Search

Despite the potential benefits of using cellulose ethers and their combination with other polysaccharides and proteins in the creation of reduced-fat water-in-oil (W/O) emulsions, research in this specific area is currently limited. Further studies are needed to explore the optimal combinations and concentrations of these materials to achieve emulsions with desired texture and higher rheological properties. To address this research gap, seven W/O emulsions with a 40 wt% water phase at pH 3.5 are prepared. The water phase consisted of 2 wt% methylcellulose (MC) or hydroxypropyl methylcellulose (HPMC) combined with 10 wt% whey protein isolate (WPI) and/or kappa-carrageenan (kC). The oil phase, comprising 60 wt%, contained 8 wt% polyglycerol polyricinoleate (PGPR) in soybean oil. The emulsions were initially prepared at room temperature and subsequently subjected to a heating process at an elevated temperature of 90 °C for a duration of 30 min. Following this heating step, the emulsions were then cooled back to room temperature. This sequential temperature treatment was employed with the intention of inducing aggregation and gelation within the emulsions. The temperature oscillation measurements (G* versus temperature) provide further evidence for the role of covalent and noncovalent bonding in the rheology and gelation of WPI-based emulsions. The viscosity and moduli of unheated and heated emulsions containing combinations of HPMC were found to be higher compared to those containing MC combinations. This behavior can be attributed to the stronger intermolecular interaction between HPMC, WPI, and/or kC, as well as their attachment with PGPR. These interactions lead to the formation of a more viscous and firmer W–O interface in emulsions. [Display omitted] • W/O emulsions prepared using cellulose ethers, WPI, and/or kappa carrageenan aggregation in the aqueous phase. • They exhibit high viscosity and elasticity and form compact microstructures when undergoing heat treatment. • HPMC combinations showed higher rheological properties compared to the equivalent MC emulsions. [ABSTRACT FROM AUTHOR]

In order to get long time stability in Water-in- Soybean oil emulsion the impact of selfstructuring lipid, polyglycerol polyricinoleate (PGPR) has been investigated. The rheological and microstructural techniques were employed to characterize the stability and flow behavior of the material. Emulsions were prepared from 10- 40 wt.% water having 2-8 wt. % Polyglycerol polyricinoleate in phosphate buffer (pH 7, NaCl 100mM). It has been observed that an increase in water contents led to decrease in stability of emulsion while the impact of Polyglycerol polyricinoleate was other way round. The shear viscosity of the system was increased with the increase in water as well as polyglycerol polyricinoleate (PGPR) contents. The stated observations were also in accord with the optical micrographs and were helpful to explain the stability as well as the rheological results. [ABSTRACT FROM AUTHOR]

The study aims to investigate the stability of W/O emulsions with respect to coalescence time. The various concentrations of water were dispersed in oil phase (soybean oil). The compositions of organic and aqueous phases were varied by adding emulsifier (Monoglyceride), sodium chloride and thickening agent (mango's pulp). The technique employed for the mixing of two phases was homogenization. The Emulsion Stability Index (ESI), Viscosity changes, separation of organic and aqueous phases as a function of storage time have been studied. It has been found that monoglyceride increases the stability and decreases the emulsion stability index (ESI) and also decreases the viscosity changes with storage time while electrolytes and mango's pulp encourage the coalescence process and enhance the instability of the system. On the other hand the system that contained all the organic and aqueous ingredients showed high stability. [ABSTRACT FROM AUTHOR]

Abstract: The objective of this work was to prepare novel microstructures and textures by controlled osmotic swelling and gelation of multiple (W1/O/W2) emulsions. Initially, multiple emulsions were prepared: internal aqueous phase (W1) containing 20wt.% whey protein isolate (WPI) in buffer solution (pH7, 250mM NaCl); oil phase (O) containing 6wt.% polyglycerol polyricinoleate (PGPR) in soybean oil; and external aqueous phase (W2) containing 250mM NaCl and 1wt.% Tween 80. These W1/O/W2 emulsions were then subjected to different degrees of dilution (1:0 to 1:5 with salt-free buffer) and thermal treatment (30 to 90°C) to induce swelling and/or gelation of the internal aqueous phase. W1/O/W2 emulsions had appreciably higher viscosities than O/W emulsions with similar fat contents, which was attributed to the increase in effective particle volume associated with the water droplets trapped inside the fat droplets. Heat treatment increased the apparent shear viscosity of the emulsions, which was attributed to thermal denaturation and aggregation of the globular proteins altering the structure and interactions in the system. The strategies used to alter the microstructure and rheology of the multiple emulsions utilized in this study may be useful for the production of foods with novel textures or reduced-fat products. [Copyright &y& Elsevier]

Abstract: This study shows that controlled aggregation of acidic whey protein microspheres in water-in-oil (W/O) emulsions can be used to create semi-solid lipids. An acidic aqueous phase (10 g/100 g whey protein isolate, 100 mmol/L NaCl, pH 3.5) was homogenized with an oil phase (8 g/100 g polyglycerol polyricinoleate in soybean oil) to form a W/O emulsion. These emulsions were then heated (90 °C, 30 min) to induce thermal denaturation and gelation of the globular proteins in the internal aqueous phase, which promoted irreversible gelation of the overall W/O emulsion. Rheology measurements indicated that the resulting materials had paste-like properties: they were solid-like below a critical yield stress and fluid-like (shear-thinning) above this value. The apparent shear viscosity of the W/O emulsions increased with thermal treatment, aqueous phase content, and decreasing shear rate. W/O emulsion gels containing acidic aqueous phases were considerably weaker than those containing neutral aqueous phases due to the pH dependence of whey protein gelation. Controlled aggregation of whey protein microspheres within W/O emulsions may be a useful means of producing highly viscous or semi-solid lipids with low saturated and trans-fat contents for use in the food and other industries. [Copyright &y& Elsevier]

Abstract: The effect of heteroaggregation of oppositely charged protein microspheres dispersed within a liquid oil phase on the microstructure and rheological properties of water-in-oil (W/O) emulsions was evaluated. The aqueous phase of the initial W/O emulsions contained either 10% β-lactoglobulin or 10% lactoferrin (pH 7, 100mM NaCl). At this pH, β-lactoglobulin (BLG) is negatively charged while lactoferrin (LF) is positively charged. The oil phase consisted of a lipophilic non-ionic surfactant (8% polyglycerol polyricinoleate, PGPR) dispersed within soybean oil. Three 40% W/O emulsions were formed containing different types of protein microspheres: (i) BLG: 100% BLG droplets; (ii) LF: 100% LF droplets; and (iii) Mixed: 50% BLG droplets and 50% LF droplets. Prior to heating, the mixed emulsions had a higher shear viscosity, yield stress, and shear modulus than the BLG or LF emulsions, which suggested that electrostatic attraction led to the formation of a three-dimensional network of aggregated droplets. All three W/O emulsions underwent an irreversible fluid-to-solid transition when they were heated above ≈70°C. This phenomenon was attributed to thermal denaturation of the globular BLG and LF molecules within the aqueous phase promoting aggregation and network formation of the protein microspheres. After heating, the mixed emulsions had a higher shear viscosity, yield stress and shear modulus than the BLG or LF emulsions, suggesting that a stronger droplet network was formed due to electrostatic attraction. Shear rheology measurements of the W/O emulsions showed that the lipid phases formed after heating were non-ideal plastics characterized by a yield stress and shear thinning behavior. These results may facilitate the design of semi-solid or solid foods with reduced saturated- or trans-fat contents suitable for use in commercial products. [Copyright &y& Elsevier]

Abstract: Controlled aggregation of protein microspheres in water-in-oil (W/O) emulsions was used to form semi-solid lipid materials. The aqueous phase consisted of 10wt% whey protein isolate (WPI) in buffer solution (pH 7.0, 100mM NaCl). The oil phase consisted of a lipophilic nonionic surfactant (8 wt % polyglycerol polyricinoleate, PGPR) dispersed in a liquid oil (soybean oil). Lipid phases containing protein microspheres were formed by homogenization of the oil and aqueous phases to form a W/O emulsion followed by heating (90°C for 30min) to promote gelation of the WPI in the aqueous phase. Temperature-scanning dynamic shear measurements showed that the W/O emulsions underwent an irreversible liquid-to-solid transition when heated above the thermal denaturation temperature of WPI, which was attributed to protein gelation and microsphere aggregation. Optical microscopy indicated that a three-dimensional network of aggregated protein microspheres was formed at high aqueous phase contents (>30 wt %). Shear rheology measurements (shear stress versus shear rate) indicated that these structured emulsions were non-ideal plastic-like materials. The apparent shear viscosity increased with thermal treatment, increasing aqueous phase content, and decreasing shear rate. The structured W/O emulsions developed in this study may be useful materials for the development of foods with highly viscous or gel-like lipid phases, but low saturated or trans-fat contents. [Copyright &y& Elsevier]