1. Nanogold-actuated biomimetic peroxidase for sensitized electrochemical immunoassay of carcinoembryonic antigen in human serum
- Author
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Chen, Huafeng, Tang, Juan, Su, Biling, Chen, Guonan, Huang, Jianxin, and Tang, Dianping
- Subjects
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BIOMIMETIC chemicals , *NANOPARTICLES , *COLLOIDAL gold , *PEROXIDASE , *CARCINOEMBRYONIC antigen , *PRUSSIAN blue , *IMMUNOASSAY , *ELECTROCHEMISTRY - Abstract
Abstract: A new electrochemical immunoassay protocol for the determination of carcinoembryonic antigen (CEA) in human serum was developed by means of immobilizing horseradish peroxidase-labeled anti-CEA antibodies (HRP-anti-CEA) on the nanogold/nano-Fe3O4 particles-functionalized polyion complex (PIC) membrane. With a one-step immunoassay format, the formed immunocomplex inhibited partly the bioactive center of the immobilized HRP, and decreased the HRP toward the reduction of H2O2. Nanogold-actuated nano-Fe3O4 mimetic peroxidase was used for the sensitivity improvement of the electrochemical immunosensor with Prussian blue (PB) as mediators. Under optimal conditions, the electrochemical immunosensor displayed a wide dynamic range of 0.1–220ngmL−1 with a relative low detection limit (LOD) of 10pgmL−1 CEA (at 3σ). Intra- and inter-assay show good precisions with a coefficient of variation (CV) lower than 7.8% and 7.3%, respectively. No significant difference at the 95% confidence level was encountered in the analysis of 17 human serum specimens between the electrochemical immunosensor and automatic electrochemiluminescent (ECL) analyzer for the determination of CEA. [ABSTRACT FROM AUTHOR]
- Published
- 2010
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