1. The evolution of two-dimensional gel electrophoresis - from proteomics to emerging alternative applications.
- Author
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Lee PY, Saraygord-Afshari N, and Low TY
- Subjects
- Electrophoresis, Gel, Two-Dimensional history, Electrophoresis, Polyacrylamide Gel trends, History, 20th Century, History, 21st Century, Mass Spectrometry, Protein Processing, Post-Translational, Proteome, Electrophoresis, Gel, Two-Dimensional trends, Proteomics methods
- Abstract
Two-dimensional gel electrophoresis (2-DE) is a technique that has been widely applied in a variety of proteomics studies. It is capable of resolving complex protein mixtures into individual protein spots based on their isoelectric point and molecular weight, enabling large-scale analysis of protein expression patterns for deciphering their changes in different biological conditions. 2-DE is a powerful tool that empowers researchers to perform differential qualitative and quantitative proteome analysis and is particularly advantageous for characterizing protein isoforms and post-translationally modified proteins. Despite its popularity as the workhorse for proteomics in the past few decades, it has been gradually displaced by the more sophisticated and high-performance mass spectrometry-based methods. However, there are several variations of the 2-DE technique that have emerged as promising approaches that shine new light on specific niches that 2-DE could still contribute. In this review, we first provide an overview of the applications of 2-DE, its merits and pitfalls in the current proteomic research arena, followed by a discussion on several alternative approaches for potential future applications., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2019 Elsevier B.V. All rights reserved.)
- Published
- 2020
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