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The present study was conducted to find out the effectiveness of planned teaching programme (PTP) on prevention of gestational diabetes mellitus (GDM) among antenatal mothers in selected PHCs at Mangalore. The purpose of this study to educate the antenatal mothers to take care of themselves at home and day to day activities to maintain stranger vigilance for early signs of GDM and its complications and seek medical aid immediately. However, there is lack of knowledge and practice regarding the preventive measures of GDM. This study is aimed at improving the knowledge of antenatal mothers on prevention of GDM. An evaluatory research approach with one group pre-test post test design was used for this study. The sample consisted of 50 antenatal mothers. Samples are selected by purposive sampling technique. The study was conducted in PHCs and Lady Goschen Hospital at Mangalore. Data were collected by administering structured interview schedule prior and after the administration of planned teaching programme. The collected data were analyzed by using descriptive and inferential statistics. Conceptual framework based on Rosenstoch’s (1974) and Becker and Maiman’s (1975) health belief model was used in this study.The study findings revealed that knowledge gained through PTP was good, as it was evident with high significance (t49=22.8547, p1= 18) and pre-test (X2= 27) knowledge score. Area-wise pre-test and post-test knowledge score of antenatal mothers shows higher mean percentage score (92%) in the area of prevention and least mean percentage score (87%) in the area GDM. Paired‘t’ test was used for the area wise comparison between pre-test and post-test knowledge score on prevention of GDM and was found to be highly significant. Chi-square was computed to test the association between pre-test knowledge score and selected demographic variables of antenatal mothers. However there was no significant association between the pre-test knowledge score and selected demographic variables like age, education, occupation, income and gravid at 0.05 level of significance. PTP is given through flip chart, charts, Power-Point and demonstration was found for an effective strategy for providing information and for improving the knowledge of antenatal mothers, and prevention of GDM. It was well appreciated and accepted by the antenatal mothers. The result of the study showed great need for health personnel to educate the antenatal mothers regarding prevention and promotion of health.