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The orientation of fish in a horizontal plane has important consequences for estimating their true size from horizontal acoustic records. The aim of this work was to verify the assumption that during the daytime fish are randomly orientated in the lacustrine zone of the canyon-shaped Řimov reservoir and to compare distributions of fish orientation between the lacustrine and tributary (riverine) zones. Fish orientation was acoustically surveyed at fixed locations using the SIMRAD EK 60 split-beam echo sounder (elliptical beam, 120 kHz) with a horizontally aligned transducer. The horizontal aspect (angle between the fish body and the transducer axis) was used to describe their orientation. The conventional single-echo detector (SED) and the cross-filter detector (CFD) were applied. No trend was found along the reservoir when comparing records from four sites processed with the conventional SED. At all sites, most fish appeared to move predominantly in directions perpendicular to the central axis of the acoustic beam, i.e. the side-aspects (90°) of fish prevailed over other aspects. The CFD registered tracked fish several times more often than the SED. In the lacustrine zone the frequency distribution of fish aspect appears very similar when recorded by sonar beams oriented parallel to and across the longitudinal axis of the reservoir (criss-cross-beaming experiment). In the tributary zone, beaming perpendicular to the longitudinal axis of the reservoir revealed a significantly higher proportion of fish moving along the longitudinal axis. Therefore, the assumption of random fish orientation is not applicable in the tributary zones of such reservoirs.

The aim of this study was to quantify the efficiency of different lengths of beach seine nets for each diel period in freshwater reservoirs. Only fish older than young-of-the-year were considered. Nets of 10, 20 and 50 m length ("in-nets") were tested in an enclosed area framed by a 200 m long net (block net). The net efficiency estimate was calculated as the ratio of fish catches with the in-net and block net divided by the ratio of their respective areas. The net efficiency estimate was significantly different between day and night catches. At night, the efficiency estimate of nets depended on the size of the fish. The efficiency estimate of a 10 m long net decreased significantly in reverse correlation with fish size. A similar trend was found when using a 20 m long net. The efficiency estimate of a 50 m long net was independent of fish size. The variance in efficiency estimate between samples with a given net length was high, but decreased with longer nets. Of five species tested, only the efficiency estimate for catching bream (Abramis brama) increased significantly with the length of net. The biomass and abundance of larger fish was generally higher at night, although especially short nets exhibited a spuriously high efficiency estimate during the day, probably due to the concentrating (chasing) effect of the hauling ropes. We therefore recommend the use of a 50 m long net, since its nighttime efficiency estimate was about 0.9 in terms of both sampling abundance and biomass. A model relating the efficiency estimate and net length was developed with the data acquired.

In August 2004 and 2005, an extensive study of the fish community was carried out in the largest water supply reservoir in the Czech Republic and Central Europe, the canyon-shaped Želivka Reservoir, using a fleet of Nordic multimesh gillnets. Fishes were sampled at eight locations along the longitudinal profile of the reservoir and at five benthic depth layers covering depths from the surface down to 18 m (benthic gillnet 1·5 m high), and at three pelagic depth layers down to the depth of 5 m above the bottom (pelagic gillnets 4·5 m high). Catches of both juvenile (age 0+ year) and adult (fishes >1 year) fishes were highest in the upper layers of the water column (i.e. in the epilimnion down to 5 m, and down to 10 m in the benthic habitats). Along the tributary–dam axis in the pelagic habitats, both juvenile and adult fishes preferred the upper part of the reservoir, where the maximum number of species and also the greatest abundance of zooplankton were found. In the benthic habitats, fishes selected location according to factors other than trophic status. More juvenile fishes were recorded in the benthic habitats than in the pelagic habitats. Depth had the largest explanatory power for predicting fish community composition, followed by the affiliation with benthic and pelagic habitats, and location on the longitudinal axis of the reservoir. The fish community was represented mainly by cyprinids and consisted of two distinct groups of species, with bleak Alburnus alburnus, rudd Scardinius erythrophthalmus and asp Aspius aspius dominating the offshore group while perch Perca fluviatilis and ruffe Gymnocephalus cernuus were affiliated with the inshore group of the adult fish community. Roach Rutilus rutilus, bream Abramis brama and pikeperch Sander lucioperca occurred in important proportions in both the inshore and the offshore zones. All species, with the exceptions of adult perch (1+ year and older), 0+ year perch and 0+ year roach, preferred the most eutrophic tributary part of the reservoir. The fish community was relatively stable between the 2 years sampled.

Spatial distributions of fish were studied in two types of reservoirs by means of hydroacoustics during the summer. Different patterns of fish distribution were found in non-cascade (Řimov, Želivka) and cascade (Kamýk, Slapy, Stěchovice, Vrane) reservoirs. Maximum biomass and density of fish were observed in the tributary area of the non-cascade reservoir. The biomass declined towards the dam area. Average weight of fish showed the opposite trend—maximum average weight was observed in the dam area and declined towards the tributary area. In the cascade reservoirs fish distribution was found to be more complicated but the maximum biomass and density of fish were observed in the dam areas, whereas the tributary areas were nearly fishless. Poor ecohydrological conditions in the tributaries of cascade reservoirs are likely to be responsible for the low abundance of fish in these areas. This was evident in the example of Vrane reservoir which has two major inflows. Low biomass and density of fish were observed in the cold and low oxygen saturated inflowing cascade Vltava River while higher biomass and density were observed in the warm and well oxygen saturated Sazava River inflow. Average weights of fish followed the same trend as in non-cascade reservoirs—the maximum was in the dam area and it declined towards the tributary area. The vertical distribution of fish is driven by the fully developed stratification of temperature and oxygen during the summer season in non-cascade reservoirs. A significantly lower biomass of fish was observed below the thermocline in vertical surveys in both non-cascade and cascade reservoirs. Summer stratification in cascade reservoirs is weakly developed or is not developed at all due to hypolimnetic releases of cold water and short retention times. High transparency suggests lower primary production in cascade reservoirs.