1. Use of new endophytic fungi as pretreatment to enhance enzymatic saccharification of Eucalyptus globulus.
- Author
-
Martín-Sampedro R, Fillat Ú, Ibarra D, and Eugenio ME
- Subjects
- Biotechnology methods, Hydrolysis, Laccase metabolism, Spain, Trametes physiology, Wood metabolism, Ascomycota physiology, Carbohydrate Metabolism, Endophytes physiology, Eucalyptus metabolism, Eucalyptus microbiology, Wood microbiology
- Abstract
New endophytic fungi are assessed for the first time as pretreatment to enhance saccharification of Eucalyptus globulus wood. The fungi are all laccase-producing ascomycetes and were isolated from eucalyptus trees in Spain. After five endophytes had been assayed alone or in combination with white-rot fungus Trametes sp. I-62, three were pre-selected. To improve sugar production, an autohydrolysis pretreatment was performed before or after fungal treatment. Pretreatment increased sugar production 2.7 times compared to non-pretreated wood. When fungal and autohydrolysis pretreatments were combined, a synergistic increase in saccharification was observed in all cases. Endophytic fungi Ulocladium sp. and Hormonema sp. produced greater enhancements in saccharification than Trametes sp. I-62 (increase in sugar yields of 8.5, 8.0 and 6.0 times, respectively), demonstrating the high potential of these new endophytic fungi for saccharification enhancement., (Copyright © 2015 Elsevier Ltd. All rights reserved.)
- Published
- 2015
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