PAOs) appeared to be sensitive to both low and high DNTD. In contrast, a low DNTD showed marginal effects on the denitrifiers, while a high DNTD significantly decreased their abundances. More strikingly, filamentous bulking bacteria were found to be well-adapted to DNTD, indicating their tolerance to the daily temperature fluctuation. This study will advance our knowledge regarding the response of microbial ecology of activated sludge to daily temperature variations in full-scale MBRs.

Anaerobic ammonium oxidation (ANAMMOX) processes can potentially be influenced by salinity related to variable salinity in water environment. Here, we used 16S rRNA sequencing analysis combining with iTRAQ-based quantitative proteomic approach to reveal the response of microbial community and functional proteins to salinity, which was increased from 0 to 20 g L−1 with a step of 5 g L−1 (designed as S5, S10, S15 and S20) compared to control reactor (without salinity stress desined as S0). The 16S rRNA sequencing analysis showed that a high salinity (20 g L−1, S20) decreased the abundance of genus Candidatus Jettenia but increased that of Candidatus Kuenenia. A total of 1609 differentially expressed proteins were acquired in the three comparison groups (S5:S0, S20:S0 and S20:S5). Of these, 39 proteins co-occurred in the three salt-exposed samples. Hydrazine dehydrogenase (HDH; Q1PW30) and nitrate reductase (Q1PZD8) were up-regulated more than 3-folds in the exposure of 20 g-NaCl/L. The functional enrichment analysis further showed that some proteins responsible for ion binding, catalysis and oxidation-reduction reaction were up-regulated, which explained the physiological resilience of ANAMMOX bacteria under salinity stress. Additionally, ANAMMOX bacteria responded to salinity by modulating the electron transport systems, indicating that the cells retained a high potential for proton pumping, as well as the ATP production. Furthermore, the over-expression of HDH which associated with ANAMMOX metabolism, was potentially related to the increased abundance of halophilic Candidatus Kuenenia. These findings provide a comprehensive baseline for understanding the roles of salinity stresses in shaping the functional proteins of ANAMMOX bacteria.

Sunlight-initiated photodegradation has a great impact on the composition and properties of natural dissolved organic matter (DOM) in aquatic environments, which potentially changes the behavior and roles of DOM in water treatment facilities. Here, we explored the effect of sunlight irradiation on membrane fouling behavior of two natural DOM (i.e., Aldrich humic acid (AHA) and Suwannee River DOM (SRNOM)), particularly in the presence of calcium ion (Ca(II)). Results showed that a long-term exposure (3 months) to sunlight during the summer led to decreases in the chromophores and molecular size of both DOM. The characterization by UV–vis spectral parameter DSlope350–400 (the slope of the log-transformed absorbance spectra in the range of 350–400 nm) indicated that sunlight-exposed DOM had a weaker Ca(II)-binding ability than unirradiated DOM, which could be attributable to the photochemically induced loss of carboxyl and phenolic groups. Additionally, AHA was found to be more susceptible to sunlight irradiation and Ca(II) addition than SRNOM, likely due to its higher aromaticity. Crucially, dead-end ultrafiltration tests showed that sunlight exposure of both AHA and SRNOM can reduce their fouling potential in the absence of Ca(II) and the presence of low Ca(II) (0.4 mM). In contrast, the addition of higher Ca(II) concentrations (2 and 3.6 mM) led to an increase in their fouling propensities. Overall, sunlight exposure can greatly alter the fouling behavior of natural DOM. This study provides a nexus between the naturally occurring transformation of DOM and its behavior (i.e., membrane fouling) in water treatment facilities.

The occurrence and fate of antibiotics in biological wastewater treatment facilities have given rise to significant concerns. This study assessed the effects of fluoroquinolones (FQs), one class of representative antibiotics in wastewater, on the performance and the bacterial community of an anoxic–aerobic membrane bioreactor operated for one year. Results show that addition of FQs to feed wastewater caused significant increases of chemical oxygen demand (COD) in the mixed liquor. Nitrogen species in permeate increased slightly after the addition of FQs but then decreased to steady levels after long-term adaptation. However, phosphorous removal was consistently poor after FQs addition. 16S rRNA gene-targeted 454-pyrosequencing revealed a sharp decrease in the microbial species richness after FQs addition and significant fluctuations of bacterial community structure at both phylum and genus levels. Members of the two dominant phyla (Proteobacteria and Bacteroidetes) appeared to be well-adapted to the FQs. Moreover, changes in relative abundance of genera (e.g., Nitrospira, Dechloromonas, Acidovorax and Opitutus) involved in nitrogen cycling were in agreement with the reactor performance of nitrification and denitrification. Clone library analysis of the amoA gene further revealed a drastic shift of dominant ammonia-oxidizing bacteria (AOB) from the Nitrosomonas communis lineage (before FQs addition) to the Nitrosomonas oligotropha lineage (after FQs addition). Batch tests on the nutrient removal and FQs biodegradation further supported the changes in microbial community structure. This study provides a much-needed look at the bacterial community dynamics in activated sludge bioreactors for the treatment of antibiotics-containing wastewater.

Numerous reports have documented the interactions of fluorescent dissolved organic matter (FDOM) with other compounds such as metals and trace contaminants by characterizing the fluorescence quenching of the FDOM components. As FDOM is composed of numerous components, inter-component interactions can potentially take place. This study investigated the interactions between protein-like and humic-like components in FDOM using titration experiments and end-member mixing tests. We found that the co-occurrence of protein-like and humic-like components in FDOM samples resulted in an overlap behavior between their fluorescence peaks related to inter-component interactions. Our results suggest that the fluorescence of the protein-like components could be greatly quenched by the humic-like components in the FDOM samples, e.g., the humic-like components from Suwannee River and Nordic Reservoir FDOM yielded significant quenching effect for tyrosine (52% and 46%, respectively) and tryptophan (35% and 36%, respectively) in the titration experiments. The fluorescence of the humic-like components, however, was not impacted by the protein-like components. With the help of complexation modeling, we found that the binding capability between protein-like and humic-like components was dependent on their sources. This study could enhance our current knowledge on the role of FDOM in water and it is also important to the monitoring of FDOM by fluorescence spectroscopy.

Wastewater-derived organic matter (WOM) is an important constituent of discharge to urban rivers and is suspected of altering the naturally occurring dissolved organic matter (DOM) in water systems. This study investigated sunlight-induced changes in chromophores and fluorophores of WOM with different salinities (S = 0, 10, 20 and 30) that were collected from two wastewater treatment plants (WWTP-A and WWTP-B). The results showed that exposure to sunlight for 5.3 × 10(5) J/m(2) caused significant decreases in UV254-absorbing WOM (45-59% loss) compared to gross dissolved organic carbon (

Partial nitritation is an indispensable pretreatment for anaerobic ammonium oxidation process. However, the nitritation is limited by the low growth rates of ammonia-oxidizing bacteria (AOB). In this study, a membrane bioreactor (MBR) was operated for 300 days to assess its nitritation performance and the shift of microbial community. Results showed that the reactor obtained satisfying nitritation after a startup period of 50 days, which finally achieved ammonium conversation rates of about 0.8 kg N/m3/d. The apparent half-saturation constant (Km) and maximum ammonium oxidation rate (rmax) of the AOB-enriched culture were determined to be 6.1 mg N/L and 1.1 kg N/g-VSS/d, respectively. In addition, lower fouling rates were found in the initial operating days that the reactor was fed with lower ammonium loads (day 0–day 150). However, the increased ammonium loads (>0.6 kg N/m3/d) in the following 150 days resulted in increases in extracellular polysaccharides, leading to much higher fouling rates. 16S rRNA high-throughput sequencing analysis showed clear changes in the microbial community populations during the MBR operation. Results also showed that ordinary heterotrophic organisms and nitrite-oxidizing bacteria were successively inhibited; finally Nitrosomonas dominated in the nitritation MBR, with relative abundance of 40–46%. Moreover, the AOB-enriched culture was of higher microbial diversity than the seeding sludge. This study could not only improve our understanding of the bacterial community dynamics in nitritation processes, but also provide more alternatives for MBR applications.

Extracellular polymeric substances (EPSs) are key biological substances, which largely determine properties of sludge flocs, including hydrophobicity, adhesion, flocculation, settling and dewatering properties, and therefore significantly affect membrane fouling in membrane bioreactors (MBRs). Much progress has been achieved in understanding of EPSs and their interrelations with membrane fouling due to a large number of systematic papers published in the last three decades. In this paper, the fundamentals of EPSs including definitions, compositions and properties are summarized. The interrelations of EPSs with other foulants in MBRs are clarified. Roles of EPSs in membrane fouling mechanisms in MBRs are critically assessed. Furthermore, factors affecting EPSs production and characteristics are summarized, and based on which, EPSs control towards membrane fouling mitigation in MBRs are comprehensively discussed. Finally, future research perspectives regarding EPSs and membrane fouling are proposed.

Based on conventional chemical cleaning and physical backflush methods, a novel in situ chemical backflush method, i.e., chemically assisted maintenance cleaning with NaClO as the principal reagent, was developed for membrane fouling control in membrane bioreactors (MBRs). The results demonstrated that, compared with a control MBR with water backflush, the use of low NaClO loads had few adverse effects on nutrient removal; on the contrary, the exposure to NaClO enhanced the denitrification performance of the MBR as a result of the formation of sludge granules. Measurements of transmembrane pressure (TMP) showed that an NaClO backflush at 0.2 ppm could achieve effective membrane fouling control in MBRs. Ex situ backflush tests showed that an NaClO backflush enhanced the detachment of biopolymers from the fouled membranes compared with a water backflush. Comparative 16S rRNA sequencing showed differing bacterial community composition in the fouling layers of the two MBRs. Specifically, the NaClO backflush could suppress filament-caused membrane fouling (i.e., lowered the abundance of Thiothrix eikelboomii in the fouling layers). Both the water and NaClO backflush resulted in significant increases in the pure water permeability of the membranes as a result of the enlargement of membrane pores. The results of Fourier transform infrared spectrometry indicated that the frequent NaClO backflush did not change the functional groups of the active layer of the membranes significantly. This study could provide an alternative for the implementation of membrane cleaning in MBR plants.

Anthropogenic activities have dramatically changed the loads and compositions of dissolved organic matter (DOM) in urbanized streams. In this study, the spatial and temporal variations of DOM in the anthropogenically impacted Zhujiang River were investigated by analyzing the water samples in an upstream, urbanized area and downstream of the rivers on different days of one year. The results indicated that the levels of dissolved organic carbon (DOC) and total phosphorus (TP) were unaffected by seasonal changes, but the specific UV254 absorbance (SUVA) values and the total nitrogen (TN) content were greater in the winter than those in the summer. Parallel factor (PARAFAC) analysis of the excitation emission matrices (EEM) revealed the presence of three anthropogenically derived components [tryptophan-like (C1) and tyrosine-like proteins (C3) and anthropogenic humic substances (C5)] in the urbanized rivers, and they had greater seasonal and spatial variability than the terrestrial and microbial humic substances (C2 and C4). Cluster analysis revealed that treated wastewater was an important source of DOM in the urbanized streams. Photodegradation experiments indicated that the DOM in the populous area of the rivers had greater photodegradation potentials than that in the downstream region or in the natural waters. Interestingly, that the anthropogenic humic substances (C5) were considerably more photoreactive than the other four PARAFAC components, which exhibited a decrease of 80% after exposure to sunlight for 0.5 d. This study suggests that the treated wastewater could be an important input to the DOM in the urbanized rivers and the naturally occurring photodegradation could help in eliminating the anthropogenic DOM during their transport.