1. An improved PCR method for gender identification of eagles.
- Author
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Chang HW, Chou TC, Gu DL, Cheng CA, Chang CC, Yao CT, Chuang LY, Wen CH, Chou YC, Tan KY, and Cheng CC
- Subjects
- Animals, Base Sequence, DNA Primers, Molecular Sequence Data, Sequence Analysis, DNA, Sex Determination Analysis, Species Specificity, Eagles genetics, Polymerase Chain Reaction methods
- Abstract
Eagles are sexually monomorphic and therefore it is difficult to determine their gender, which is a crucial need for management purposes. In this study, we have developed an improved gender identification method by exploiting length differences between the Chromo-Helicase-DNA binding protein (CHD)-Z and CHD-W genes of Spilornis cheela hoya. By comparing DNA sequences for CHD-W and CHD-Z from 10 species of Falconiformes eagles we designed universal gender identification PCR primers that exploit differences in product size. Standard agarose gels were shown to easily distinguish between the 148-bp CHD-ZW and the 258-bp CHD-W PCR products. When used with 28 samples of S. cheela hoya, our improved universal primers provided a fast and precise gender identification assay.
- Published
- 2008
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