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Your search keyword '"Richardson, Lucy"' showing total 3 results
Start Over Author "Richardson, Lucy" Topic embryo, mammalian
3 results on '"Richardson, Lucy"'

1. Distinct pathways drive anterior hypoblast specification in the implanting human embryo.

Author

Weatherbee BAT, Weberling A, Gantner CW, Iwamoto-Stohl LK, Barnikel Z, Barrie A, Campbell A, Cunningham P, Drezet C, Efstathiou P, Fishel S, Vindel SG, Lockwood M, Oakley R, Pretty C, Chowdhury N, Richardson L, Mania A, Weavers L, Christie L, Elder K, Snell P, and Zernicka-Goetz M

Subjects
Pregnancy, Female, Humans, Embryonic Development genetics, Signal Transduction, Embryo Implantation, Germ Layers, Embryo, Mammalian metabolism
Abstract

Development requires coordinated interactions between the epiblast, which generates the embryo proper; the trophectoderm, which generates the placenta; and the hypoblast, which forms both the anterior signalling centre and the yolk sac. These interactions remain poorly understood in human embryogenesis because mechanistic studies have only recently become possible. Here we examine signalling interactions post-implantation using human embryos and stem cell models of the epiblast and hypoblast. We find anterior hypoblast specification is NODAL dependent, as in the mouse. However, while BMP inhibits anterior signalling centre specification in the mouse, it is essential for its maintenance in human. We also find contrasting requirements for BMP in the naive pre-implantation epiblast of mouse and human embryos. Finally, we show that NOTCH signalling is important for human epiblast survival. Our findings of conserved and species-specific factors that drive these early stages of embryonic development highlight the strengths of comparative species studies., (© 2024. The Author(s).)

Published
2024
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2. A single cell characterisation of human embryogenesis identifies pluripotency transitions and putative anterior hypoblast centre

Author

Molè, Matteo A, Coorens, Tim HH, Shahbazi, Marta N, Weberling, Antonia, Weatherbee, Bailey AT, Gantner, Carlos W, Sancho-Serra, Carmen, Richardson, Lucy, Drinkwater, Abbie, Syed, Najma, Engley, Stephanie, Snell, Philip, Christie, Leila, Elder, Kay, Campbell, Alison, Fishel, Simon, Behjati, Sam, Vento-Tormo, Roser, and Zernicka-Goetz, Magdalena

Subjects
animal structures, Nodal Protein, Gastrulation, Embryonic Development, Gene Expression Regulation, Developmental, Embryo, Mammalian, 3. Good health, Bone Morphogenetic Protein 1, Fibroblast Growth Factors, Spatio-Temporal Analysis, Multigene Family, embryonic structures, Image Processing, Computer-Assisted, Humans, Cell Lineage, Embryo Implantation, RNA-Seq, Single-Cell Analysis, Wnt Signaling Pathway, Cells, Cultured, Germ Layers
Abstract

Following implantation, the human embryo undergoes major morphogenetic transformations that establish the future body plan. While the molecular events underpinning this process are established in mice, they remain unknown in humans. Here we characterise key events of human embryo morphogenesis, in the period between implantation and gastrulation, using single-cell analyses and functional studies. First, the embryonic epiblast cells transition through different pluripotent states and act as a source of FGF signals that ensure proliferation of both embryonic and extra-embryonic tissues. In a subset of embryos, we identify a group of asymmetrically positioned extra-embryonic hypoblast cells expressing inhibitors of BMP, NODAL and WNT signalling pathways. We suggest that this group of cells can act as the anterior singalling centre to pattern the epiblast. These results provide insights into pluripotency state transitions, the role of FGF signalling and the specification of anterior-posterior axis during human embryo development.

3. A single cell characterisation of human embryogenesis identifies pluripotency transitions and putative anterior hypoblast centre

Author

Molè, Matteo A, Coorens, Tim H H, Shahbazi, Marta N, Weberling, Antonia, Weatherbee, Bailey A T, Gantner, Carlos W, Sancho-Serra, Carmen, Richardson, Lucy, Drinkwater, Abbie, Syed, Najma, Engley, Stephanie, Snell, Philip, Christie, Leila, Elder, Kay, Campbell, Alison, Fishel, Simon, Behjati, Sam, Vento-Tormo, Roser, and Zernicka-Goetz, Magdalena

Subjects
animal structures, Nodal Protein, Gastrulation, Gene Expression Regulation, Developmental, Embryonic Development, Embryo, Mammalian, 3. Good health, Bone Morphogenetic Protein 1, Fibroblast Growth Factors, Spatio-Temporal Analysis, Multigene Family, embryonic structures, Image Processing, Computer-Assisted, Humans, Cell Lineage, Embryo Implantation, RNA-Seq, Single-Cell Analysis, Wnt Signaling Pathway, Cells, Cultured, Germ Layers
Abstract

Following implantation, the human embryo undergoes major morphogenetic transformations that establish the future body plan. While the molecular events underpinning this process are established in mice, they remain unknown in humans. Here we characterise key events of human embryo morphogenesis, in the period between implantation and gastrulation, using single-cell analyses and functional studies. First, the embryonic epiblast cells transition through different pluripotent states and act as a source of FGF signals that ensure proliferation of both embryonic and extra-embryonic tissues. In a subset of embryos, we identify a group of asymmetrically positioned extra-embryonic hypoblast cells expressing inhibitors of BMP, NODAL and WNT signalling pathways. We suggest that this group of cells can act as the anterior singalling centre to pattern the epiblast. These results provide insights into pluripotency state transitions, the role of FGF signalling and the specification of anterior-posterior axis during human embryo development.

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