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Poly (3-hydroxybutyrate) and poly (ethylene glycol) are frequently used as materials for designing scaffolds in tissue engineering. This work aimed the production of P3HB-PEG scaffolds in different weight ratio of PEG using electrospinning and characterized. Samples also were examined for rabbit chondrocyte cell assay. 5 wt% of PEG decreased the nanofiber diameter with more uniformity and enhanced hydrophilicity. The biodegradation rate increased while the mechanical features tended to decrease. In vitro tests showed good chondrocyte cell viability and adhesion. These results manifested that electrospun the P3HB-PEG scaffolds could enhance cartilage regeneration, showing their marvelous prospect as scaffolds for cartilage tissue engineering. [ABSTRACT FROM AUTHOR]

The aim of this study is to gain the best structure of Poly (3-hydroxybutyrate) (PHB)/chitosan electrospun aligned and random fibrous scaffold for nerve tissue engineering applications using electrospinning method, morphologically and biologically. The results illustrated that by addition of chitosan, surface porosity percentages have increased while the water contact angle has decreased, for both structures. Results of biodegradation rate noted that aligned PHB/chitosan fibrous scaffolds had a slower degradation rate than random PHB/chitosan fibrous scaffolds. Finally, cultivation results of rat neuronal-like cells (B65 cell line) on electrospun-aligned and random fibrous scaffolds were compared. Results of MTT assay demonstrated better improvement for the proliferation of B65 cells on electrospun-aligned PHB/chitosan scaffolds. Also, SEM images indicated that the bi-polar neurite extensions and the orientation of nerve cells were aligned in the direction of the fibre alignment. [ABSTRACT FROM AUTHOR]

3-D nanocomposite scaffolds have recently had wide applications in bone tissue engineering. In this study, 58S bioactive glass nanoparticles (nBGs) were synthetized using sol-gel method and characterized. According to the results, the size of nanoparticles was achieved less than 100 nm. In the following step, Poly (3-hydroxybutyrate) (P3HB) was reinforced with 7.5, 10 and 15 %wt. of nBGs. Then, nanocomposite scaffolds were prepared by electrospinning technique and the structure was characterized structurally and mechanically. The results show that nanocomposite scaffolds have interconnected porosity with proper distribution and interaction of nBGs with polymeric nanofibres. Finally, The bioactivity of the optimized scaffold was evaluated by soaking in the simulated body fluid for 21 days and results obtained the P3HB/nBG electrospun scaffolds are bioactive as the apatite layer was observed on the surface of the fibres. To sum up, P3HB/nBG electrospun scaffold could be a very good candidate for bone tissue engineering applications. [ABSTRACT FROM AUTHOR]

In this investigation, a nanofibrous scaffold was fabricated through electrospinning of polycaprolactone (PCL) and chitosan (CS) using a novel collector to make better orientation and pore size for cell infiltration. PCL/CS nanofibers with 90-rpm collector speed and 40° angle between collector wires of the new collector have fewer diameters with better pore, size and nanofibers orientation. Mechanical properties, roughness parameters, topology, structure, hydrophilicity, and cell growing were considered for liver tissue engineering. The cell culture was done using epithelial liver mouse cells. The developed electrospun PCL/CS scaffold using novel collector would be an excellent matrix for biomedical applications especially liver tissue engineering. [ABSTRACT FROM PUBLISHER]

A novel biodegradable nano/micro hybrid structure was obtained by electrospinning P3HB or PCL nanofibers onto a twisted silk fibroin (SF) structure, with the aim of fabricating a suitable scaffold for tendon and ligament tissue engineering. The electrospinning (ES) processing parameters for P3HB and PCL were optimized on 2D samples, and applied to produce two different nano/micro hybrid constructs (SF/ES-PCL and SF/ES-P3HB). Morphological, chemico-physical and mechanical properties of the novel hybrid scaffolds were evaluated by SEM, ATR FT-IR, DSC, tensile and thermodynamic mechanical tests. The results demonstrated that the nanofibers were tightly wrapped around the silk filaments, and the crystallinity of the SF twisted yarns was not influenced by the presence of the electrospun polymers. The slightly higher mechanical properties of the hybrid constructs confirmed an increase of internal forces due to the interaction between nano and micro components. Cell culture tests with L929 fibroblasts, in the presence of the sample eluates or in direct contact with the hybrid structures, showed no cytotoxic effects and a good level of cytocompatibility of the nano/micro hybrid structures in term of cell viability, particularly at day 1. Cell viability onto the nano/micro hybrid structures decreased from the first to the third day of culture when compared with the control culture plastic, but appeared to be higher when compared with the uncoated SF yarns. Although additional in vitro and in vivo tests are needed, the original fabrication method here described appears promising for scaffolds suitable for tendon and ligament tissue engineering. [ABSTRACT FROM AUTHOR]