1. Viral-induced encephalitis initiates distinct and functional CD103+ CD11b+ brain dendritic cell populations within the olfactory bulb.
- Author
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D'Agostino PM, Kwak C, Vecchiarelli HA, Toth JG, Miller JM, Masheeb Z, McEwen BS, and Bulloch K
- Subjects
- Animals, Antigen Presentation immunology, Antigens, CD immunology, Antigens, CD metabolism, Antigens, Ly immunology, Antigens, Ly metabolism, Brain metabolism, Brain virology, CD11b Antigen immunology, CD11b Antigen metabolism, Cells, Cultured, Dendritic Cells metabolism, Encephalitis, Viral genetics, Encephalitis, Viral metabolism, Flow Cytometry, Integrin alpha Chains immunology, Integrin alpha Chains metabolism, Leukocyte Common Antigens immunology, Leukocyte Common Antigens metabolism, Luminescent Proteins genetics, Luminescent Proteins metabolism, Mice, Mice, Inbred C57BL, Mice, Transgenic, Microscopy, Confocal, Olfactory Bulb metabolism, Ovalbumin immunology, Receptor, Macrophage Colony-Stimulating Factor immunology, Receptor, Macrophage Colony-Stimulating Factor metabolism, Receptors, Chemokine immunology, Receptors, Chemokine metabolism, Rhabdoviridae Infections genetics, Rhabdoviridae Infections immunology, Rhabdoviridae Infections metabolism, T-Lymphocytes immunology, T-Lymphocytes metabolism, Vesicular stomatitis Indiana virus immunology, Brain immunology, Dendritic Cells immunology, Encephalitis, Viral immunology, Olfactory Bulb immunology
- Abstract
Dendritic cells (DC) are antigen-presenting cells found in both lymphoid and nonlymphoid organs, including the brain (bDC) of Cd11c/eyfp transgenic C57BL/6 mice. Using an intranasal vesicular stomatitis virus infection, we demonstrated that EYFP(+) cells amass in areas associated with viral antigens, take on an activated morphology, and project their processes into infected neuronal tissue within the olfactory bulb. These bDC separated into three EYFP(+) CD45(+) CD11b(+) populations, all but one being able to functionally promote both T lymphocyte proliferation and T(H)1 cytokine production. One population was shown to emanate from the brain and a second population was peripherally derived. The third population was of indeterminate origin, being both radiosensitive and not replenished by donor bone marrow. Finally, each EYFP(+) population contained CD11b(+) CD103(+) subpopulations and could be distinguished in terms of CD115, Gr-1, and Ly-6C expression, highlighting mucosal and monocyte-derived DC lineages.
- Published
- 2012
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