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The sea cucumber Holothuria arenacava was discovered in the Mombasa marine reserve in 1997 and described by Samyn et al. (2001). The reproductive biology of this holothurian was investigated in order to (1) characterize the reproductive pattern, (2) examine the relationship among environmental parameters including temperature, light and lunar period, and (3) examine the relationship between the reproductive pattern and feeding of this new species. The gonad index method and microscopic examination of gonads was used to analyze samples collected for a period of 13 months. H. arenacava displayed an annual reproductive cycle with gametogenesis commencing in July during the south-east monsoons, when temperature and light intensity are lowest along the Kenyan coast. Gonad growth peaked in February–March at the end of the north-east monsoons when temperatures and light reach their annual maxima along the Kenyan coast. The higher correlation between light intensity and gonad growth (r=93) than temperature (r=0.71), coupled with the fact that temperatures continued to drop for a month after gametogenesis had already commenced, suggests that light intensity and not temperature is the cue for the onset of gametogenesis in this species. Spawning was synchronized between females and males and occurred during a short period between March and May (inter-monsoonal period) when both temperature and light intensity decrease along the Kenyan coast. Male and female gonad indices showed significant variation with lunar day and no lunar periodicity was observed in this sea cucumber. The sex ratio of the population of H. arenacava was skewed towards significantly more females than males, and females were significantly larger and had larger gonads and gonad indices than males. These life history strategies including spawning during a short discrete period, more and larger females that have larger gonads (i.e., typically more fecund), and spawning just prior to the peak in phytoplankton concentrations, a time that is probably more favorable for larval development, may serve to increase the reproductive success of this sea cucumber.

Reproduction in the widely distributed tropical sea urchin Echinometra mathaei (de Blainville) was examined in three Kenyan reef lagoons that differed in substrate cover and E. mathaei population density. Histological examination of the gonads and gonad index measurements for 3 years showed a seasonal reproductive cycle with gametogenesis commencing in July, when temperature and light are at their lowest, and spawning commencing in December and peaking in February to May, when temperature and light reach their annual maxima. Monthly gonad indices correlated significantly with seawater temperature and light intensity. Male urchins had mature gametes for a longer period (8 months) than females (6 months), possibly an adaptive strategy that increases the probability of fertilization. Lunar periodicity was not observed, as male and female gonads were full of gametes on all days of the lunar cycle during the period of spawning. The peak in spawning activity coincides with the peak in phytoplankton abundance, which could ensure high food availability for the planktonic larvae. Gonad weights were significantly higher relative to urchin weight at the reef having the largest mean sizes and lowest population of urchins, indicating availability of food resources for growth and reproduction. E. mathaei at the reef with the highest density had the smallest urchins and high relative gonad sizes, indicative of a tradeoff between growth and reproduction when food is limited.

The sympatric echinoids Diadema savignyi and D. setosum coexist in shallow reef lagoons throughout East Africa. The reproductive strategies of these echinoids were studied to investigate reproductive isolation as a possible mechanism for maintaining the coexistence of these closely related species. The annual reproductive cycle and lunar periodicity were determined by gonad index measurements, histological examination of gametogenesis, and induction of spawning with injections of KCl. The peak reproductive period of D. savignyi coincided with the north-east monsoon period (when light and temperatures are high) as gonad indices were high (>8%) beginning in February and peaked at 9.7% in May. Gonad indices subsequently rapidly decreased (by 26%) in June at the beginning of the cooler south-east monsoon period. However, the presence of sperm and ova in most months of the year indicates continuous gametogenesis with reduced reproductive effort during the cooler months. The annual cycle for D. setosum showed less of a seasonal trend as gonad indices remained above 7% throughout much of the year but tended to be highest when temperatures were lower. This is the first confirmation of continuous reproduction in these two species at the equator. The reproductive patterns of both species remained consistent over 2 years of sampling. Both species exhibited a synchronized lunar spawning periodicity during the 3 months sampled, with D. setosum spawning on lunar days 8–10 and D. savignyi spawning after the full moon (lunar days 17–18). Whereas spawning in D. savignyi was very tightly synchronized, 20% of D. setosum individuals still spawned after the peak spawning period. The coexistence of these closely related species appears to be maintained by temporal reproductive isolation during the lunar spawning period reinforced by seasonal differences in reproductive effort.

ummary A comparison of Echinometra mathaei densities and sizes was undertaken on an inner reef lagoon and an outer reef edge on a densely populated tourist beach at Diani, Kenya. Echinometra mathaei densities and average lengths were significantly higher (t-test, P