1. Fabrication of an electrode-viologen-hydrogenase heterogeneous system and the electrochemical hydrogen evolution.
- Author
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Qian DJ, Nakamura C, Noda K, Zorin NA, and Miyake J
- Subjects
- Electrochemistry methods, Potentiometry, Thiocapsa enzymology, Tin Compounds, Electrochemistry instrumentation, Enzymes, Immobilized metabolism, Hydrogen, Hydrogenase metabolism
- Abstract
An indium tin oxide (ITO) electrode was chemically modified by one layer of viologen (VIO) derivative, which possessed a persistent and reproducible electrochemical response. A monolayer of a thermal stable hydrogenase from Thiocapsa roseopersicina was stabilized on a synthesized poly-L-lysine subphase surface and transferred onto the electrode for fabrication of an ITO-VIO-hydrogenase heterogeneous system. Electrochemical properties of both the ITO-VIO monolayer and the heterogeneous ITO-VIO-hydrogenase system have been investigated. Hydrogen evolution could be measured by potentiostating the VIO-hydrogenase-covered ITO electrode to "electroplate" [(VIO+)n]surf, and a large increase in hydrogen evolution was observed when using an electrolyte solution containing sodium dithionite. We discuss the possible electron transfer process.
- Published
- 2000
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