6 results on '"Djikeng, Appolinaire"'
Search Results
2. Mitochondrial phylogeography and population structure of the cattle tick Rhipicephalus appendiculatus in the African Great Lakes region
- Author
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Amzati, Gaston S., Pelle, Roger, Muhigwa, Jean-Berckmans B., Kanduma, Esther G., Djikeng, Appolinaire, Madder, Maxime, Kirschvink, Nathalie, and Marcotty, Tanguy
- Published
- 2018
- Full Text
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3. First detection of Theileria parva in cattle from Cameroon in the absence of the main tick vector Rhipicephalus appendiculatus.
- Author
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Silatsa, Barberine A., Simo, Gustave, Githaka, Naftaly, Kamga, Rolin, Oumarou, Farikou, Keambou Tiambo, Christian, Machuka, Eunice, Domelevo, Jean‐Baka, Odongo, David, Bishop, Richard, Kuiate, Jules‐Roger, Njiokou, Flobert, Djikeng, Appolinaire, and Pelle, Roger
- Subjects
THEILERIA ,THEILERIA parva ,RHIPICEPHALUS ,CATTLE ,CULICOIDES ,NUCLEIC acid probes ,BLOOD collection ,TICK-borne diseases - Abstract
A major risk factor for the spread of livestock diseases and their vectors is the uncontrolled transboundary movement of live animals for trade and grazing. Such movements constrain effective control of tick‐transmitted pathogens, including Theileria parva. Only limited studies have been undertaken to identify ticks and tick‐borne diseases (TTBDs) affecting cattle in central African countries, including Cameroon. We hereby report the collection of baseline data on the prevalence of T. parva in Cameroon through a countrywide cross‐sectional survey, conducted in 2016, involving collection of blood samples from cattle from 63 sites across the five agro‐ecological zones (AEZs) of the country. ELISA‐based surveillance of infected cattle was performed on 479 randomly selected samples and revealed specific antibodies to T. parva in 22.7% and T. mutans in 41.1% of cattle. Screening of 1,340 representative DNA samples for the presence of T. parva identified 25 (1.86%) positives using a p104 antigen gene‐based nested PCR assay. The positives were distributed across agro‐ecological zones I, II, III and V. None of the p104 positive cattle exhibited clinical symptoms of East Coast fever (ECF). Using reverse line blot (RLB), 58 (4.3%) and 1,139 (85%) of the samples reacted with the T. parva and T. mutans oligonucleotide probes, respectively. This represents the first report of T. parva from Cameroon. Surprisingly, no Rhipicephalus appendiculatus ticks, the main vector of T. parva, were identified in a parallel study involving comprehensive morphological and molecular survey of tick species present in the country. Only two of the 25 p104 positive cattle were PCR‐positive for the CD8+ T‐cell target schizont‐expressed antigen gene Tp1. Cloning and sequencing of Tp1 amplicons revealed sequence identity with the reference T. parva Muguga. This new finding raises serious concerns of a potential spread of ECF into the central African region. [ABSTRACT FROM AUTHOR]
- Published
- 2020
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4. Transcriptomics reveal potential vaccine antigens and a drastic increase of upregulated genes during Theileria parva development from arthropod to bovine infective stages.
- Author
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Tonui, Triza, Corredor-Moreno, Pilar, Kanduma, Esther, Njuguna, Joyce, Njahira, Moses N., Nyanjom, Steven G., Silva, Joana C., Djikeng, Appolinaire, and Pelle, Roger
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THEILERIA parva ,TRANSMISSION of parasitic diseases ,RHIPICEPHALUS appendiculatus ,EAST Coast fever ,ARTHROPODA ,GENETIC regulation ,DISEASE progression - Abstract
Theileria parva is a protozoan parasite transmitted by the brown ear tick Rhipicephalus appendiculatus that causes East Coast fever (ECF) in cattle, resulting in substantial economic losses in the regions of southern, eastern and central Africa. The schizont form of the parasite transforms the bovine host lymphocytes into actively proliferating cancer-like cells. However, how T. parva causes bovine host cells to proliferate and maintain a cancerous phenotype following infection is still poorly understood. On the other hand, current efforts to develop improved vaccines have identified only a few candidate antigens. In the present paper, we report the first comparative transcriptomic analysis throughout the course of T. parva infection. We observed that the development of sporoblast into sporozoite and then the establishment in the host cells as schizont is accompanied by a drastic increase of upregulated genes in the schizont stage of the parasite. In contrast, the ten highest gene expression values occurred in the arthropod vector stages. A comparative analysis showed that 2845 genes were upregulated in both sporozoite and schizont stages compared to the sporoblast. In addition, 647 were upregulated only in the sporozoite whereas 310 were only upregulated in the schizont. We detected low p67 expression in the schizont stage, an unexpected finding considering that p67 has been reported as a sporozoite stage-specific gene. In contrast, we found that transcription of p67 was 20 times higher in the sporoblast than in the sporozoite. Using the expression profiles of recently identified candidate vaccine antigens as a benchmark for selection for novel potential vaccine candidates, we identified three genes with expression similar to p67 and several other genes similar to Tp1—Tp10 schizont vaccine antigens. We propose that the antigenicity or chemotherapeutic potential of this panel of new candidate antigens be further investigated. Structural comparisons of the transcripts generated here with the existing gene models for the respective loci revealed indels. Our findings can be used to improve the structural annotation of the T. parva genome, and the identification of alternatively spliced transcripts. [ABSTRACT FROM AUTHOR]
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- 2018
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5. TpUB05, a Homologue of the Immunodominant Plasmodium falciparum Protein UB05, Is a Marker of Protective Immune Responses in Cattle Experimentally Vaccinated against East Coast Fever.
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Dinga, Jerome Nyhalah, Wamalwa, Mark, Njimoh, Dieudonné Lemuh, Njahira, Moses N., Djikeng, Appolinaire, Skilton, Rob, Titanji, Vincent Pryde Kehdingha, and Pellé, Roger
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PLASMODIUM falciparum ,PROTOZOAN proteins ,BIOMARKERS ,IMMUNE response ,EAST Coast fever ,CATTLE vaccination ,VACCINATION - Abstract
Introduction: East Coast fever, a devastating disease of cattle, can be controlled partially by vaccination with live T. parva sporozoites. The antigens responsible for conferring immunity are not fully characterized. Recently it was shown that the P. falciparum immunodominant protein UB05 is highly conserved in T. parva, the causative agent of East Coast fever. The aim of the present investigation was to determine the role of the homologue TpUB05 in protective immunity to East Coast fever. Methods: The cloning, sequencing and expression of TpUB05 were done according to standard protocols. Bioinformatics analysis of TpUB05 gene was carried out using algorithms found in the public domain. Polyclonal antiserum against recombinant TpUB05 were raised in rabbits and used for further analysis by Western blotting, ELISA, immunolocalization and in vitro infection neutralization assay. The ability of recombinant TpUB05 (r-TpUB05) to stimulate bovine PBMCs ex-vivo to produce IFN-γ or to proliferate was tested using ELISpot and [3H]-thymidine incorporation assays, respectively. Results: All the 20 cattle immunised by the infection and treatment method (ITM) developed significantly higher levels of TpUB05 specific antibodies (p<0.0001) compared to the non-vaccinated ones. Similarly, r-TpUB05 highly stimulated bovine PMBCs from 8/12 (67%) of ITM-immunized cattle tested to produce IFN-γ and proliferate (p< 0.029) as compared to the 04 naїve cattle included as controls. Polyclonal TpUB05 antiserum raised against r-TpUB05 also marginally inhibited infection (p < 0.046) of bovine PBMCs by T. parva sporozoites. In further experiments RT-PCR showed that the TpUB05 gene is expressed by the parasite. This was confirmed by immunolocalization studies which revealed TpUB05 expression by schizonts and piroplasms. Bioinformatics analysis also revealed that this antigen possesses two transmembrane domains, a N-glycosylation site and several O-glycosylation sites. Conclusion: It was concluded that TpUB05 is a potential marker of protective immunity in ECF worth investigating further. [ABSTRACT FROM AUTHOR]
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- 2015
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6. Mitochondrial phylogeography and population structure of the cattle tick <italic>Rhipicephalus appendiculatus</italic> in the African Great Lakes region.
- Author
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Amzati, Gaston S., Pelle, Roger, Muhigwa, Jean-Berckmans B., Kanduma, Esther G., Djikeng, Appolinaire, Madder, Maxime, Kirschvink, Nathalie, and Marcotty, Tanguy
- Subjects
PHYLOGEOGRAPHY ,RHIPICEPHALUS appendiculatus ,EAST Coast fever ,THEILERIA parva ,BIOLOGICAL evolution - Abstract
Background: The ixodid tick
Rhipicephalus appendiculatus is the main vector ofTheileria parva , wich causes the highly fatal cattle disease East Coast fever (ECF) in sub-Saharan Africa.Rhipicephalus appendiculatus populations differ in their ecology, diapause behaviour and vector competence. Thus, their expansion in new areas may change the genetic structure and consequently affect the vector-pathogen system and disease outcomes. In this study we investigated the genetic distribution ofR. appendiculatus across agro-ecological zones (AEZs) in the African Great Lakes region to better understand the epidemiology of ECF and elucidateR. appendiculatus evolutionary history and biogeographical colonization in Africa. Methods: Sequencing was performed on two mitochondrial genes (cox 1 and12S rRNA) of 218 ticks collected from cattle across six AEZs along an altitudinal gradient in the Democratic Republic of Congo, Rwanda, Burundi and Tanzania. Phylogenetic relationships between tick populations were determined and evolutionary population dynamics models were assessed by mismach distribution. Results: Population genetic analysis yielded 22cox 1 and 912S haplotypes in a total of 209 and 126 nucleotide sequences, respectively. Phylogenetic algorithms grouped these haplotypes for both genes into two major clades (lineages A and B). We observed significant genetic variation segregating the two lineages and low structure among populations with high degree of migration. The observed high gene flow indicates population admixture between AEZs. However, reduced number of migrants was observed between lowlands and highlands. Mismatch analysis detected a signature of rapid demographic and range expansion of lineage A. The star-like pattern of isolated and published haplotypes indicates that the two lineages evolve independently and have been subjected to expansion across Africa. Conclusions: Two sympatricR. appendiculatus lineages occur in the Great Lakes region. Lineage A, the most diverse and ubiquitous, has experienced rapid population growth and range expansion in all AEZs probably through cattle movement, whereas lineage B, the less abundant, has probably established a founder population from recent colonization events and its occurrence decreases with altitude. These two lineages are sympatric in central and eastern Africa and allopatric in southern Africa. The observed colonization pattern may strongly affect the transmission system and may explain ECF endemic instability in the tick distribution fringes. [ABSTRACT FROM AUTHOR]- Published
- 2018
- Full Text
- View/download PDF
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