1. The effect of electroacupuncture at ST36 on severe thermal injury-induced remote acute lung injury in rats.
- Author
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Song XM, Wu XJ, Li JG, Le LL, Liang H, Xu Y, Zhang ZZ, and Wang YL
- Subjects
- Acute Lung Injury etiology, Analysis of Variance, Animals, Biomarkers metabolism, Burns metabolism, Burns pathology, Cytokines metabolism, Disease Models, Animal, HMGB1 Protein metabolism, Inflammation metabolism, Lung metabolism, Lung ultrastructure, Male, RNA, Messenger metabolism, Random Allocation, Rats, Rats, Sprague-Dawley, Acupuncture Points, Acute Lung Injury therapy, Burns therapy, Electroacupuncture
- Abstract
Objective: Acupuncture at ST36 can produce anti-inflammatory effects, which might be associated with vagus nerve activity. This study explored the effects of electroacupuncture (EA) at ST36 on severe thermal injury-induced remote acute lung injury in rats., Interventions: Forty male Sprague-Dawley (SD) rats were randomly divided into five groups: (1) the sham (S) group, (2) the thermal injury (TEM) group subjected to 30% total body surface area (30% TBSA) third-degree scald, (3) the EA at ST36 group subjected to EA stimulation at ST36 (3V, 2ms, and 3Hz) after 30% TBSA scald, (4) the EA at non-acupoint group subjected to EA stimulation at non-acupoint after 30% TBSA scald, and (5) the α-bungarotoxin (α7 nicotinic acetylcholine receptor subunit antagonist) group administered 1.0 μg kg(-1) α-bungarotoxin before EA at ST36., Measurements and Main Results: Thermal injury of 30% TBSA induced leukocytosis in the alveolar space, interstitial edema, and the pro-inflammatory cytokines interleukin (IL)-1β, IL-6, and high-mobility group box 1 (HMGB-1); the expression of both HMGB-1 messenger RNA (mRNA) and protein in lung tissue was significantly enhanced. EA at ST36 significantly downregulated the levels of inflammatory cytokines and improved lung tissue injury. However, pretreatment with α-bungarotoxin reversed the effects of electrical stimulation of ST36., Conclusions: EA at ST36 might have a potential protective effect on severe thermal injury-induced remote acute lung injury via limitation of inflammatory responses in rats., (Copyright © 2015 Elsevier Ltd and ISBI. All rights reserved.)
- Published
- 2015
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