1. Characterization of Staphylococcus aureus strains associated with food poisoning outbreaks in France
- Author
-
Jacques-Antoine Hennekinne, Annaëlle Kerouanton, L. Petit, C. Letertre, Anne Brisabois, O. Chesneau, M.L. De Buyser, Agence Française de Sécurité Sanitaire des Aliments (AFSSA), Agents antibactériens, Institut Pasteur [Paris], We acknowledge the technical assistance of Trinh Tam Dao, Marie-Odile Delbart, Muriel Marault, and Anne Morvan. We dedicate this paper to the memory of Névine El Solh who contributed to the phage-typing and who passed away in January 2005., and Institut Pasteur [Paris] (IP)
- Subjects
Salmonella ,Enterotoxin ,MESH: Base Sequence ,medicine.disease_cause ,SmaI ,Disease Outbreaks ,MESH: Genotype ,Enterotoxins ,Genotype ,MESH: Staphylococcus aureus ,MESH: Enterotoxins ,Cluster Analysis ,MESH: Disease Outbreaks ,MESH: Phylogeny ,Phylogeny ,2. Zero hunger ,0303 health sciences ,General Medicine ,PFGE ,3. Good health ,Electrophoresis, Gel, Pulsed-Field ,Phenotype ,MESH: Electrophoresis, Gel, Pulsed-Field ,Staphylococcus aureus ,MESH: Staphylococcal Food Poisoning ,France ,Staphylococcal Food Poisoning ,Food Contamination ,Biology ,MESH: Phenotype ,Microbiology ,Food-poisoning ,03 medical and health sciences ,medicine ,Pulsed-field gel electrophoresis ,Humans ,MESH: Food Microbiology ,030304 developmental biology ,MESH: Humans ,Base Sequence ,030306 microbiology ,Outbreak ,MESH: Food Contamination ,MESH: Cluster Analysis ,[SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology ,MESH: France ,Food Microbiology ,Food Science - Abstract
International audience; Enterotoxins produced by Staphylococcus aureus are responsible for staphylococcal food-poisoning outbreaks (SFPO). In France, SFPO are the second cause of food-borne diseases after Salmonella. However, very little is known about the strains involved. The objective of this study was to characterize the staphylococcal strains related to these SFPO through phenotypic and genotypic analyses. A total of 178 coagulase-positive staphylococcal isolates recovered from 31 SFPO (1981-2002) were screened through biotyping. Thirty-three strains representative of the different biotypes in each SFPO were further examined for SmaI macrorestriction-type, phage-type, resistance to various antimicrobial drugs, presence of staphylococcal enterotoxin (se) genes sea to sei, and production of enterotoxins SEA to SED. All these 33 strains were identified as S. aureus species: 27 were of human biotypes and six ovine or non-host-specific biotypes. Most (74.1%) strains reacted with group III phages. Eleven strains were resistant to at least two classes of antibiotics and among them, two were resistant to methicillin. Twenty-nine strains carried one or several of the eight se genes tested; the gene sea was most common (n=23), and often linked to sed (n=12) or seh (n=5). The novel se genes seg-i were in all cases associated with se genes sea to sed except for one strain which carried only seg and sei. Pulsed-Field Gel Electrophoresis (PFGE) of SmaI macrorestriction digests of the 33 strains discriminated 32 PFGE patterns grouped into nine biotype-specific clusters. All five strains carrying sea and seh were grouped together into the same sub-cluster. Three of the four se-gene-negative strains were in one PFGE cluster: all four should be tested for se genes not included in this study and, if negative, be further investigated for the presence of unidentified SEs.
- Published
- 2007