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Background: Mitral valve failure can require repair or replacement. Replacement bioprosthetic valves are treated with glutaraldehyde prior to implantation. The aim of this study was to determine the changes in mechanical properties following glutaraldehyde fixation of mitral valve chordae., Methods: To investigate the impact of glutaraldehyde on mitral valve chordae, 24 basal chordae were dissected from four porcine hearts. Anterior and posterior basal (including strut) chordae were used. All 24 chordae were subjected to a sinusoidally varying load (mean level 2N, dynamic amplitude 2N) over a frequency range of 0.5-10 Hz before and after glutaraldehyde treatment., Results: The storage and loss modulus of all chordal types decreased following glutaraldehyde fixation. The storage modulus ranged from: 108 to 119 MPa before fixation and 67.3-87.4 MPa following fixation for basal chordae; 52.3-58.4 MPa before fixation and 47.9-53.5 MPa following fixation for strut chordae. Similarly, the loss modulus ranged from: 5.47 to 6.25 MPa before fixation and 3.63-4.94 MPa following fixation for basal chordae; 2.60-2.97 MPa before fixation and 2.31-2.93 MPa following fixation for strut chordae., Conclusion: The viscoelastic properties of mitral valve chordae are affected by glutaraldehyde fixation; in particular, the reduction in storage moduli decreased with an increase in chordal diameter.

The aim of this study was to determine the regional variation in viscoelastic properties of mitral valve leaflets over a range of physiological and patho-physiological frequencies. This included comparisons to be made between anterior and posterior leaflets, anterior leaflet clear and rough zones, and radial and circumferential leaflet orientation. Dynamic mechanical analysis was used to determine frequency-dependent viscoelastic properties. The valve leaflets were dissected from eight porcine hearts. The leaflets were loaded under a sinusoidal tensile displacement, with a mean dynamic peak to trough strain of 11%, applied to all leaflet samples at nine different frequencies, ranging from 0.5 to 10 Hz. The anterior leaflet has higher storage and loss stiffness than the posterior leaflet. The storage stiffness of circumferential tissue is greater than that of radially oriented valve tissue (2.0 ± 1.6 N/mm cf. 1.7 ± 0.9 N/mm; p < 0.05); however, the loss stiffness is greater for radial tissue (0.15 ± 0.07 cf. 0.14 ± 0.09 N/mm; p < 0.05). Likewise, the storage stiffness of the anterior leaflet clear zone is greater than that of the rough zone (2.4 ± 1.6 cf. 2.1 ± 1.2; p < 0.05), but the loss stiffness is greater for the rough zone (0.17 ± 0.09 N/mm cf. 0.14 ± 0.08 N/mm; p < 0.05). In conclusion, the viscoelastic properties of porcine mitral valve leaflets have regional variations, with dynamic stiffness being dependent on circumferential or radial orientation and on location at a clear or rough zones.

This study aimed to characterise viscoelastic properties of different categories of chordae tendineae over a range of frequencies. Dynamic Mechanical Analysis (DMA) was performed using a materials testing machine. Chordae (n=51) were dissected from seven porcine hearts and categorised as basal, marginal, strut or commissural. Chordae were loaded under a sinusoidally varying tensile load at a range of frequencies between 0.5 and 5Hz, both at a standardised load (i.e. same mean load of 4N for all chordae) and under chordal specific loading (i.e. based on in vivo loads for different chordae). Storage modulus and stiffness were frequency-dependent. Loss modulus and stiffness were frequency-independent. Storage and loss moduli, but not stiffness, decreased with chordal diameter. Therefore, strut chordae have the lowest moduli and marginal chordae the highest moduli. The hierarchy of dynamic storage and loss moduli is: marginal, commissural, basal and strut. In conclusion, viscoelastic properties of chordae are dependent on both frequency and chordal type. Future/novel replacement chordal materials should account for frequency and diameter dependent viscoelastic properties of chordae tendineae., (© 2013 Published by Elsevier Ltd.)