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Paper recycling is becoming increasingly important as the production of packaging and various printed products grows. As alternative to wood fiber that is the most common raw material in paper production this research examines the quality of paper which is made with addition of non-wood fibers derived from wheat straw. For this purpose, laboratory paper substrates were made by blending recycled newspaper pulp and wheat straw pulp in different proportions. In this study we analyse rub resistance of laboratory paper substrates with variable content of wheat pulp printed in full tone with black and yellow inks by digital ink jet printing technique. The main objective of this research is to determine whether this kind of print has necessary durability for carrying stable illustrations. One of the main characteristics of print quality is resistance to rubbing which is very important for packaging during transport, storage and the end use. Rub resistance test was executed according to BS 3110 standard. The print durability was assessed through the Euclidean color difference and the individual CIE L*a*b* differences before and after the rub tests. The analysis showed that the increase of wheat pulp content in paper substrates has affected the increase of color difference. Greater rub resistance was observed on substrates with lower share of wheat pulp. Samples printed with yellow ink showed overall greater durability than samples printed with black ink. In further analysis we observed that for the black prints the difference in lightness (ΔL') of the CIE L*a*b* color space was most affected as the prints became lighter with the number of rubbing cycles. In samples printed with yellow ink most affected was the color difference on yellow-blue axis (Δb') meaning that the yellowness of the prints was degrading with the number of rubbing cycles. [ABSTRACT FROM AUTHOR]