1. Genetic and epigenetic X-chromosome variations in a parthenogenetic human embryonic stem cell line.
- Author
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Liu W, Yin Y, Jiang Y, Kou C, Luo Y, Huang S, Zheng Y, Li S, Li Q, Guo L, Gao S, and Sun X
- Subjects
- Cell Differentiation, Cell Line, DNA Fingerprinting, DNA Methylation, Down-Regulation, Genomic Imprinting, Humans, In Situ Hybridization, Fluorescence, Karyotyping, Oligonucleotide Array Sequence Analysis, Up-Regulation, Chromosomes, Human, X genetics, Embryonic Stem Cells cytology, Epigenomics methods, Parthenogenesis, X Chromosome Inactivation
- Abstract
Purpose: To assess the genetic and epigenetic status of parthenogenetic human embryonic stem cells (phESCs)., Methods: Cytogenetics, X chromosome inactivation (XCI) and gene expression patterns were analyzed in one phESC line (FY-phES-018) that was derived from our laboratory., Results: FY-phES-018 cells displayed the classical characteristics of normal hESCs. These cells had a 46, XX karyotype, and no inactive X chromosomes were observed before passage 20. After being cultured long term in vitro, some cells lost one X, and the proportion of cells with only one X gradually increased. At passage 35, almost all the cells displayed a 45, XO karyotype. Interestingly, at passage 45, the recovery of the X-chromosome was observed, and XCI became detectable; the mosaic ratio of 46, XX to 45, XO was 67:33. After passage 60, most cells displayed the 46, XX karyotype again with a mosaic ratio of 97:3. Some aberrant genomic imprinting was also observed in these cells., Conclusions: The phESCs line FY-phES-018 is both genetically and epigenetically unstable; therefore, further research is needed before using these cells.
- Published
- 2011
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