1. Construction of chiral nanozymes with high enantioselectivity for visual detection via smartphone-based paper sensors.
- Author
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Li, Qing, Wu, Congnan, Zhang, Jiaxing, Su, Rongxin, Wang, Yuefei, and Qi, Wei
- Subjects
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ENANTIOSELECTIVE catalysis , *MOLECULAR dynamics , *HYDROGEN bonding interactions , *SYNTHETIC enzymes , *CATALYTIC activity , *ENANTIOMERS - Abstract
This work constructed chiral nanozymes (Cys@Au/Fe Hops) with high enantioselectivity activity by cysteine-induced synthesis. The nanozymes, proficient in detecting DOPA enantiomers, have been transformed into a test paper sensor, offering enantioselective catalysis and enabling visual chiral detection via a smartphone platform. [Display omitted] • The Chiral Cys@Au/Fe Hops was synthesized using a one-pot method with cysteine as a chiral inducer. • Remarkable enantioselective catalytic activity for DOPA enantiomers by L/D -Cys@Au/Fe Hops. • Steric effects on DOPA enantiomers catalyzed by L/D -Cys@Au/Fe Hops revealed through molecular dynamics simulations. • Development of chiral nanozymes into stable test paper sensor for visual DOPA detection. The development of enantioselective nanozymes holds profound significance in synthesizing chiral compounds and elevating analytical precision. Herein, we have synthesized chiral gold/iron hierarchically organized particles (Cys@Au/Fe Hops) possessing peroxidase-like catalytic activity, through a one-pot method using cysteine as a chiral inducer. The nanozymes exhibited remarkable enantioselective catalytic activity to 3,4-dihydroxy-phenylalanine (DOPA) enantiomer. Notably, L -Cys@Au/Fe Hops, comprising right-handed helical nanopetals, exhibited elevated catalytic efficiency for L -DOPA over D -DOPA, whereas the reverse held for D -Cys@Au/Fe Hops. The molecular dynamics simulations demonstrated hydrogen bonding interactions between the active site and substrate, thereby revealing how steric effects affected the enantioselective catalysis of chiral DOPA by L/D -Cys@Au/Fe Hops. Moreover, the chiral nanozymes were developed into a test paper sensor due to their exceptional stability, which can achieve the visual detection of DOPA enantiomer combined with a smartphone platform. This work is significant in advancing the design and practical utility of highly selective enzyme mimics. [ABSTRACT FROM AUTHOR]
- Published
- 2024
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