1. Promotion of vesicular stomatitis virus fusion by the endosome-specific phospholipid bis(monoacylglycero)phosphate (BMP).
- Author
-
Roth SL and Whittaker GR
- Subjects
- Animals, Cell Line, Cholesterol chemistry, Endosomes chemistry, Endosomes virology, Hydrogen-Ion Concentration, Lipids chemistry, Liposomes chemistry, Liposomes metabolism, Lysophospholipids chemistry, Membrane Glycoproteins metabolism, Monoglycerides chemistry, Phosphatidylcholines chemistry, Phosphatidylserines chemistry, Viral Envelope Proteins metabolism, Virus Internalization, Endosomes metabolism, Lysophospholipids metabolism, Membrane Fusion, Monoglycerides metabolism, Vesicular stomatitis Indiana virus metabolism
- Abstract
Vesicular stomatitis virus (VSV) is a prototypic virus commonly used in studies of endocytosis and membrane trafficking. One proposed mechanism for VSV entry involves initial fusion with internal vesicles of multivesicular endosomes followed by back-fusion of these vesicles into the cytoplasm. One feature of endosomal internal vesicles is that they contain the lipid bis(monoacylglycero)phosphate (BMP). Here, we show that the presence of BMP significantly increases the rate of VSV G-mediated membrane fusion. The increased fusion was selective for VSV and was not evident for another enveloped virus, influenza virus. Our data provide a biological rationale for a two-step infection reaction during VSV entry, and suggest that BMP preferentially affects the ability of VSV G to mediate lipid mixing during membrane fusion., (Copyright © 2011 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.)
- Published
- 2011
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