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This work describes crucial steps LED lighting from a simple device microbial fuel cell without membrane, using a domestic wastewater. Firstly, wastewater from activated sludge of Beni Messous was analyzed to be inoculated in the MFC as a source of anodic electroactive biofilm AEB using acetate as substrate. The AEB revealed higher current densities exceeding 100 mA/m2, where, working electrode (WE) polarized at +0.155 V/SCE had given better result compared to −0.155/SCE and +0.3 V/SCE. The microbial analyses had demonstrated a microbial population diversify belonging to bacterial groups already studied and listed in the literature to be electroactive and that Gram negative bacteria were dominated more than Gram positive. Secondly, the performances of a membrane less MFC (ML-MFC) were investigated polarization curves study and demonstrated that ML-MFC had a reactor behavior to produce current. After 150 days of ML-MFC operation, the maximum power and current densities had reached 7.07 and 50 mA/m2, respectively, with an internal resistance of 434 Ω. Through the present study, using Benimessous-STEP wastewater for the renewable energy (bioelectricity) production via a ML-MFC was considered as a feasible, economical, and sustainable process. [ABSTRACT FROM AUTHOR]