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This work investigated engine performance and emissions using waste tire oil-diesel-biodiesel blends. A sustainable fuel glycine max biodiesel was blended with the tire oil-diesel blends to improve performance, combustion, and exhaust emissions. The seven fuels including a 100% diesel 10–30% waste tire oil to 90-70% diesel, 10% tire oil +10% biodiesel +80% diesel, 30% tire oil+10% biodiesel+60% diesel and 10% biodiesel+90% diesel was used as fuels in a direct injection diesel engine. Up to 30% (vol) waste tire pyrolysis oil was blended with diesel. More than 30% of waste tire pyrolysis oil shows inferior solubility issues and inferior engine performance and emissions. Thus, this investigation was limited to 30% waste tire pyrolysis oil. All fuel blends showed similar properties to diesel. With similar engine performance, like torque, power, efficiency, energy, and exergy metrics, the blends showed insignificant variations in emissions (carbon dioxide, nitrogen oxide) compared to a reference diesel fuel. Interestingly, the experimental results were compared with the modelling results, and the maximum variations between them were 10%. The outcome of this research can promote waste tire pyrolysis oil as an alternative fuel for diesel engines and accords with alternative energy development initiatives all over the world.