1. BPDE exposure promotes trophoblast cell pyroptosis and induces miscarriage by up-regulating lnc-HZ14/ZBP1/NLRP3 axis.
- Author
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Wang, Rong, Xu, Xiaole, Yang, Jingjing, Chen, Weina, Zhao, Jingsong, Wang, Manli, Zhang, Ying, Yang, Yang, Huang, Wenxin, and Zhang, Huidong
- Subjects
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PYROPTOSIS , *TROPHOBLAST , *MISCARRIAGE , *PERSISTENT pollutants , *ENDOCRINE disruptors , *RECURRENT miscarriage - Abstract
Environmental Benzo(a)pyrene (BaP) and its ultimate metabolite BPDE (benzo(a)pyrene-7,8-dihydrodiol-9,10-epoxide) are typical persistent organic pollutants and endocrine disrupting chemicals. BaP/BPDE exposure might cause human trophoblast cell dysfunctions and induce miscarriage. However, the underlying mechanisms remain largely elusive. In this study, we found that BPDE exposure induced human trophoblast cell pyroptosis by up-regulating NLRP3/Caspase1/GSDMD pathway. We also identified that lnc-HZ14 was highly expressed in BPDE-exposed trophoblast cells and in recurrent miscarriage (RM) vs healthy control (HC) villous tissues. Lnc-HZ14 promoted trophoblast cell pyroptosis by promoting IRF1-mediated ZBP1 transcription, increasing METTL3-mediated m6A methylation on NLRP3 mRNA and its stability, and also enhancing ZBP1/NLRP3 protein interactions. Knockdown of lnc-HZ14/ZBP1/NLRP3 axis could efficiently alleviate BPDE-induced trophoblast cell pyroptosis. Higher level of pyroptosis, as indicated by the up-regulation of lnc-HZ14/ZBP1/NLRP3 axis, was found in RM vs HC villous tissues. In BaP-exposed mouse model, BaP exposure induced placental tissue pyroptosis and miscarriage by up-regulating murine Zbp1/Nlrp3 axis, and knockdown of Nlrp3 could efficiently reduce placenta pyroptosis and alleviate BaP-induced mouse miscarriage. Serum IL-1β protein level might act as a promising indicator to predict the risk of miscarriage. These findings provided new insights into BaP/BPDE-induced trophoblast cell pyroptosis and miscarriage and might be helpful for further assessment of the toxicological effects of BaP/BPDE on the female reproduction. [Display omitted] • BPDE induced trophoblast pyroptosis by up-regulating lnc-HZ14/ZBP1/NLRP3 axis. • Lnc-HZ14 promoted ZBP1 transcription, increased NLRP3 mRNA stability, and enhanced ZBP1/NLRP3 interactions. • Higher level of pyroptosis was found in RM vs HC villous tissues. • BaP induced murine placenta pyroptosis and mouse miscarriage via Zbp1/Nlrp3 axis. • IL-1β protein level in serum might predict the risk of miscarriage [ABSTRACT FROM AUTHOR]
- Published
- 2023
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