1. Hydroxylated Fluorescent Dyes for Live-Cell Labeling: Synthesis, Spectra and Super-Resolution STED.
- Author
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Butkevich, Alexey N., Belov, Vladimir N., Kolmakov, Kirill, Sokolov, Viktor V., Shojaei, Heydar, Sidenstein, Sven C., Kamin, Dirk, Matthias, Jessica, Vlijm, Rifka, Engelhardt, Johann, and Hell, Stefan W.
- Subjects
FLUORESCENT dyes ,HYDROXYLATION ,ABSORPTION ,RHODAMINES ,CHEMICAL synthesis - Abstract
Hydroxylated rhodamines, carbopyronines, silico- and germanorhodamines with absorption maxima in the range of 530-640 nm were prepared and applied in specific labeling of living cells. The direct and high-yielding entry to germa- and silaxanthones tolerates the presence of protected heteroatoms and may be considered for the syntheses of various sila- and germafluoresceins, as well as -rhodols. Application in stimulated emission depletion (STED) fluorescence microscopy revealed a resolution of 50-75 nm in one- and two-color imaging of vimentin-HaloTag fused protein and native tubulin. The established structure-property relationships allow for prediction of the spectral properties and the positions of spirolactone/zwitterion equilibria for the new analogues of rhodamines, carbo-, silico-, and germanorhodamines using simple additive schemes. [ABSTRACT FROM AUTHOR]
- Published
- 2017
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