1. Synergistic Amelioration of Letrozole-induced Polycystic Ovary Syndrome in Rats: A Therapeutic Approach with Apple Cider Vinegar and Metformin Combination.
- Author
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Danduga RCSR, Kurapati AS, Shaik RA, Kola PK, Konidala SK, and Varada HB
- Subjects
- Animals, Female, Rats, Ovary drug effects, Ovary pathology, Ovary metabolism, Malus chemistry, Blood Glucose drug effects, Blood Glucose metabolism, Aromatase Inhibitors pharmacology, Aromatase Inhibitors administration & dosage, Estrous Cycle drug effects, Polycystic Ovary Syndrome chemically induced, Polycystic Ovary Syndrome drug therapy, Polycystic Ovary Syndrome pathology, Letrozole administration & dosage, Metformin pharmacology, Metformin administration & dosage, Rats, Wistar, Acetic Acid, Oxidative Stress drug effects, Hypoglycemic Agents pharmacology, Hypoglycemic Agents administration & dosage, Drug Synergism, Drug Therapy, Combination
- Abstract
The present study was conducted to evaluate the combination effect of apple cider vinegar (ACV) and metformin against letrozole-induced polycystic ovary syndrome (PCOS). Female Wistar rats were administered letrozole (1 mg/kg/day, p.o) for 21 days, except for the control group of animals. On the 22nd day, PCOS-induced animals were segregated into 4 groups and administered with CMC, ACV, metformin, and a combination of ACV and metformin, respectively. The treatments were continued for 15 days, and on the 36th day, all the animals were sacrificed for biochemical (blood glucose, lipid profile), hormonal (sex hormones and adiponectin), and pro-inflammatory mediator estimations in blood samples. The ovarian tissue samples were used for oxidative stress parameters and histological alterations. The PCOS control animals showed a significant alteration in the estrous cycle. The administration of letrozole resulted in the alteration of hormonal balance and elevation of body weights, glycemic state, lipid profile, pro-inflammatory mediators in serum, and oxidative stress in ovarian samples. Individual treatment groups and combination treatment groups reversed the letrozole-induced alterations in PCOS animals, and more promising results were observed with combination therapy than with individual treatment groups. Further, the therapeutic potential of the combination treatment group was also confirmed by the histological observations in the ovarian samples. The study showed that the combination of ACV and metformin significantly alleviated letrozole-induced PCOS complications in rats. This might have been achieved by mitigating the hormonal imbalance, pro-inflammatory, hyperglycemic, and hyperlipidemic states in serum, and oxidative stress in the ovary samples., (© 2024. The Author(s), under exclusive licence to Society for Reproductive Investigation.)
- Published
- 2024
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