1. The Thermoperiod Alters Boper Gene Expression and Thereby Regulates the Eclosion Rhythm of Bradysia odoriphaga (Diptera: Sciaridae)
- Author
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Guo Zhaojiang, Qingjun Wu, Yuting Yang, Han Haolin, Shaoli Wang, Jixing Xia, Wen Xie, Baoyun Xu, Youjun Zhang, Cheng Jiaxu, and Dan Sun
- Subjects
Ecology ,Chive ,Diptera ,Period (gene) ,media_common.quotation_subject ,Temperature ,Gene Expression ,Insect ,Biology ,biology.organism_classification ,Circadian Rhythm ,Cell biology ,Nematocera ,Insect Science ,Eclosion rhythm ,Gene expression ,Animals ,Gene silencing ,Sciaridae ,Circadian rhythm ,PEST analysis ,Ecology, Evolution, Behavior and Systematics ,media_common - Abstract
In most organisms, various physiological and behavioral functions are expressed rhythmically. Previous studies have shown that thermoperiod is an important factor affecting circadian clock-related genes that regulate insect locomotor activity. Bradysia odoriphaga Yang & Zhang is an underground pest that attacks more than 30 crops but is especially damaging to Chinese chives. In this study, we analyzed the adult eclosion time and period (Boper) gene expression in B. odoriphaga as affected by temperature (cycling vs constant temperature), insect stage, and tissue specific. We found that the eclosion time and expression of the Boper gene changed during the temperature cycle but not under a constant temperature. Silencing of Boper expression significantly decreased the adult eclosion rate and significantly increased adult mortality and malformation. The findings indicate that thermoperiod alters Boper expression and regulates the eclosion rhythm.
- Published
- 2021