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Pine wood nematode disease is currently the most deadly forest disease in China, and the Monochamus alternatus is its primary vector. Controlling the M. alternatus is crucial for managing pine wood nematode disease. This study, based on the selected HasA (pGHKW4) secretory expression vector, used electroporation to combine the genetically modified high-toxicity toxin Cry3Aa-T with the entomopathogenic bacterium Yersinia entomophaga isolated from the gut of the M. alternatus. The SDS-PAGE and Western blotting techniques were employed to confirm the toxin protein's secretion capability. The engineered bacteria's genetic stability and effectiveness in controlling M. alternatus were assessed for their insecticidal activity. The results of the SDSPAGE and Western blotting analyses indicate that the HasA system effectively expresses toxin protein secretion, demonstrates certain genetic stability, and exhibits high insecticidal activity against M. alternatus. This study constructed a highly toxic entomopathogenic engineered bacterial strain against M. alternatus larvae, which holds significant implications for controlling M. alternatus, laying the foundation for subsequent research and application of this strain. [ABSTRACT FROM AUTHOR]

With the advent of the era of big data and information technology, deep learning (DL) has become a hot trend in the research field of artificial intelligence (AI). The use of deep learning methods for parameter inversion, disease identification, detection, surrounding rock classification, disaster prediction, and other tunnel engineering problems has also become a new trend in recent years, both domestically and internationally. This paper briefly introduces the development process of deep learning. By reviewing a number of published papers on the application of deep learning in tunnel engineering over the past 20 years, this paper discusses the intelligent application of deep learning algorithms in tunnel engineering, including collapse risk assessment, water inrush prediction, crack identification, structural stability evaluation, and seepage erosion in mountain tunnels, urban subway tunnels, and subsea tunnels. Finally, it explores the future challenges and development prospects of deep learning in tunnel engineering. [ABSTRACT FROM AUTHOR]

Space grid structures are widely used in the field of structural engineering due to their good mechanical properties and simple construction procedures. This paper reviews the development of space grid structures and mainly introduces the development of space grid structures in China in recent years. Some typical applications of space grid structures are introduced and the technical developments are summarized. It shows that China not only has a large number of engineering applications of space grid structures, but also a rapid development of related technologies. Finally, some prospects for space grid structures are discussed. [ABSTRACT FROM AUTHOR]

This paper introduces the development process of building cable structures in China over the past 70 years according to three stages: the initial, slow, and rapid development. It also summarizes the types of cable components and joints of building cable structures. The structural characteristics and representative projects of suspension, cable membrane, beam string (cable-supported), and cable dome structures are reviewed in this paper. Finally, the achievements and prospects of cable structures in China are discussed. [ABSTRACT FROM AUTHOR]

Purpose: The purpose of this study is to systematically identify, assess, and categorize the barriers to digital transformation (DT) in the engineering and construction sectors, and thus to better understand the impact and how these sectors might be overcome. Design/methodology/approach: This study adopted a sequential mixed qualitative and quantitative data collection and analysis approach. DT barriers were first identified from relevant literature and verified by an expert panel. Then, a questionnaire survey assessing the impacts of the identified DT barriers was distributed to construction professionals in China, and 192 valid responses were retrieved. Further, the data obtained were analyzed using ranking analysis, exploratory factor analysis (EFA), and partial least squares-structural equation modeling (PLS-SEM). Findings: Based on the ranking analysis, the top three barriers are "lack of industry-specific standards and laws," "lack of clear vision, strategy and direction for DT," and "lack of support from top management for DT." EFA enabled the grouping of the 26 barriers into 3 categories: (1) lack of laws and regulations (LLR), (2) lack of support and leadership (LSL), and (3) lack of resources and professionals (LRP). The PLS-SEM analysis revealed that LLR, LSL, and LRP were found to have significant negative impacts on DT. Originality/value: These findings contribute to the body of knowledge on DT in the construction industry and help construction firms and government bodies improve the understanding of these barriers to DT and put forward relevant policies and incentives, thus seizing the DT benefits as a way to enhance construction project management. [ABSTRACT FROM AUTHOR]

Oil and gas reservoirs in volcanic rocks are a particular type of unconventional reservoir and present unique challenges for exploration and production engineers. To help the oil industry understand volcanic reservoirs and solutions to complex development problems, we reviewed their key engineering technologies as well as their geological characteristics. The distinctive geological characteristics of volcanic hydrocarbon reservoirs are strong heterogeneity, low porosity and permeability, complex fracture systems, etc. The volcanic reservoir rock types in order of hydrocarbon abundance are basalt (38.5%), andesite (15.9%), volcaniclastic (12.1%), and rhyolite (11.5%). The porosity ranges from 0.1 to 70%, and permeability ranges from 0.0007 to 762 md. In some commercially developed volcanic reservoirs of China, the average porosity is 7.7–13%; the average permeability is 0.41–3.4 md. Engineers have applied a variety of adapted technologies to produce volcanic reservoir economically. Horizontal wells can increase production and reserves by 4–6 times those of vertical wells, and longer wells are preferred. Specialized hydraulic fracturing techniques are suggested, including small or mixed proppant size, second HF treatment after proppant slugging, high-viscosity frac fluid with high-temperature resistance, special fluid loss reducer, high pump pressure, Extreme Overbalance Perforating, limited-entry fracturing, matrix acidizing, etc. Water control measures include producing below critical rates, partial perforation or penetration, controlling hydraulic fracture height, using horizontal wells, implementing complete cementing job, etc. Well productivity evaluation should be conducted to understand well performance and appropriately allocate production rates among wells, using the modified AOF method and other productivity prediction models considering breakdown fracture gradient, gas slippage effect, non-Darcy effect, etc. Well sites need to be selected based on recognizing profitable lithologies, lithofacies, high porosity and permeability, relatively developed fracture systems, thick net pay zones, etc. The critical questions for the industry are how to enhance volcanic reservoir recovery with more efficient and economic hydraulic fracturing and water control techniques. This is one of the first papers systematically summarizing the engineering technologies and unique solutions to develop volcanic reservoirs. Further and more complete reviews can be carried out in the future, and more novel and effective techniques can be explored and tested in the field. [ABSTRACT FROM AUTHOR]

The development of Xiongan New Area in Hebei Province, China, as a significant national choice, has considerable strategic significance for the integrated growth of Beijing, Tianjin, and Hebei. This paper proposes a cloud model for the suitability evaluation of the construction of Xiongan New Area based on entropy weight analysis, taking into account the geological conditions, groundwater environment, environmental geological problems, and other factors of the suitability of image city development. According to the research, the suitability evaluation findings for the project building employing the cloud model are in strong accord with those of the traditional model and have some application potential. The evaluation's findings indicate that the project construction in Xiongan New Area is acceptable, with suitable and relatively suitable sites making up 81.4% of the total area and excellent circumstances for project development, construction, and usage. This study offers helpful direction for Xiongan New Area's urban land-space design and serves as a useful point of comparison for studies looking at the viability of other deep Quaternary Plain region engineering buildings. [ABSTRACT FROM AUTHOR]

Purpose: This paper aims to establish a model to evaluate the benefits of building information modeling (BIM) from the owners' perspective. The model analyzes the correlation between five secondary benefit indicators and their subsystems: product, financial, organizational, management and strategic. The final key factors of BIM benefits provide a decision-making basis for owners to raise the efficiency of BIM application. Design/methodology/approach: Firstly, the authors combed 31 BIM-related literature and interviewed experts to identify 15 preliminary benefit indicators. The authors established a BIM benefit evaluation system based on relevant concepts, including two primary indicators, five secondary indicators and 15 tertiary indicators. Secondly, the authors analyzed the indicators by the extension theory of matter element analysis and a questionnaire survey of expert opinion. Finally, the new method was applied to a case study of a large shopping center in east China for empirical verification. Findings: A BIM benefit evaluation model, including a three-tiered hierarchy of primary, secondary and tertiary indicators, was constructed through literature review and expert opinions. The model determined the critical factors of BIM benefits, enhanced understanding of owner benefits and improved BIM application under the owners' leadership. Originality/value: At present, most studies focus on specific project stages or benefit indicators. This study developed an integrated BIM benefit evaluation system that targets owners. The findings could foster the development of China's construction industry, promote owner-led BIM application and advocate adopting the benefit evaluation method. [ABSTRACT FROM AUTHOR]

The technology and service modes of logistics industry are changing greatly in smart economy, and innovation is becoming new development engine, which promotes the urgent transformation requirement of teaching system of logistics engineering majors in universities. Based on the data from logistics engineering majors in Chinese universities in 2021, a relevant study on the undergraduate logistics engineering major is carried out. Along with 'ability-motivation-opportunity' (AMO) theory, this paper analyses the influence of ability, motivation and opportunity on the transformation. The current situation of teaching system, the existing problems and the improvement measures are investigated. Based on the above analysis, this paper puts forward the relevant suggestions to promote the cultivation of innovative talents, mainly including planning and designing the training programme system for logistics engineering major, placing more emphasis on logistics practice courses, promoting undergraduate students' participation in various logistics contests, and increasing the level of teaching investment and guarantee. [ABSTRACT FROM AUTHOR]

In order to promote the development and application process of suspended permanent maglev monorail transportation system, China has built suspended permanent maglev monorail transportation system engineering test line, Xingguo engineering test line, and the line has made useful explorations on many key technologies in the engineering process of the system. The key technical contents such as the overall layout of the test line, the suspension guidance and drive system, track beam and turnout, manufacturing process, which are different compared to the permanent maglev monorail transit and the conventional rubber wheel suspended monorail transit, are studied emphatically in this paper. The research shows that, under the premise of meeting the basic requirements of the test line, the reasonable overall layout reduces the land and engineering investment at the greatest extent. The innovative design of core systems, such as permanent maglev, realizes the stable suspension guidance and drive of the test line vehicles, and the design of key systems such as track beam bridge, turnout and processes lays the foundation for the safe operation of the test line. [ABSTRACT FROM AUTHOR]

CO2 geological storage projects are an essential tool for China to achieve the double carbon target of energy savings and emission reductions. In order to safely and effectively control the implementation of injection projects and monitor the dynamics of CO2 injection, multi-dimensional and multi-disciplinary monitoring tools are required. Among them, microseismic monitoring is a key technology for predicting reservoir dynamics and reflecting reservoir geomechanical behavior. Such monitoring has been carried out previously for reservoirs in other countries, but experimental projects are also gradually being developed in China. In this paper, we focus on the research and analysis results of microseismic monitoring of carbon storage projects in various work areas. For different reservoir conditions, we explore combinations of the monitoring implementation methods in China, comparing the differences in each work area. We propose a joint well and ground microseismic monitoring method and a multi-spatial and multi-physical field coupling research system for use in the implementation of domestic demo projects for the future research and development of microseismic monitoring of carbon storage projects. The monitoring program can meet the requirements for certain periodic repeated or continuous observations and can intelligently assess the risk and handle the alert behavior. The foundation is laid for the development of the future microseismic monitoring technology to achieve the goal of developing cost-controllable, permanent, and real-time monitoring equipment. The application of the monitoring system in China has been effective, and this experience can contribute to the development of injection engineering in the future. [ABSTRACT FROM AUTHOR]