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PURPOSE: Oocyte competence is critical in success of assisted reproduction. Metabolic signaling between oocyte and cumulus cells within the cumulus-oocyte complex procure oocyte development. This study evaluated the relationship between respirometric activity of cumulus cells and maturity of corresponding oocytes. METHODS: In prospective cohort study, 20 women of age 28–42 undergoing IVF procedure were involved. To evaluate oocyte maturity, the cumulus cells from individual oocytes were assessed flow cytometrically by double labeling of cells with mitochondria specific dyes. The respirometric stress analysis using ATPase inhibitor oligomycin was applied to assess mitochondria metabolic abnormalities. RESULTS: The cumulus cells from each of 327 oocytes were analyzed. The respirometric index of cumulus cells (O′(R)) strongly correlates with maternal ovarian reserve, showing to be higher in patients with higher AMH (p

to determine if embryo banking with PGS is more optimal than proceeding with PGS regardless of embryo number.patients were divided into 2 groups, group 1 were those that banked embryos and proceeded through another round of IVF prior to PGS, and group 2 underwent PGS regardless of embryo number. Group 2 was divided into group 2A (patients with10 embryos) and group 2B (patients who had10 embryos).there was no difference in embryos biopsied, normal embryos, number transferred, and pregnancy rate between group 1 and 2. A significant number of patients did not have a transfer in group 2B (6/11) compared to group 1 (3/19) (P = 0.0419). There was no significance between pregnancy rates per transfer between group 1 (6/16) and group 2B (2/5).our data suggests that banking will increase the odds of going to transfer but there was no increase in pregnancy rates.

A laser is commonly used to remove a blastomere from an embryo for genetic testing. The laser uses intense heat which could possibly disrupt embryo development. It is the goal of this study to test the effects of different laser pulse lengths (and consequently heat) on the embryo biopsy procedure and embryo development. Each embryo biopsy was performed randomly utilizing laser pulse lengths of 0.604mS (group I), 0.708mS (group II), and 1.010mS (group III). For groups I, II, and III, 83, 86, and 71 embryos were biopsied, respectively. There was no difference in day 5 embryo quality or lysed blastomeres between groups. Average number of blastomeres biopsied between group I (1.0 ± 0.0), II (1.0 ± 0.2), and III (1.1 ± 0.2) was significant (0.0001). Our data demonstrates that laser pulse length does not influence the embryo biopsy procedure or embryo development.

This study retrospectively analysed array comparative genomic hybridization (CGH) results of 7753 embryos from 990 patients to determine the frequency of embryonic euploidy and its relationship with the cohort size (i.e. the number of embryos available for biopsy and array CGH analysis). Linear regression analysis was performed to assess the effect of cohort size on euploidy rate adjusted for the effect of female age. While increasing female age was associated with a significant decrease in euploidy rate of day-3 and day-5 embryos (P < 0.001 for both groups), cohort size was not significantly associated with euploidy rate. Logistic regres- sion analysis was performed to assess the effect of cohort size, adjusted for maternal age, on the likelihood of having at least one euploid embryo available for transfer. The odds of having at least one euploid embryo in an assisted cycle was significantly decreased by increasing female age (P < 0.01 for both day-3 and day-5 embryos) and was significantly increased by every additional embryo available for analysis (P < 0.001 for both day-3 and day-5 embryos). RBMOnline

Objective To report a clinical pregnancy after rebiopsy and vitrification of blastocysts following allele dropout (ADO) of biopsied day 3 embryos. Design Case report. Setting Private center. Patient(s) Thirty-year-old woman and her 33-year-old husband who carries the single-gene condition paraganglioma. Intervention(s) In vitro fertilization with day 3 embryo biopsy–ET–blastocyst biopsy and vitrification–subsequent frozen ET cycle. Main Outcome Measure(s) Results from preimplantation genetic diagnosis and pregnancy results after fresh and frozen ETs. Result(s) Nineteen oocytes were retrieved of which 13 were mature and 12 fertilized. Eleven embryos were biopsied on day 3: two were normal, five were affected, and four exhibited ADO. The two normal blastocysts were transferred, and three of the ADO blastocysts were biopsied and sent for reanalysis. The biopsied blastocysts were vitrified. No pregnancy resulted from the fresh ET. One of the biopsied blastocysts was normal, one received no result, and one exhibited ADO. A singleton clinical pregnancy resulted from a subsequent frozen ET of the thawed biopsied normal blastocyst. Conclusion(s) Rebiopsy and vitrification of blastocysts could be used in cases of ADO or lack of results after day 3 embryo biopsy.

OBJECTIVE: To determine if vitrification (Vit) can provide improved survival and pregnancy rates compared to a modified slow freeze protocol that had proven very successful for 2PN and multi-cell embryos over the years. DESIGN: Prospective comparison with Vit. performed 3 set days each week vs. 4 for slow freeze. MATERIALS AND METHODS: Vit. protocol: Irvine media, 6-10 minutes in equilibration solution and 90 seconds in vitrification solution at room temperature then loaded onto High security vitrification (HSV) highsecurity straw in

blastocyst biopsy. The practice of biopsying all non-arrested D5 embryos provides the opportunity to have genetic information on all embryos eligible for fresh transfer on D6, potentially adding to the pool of embryos available for transfer. Biopsy of non-arrested D5 embryos may also avoid the need to cryopreserve developmentally competent embryos without ploidy information at the time of transfer.

Purpose A laser is commonly used to remove a blastomere from an embryo for genetic testing. The laser uses intense heat which could possibly disrupt embryo development. It is the goal of this study to test the effects of different laser pulse lengths (and consequently heat) on the embryo biopsy procedure and embryo development. Methods Each embryo biopsy was performed randomly utilizing laser pulse lengths of 0.604mS (group I), 0.708mS (group II), and 1.010mS (group III). Results For groups I, II, and III, 83, 86, and 71 embryos were biopsied, respectively. There was no difference in day 5 embryoqualityorlysedblastomeresbetweengroups.Average number of blastomeres biopsied between group I (1.0±0.0), II (1.0±0.2), and III (1.1±0.2) was significant (0.0001). Conclusion Our data demonstrates that laser pulse length does not influence the embryo biopsy procedure or embryo development.